Análise da expressão gênica e atividade enzimática antioxidante em Passiflora edulis SIMS sob diferentes concentrações de alumínio

• Ano de defesa: 2012
• Autor(a) principal: Pretti, Irany Rodrigues
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Biologia Vegetal; UFES; Programa de Pós-Graduação em Biologia Vegetal
• Palavras-chave em Português: Passion fruit; Aluminium; Proteomics; Ascorbate peroxidase; Superoxide dismutase; Proteômica; Peroxidase do ascorbato; Catalase; mRNA; Superóxido desmutase; Peroxidase; Maracujá; Alumínio; Agronomia; 57
• Link de acesso: http://repositorio.ufes.br/handle/10/9998

The passion fruit is cultivated in almost all the brazilian territory. The Espírito Santo State currently ranks fourth in production in the country. The production of Espírito Santo, Passiflora edulis, is expanding, given that in recent years there has been an increase in planted area of 500 ha of passion, in 1990 to 2,429 ha in March 2008, reaching 2010 in December, 2800 ha planted. However, most of the production in Espírito Santo is still concentrated in the northern region of the state, especially the municipalities of the Jaguaré and Sooretama. While the numbers show progress in the production of fruit, the Espírito Santo is characterized by acidic soils and high aluminum content. Among the responses triggered by exposure to aluminum are: inhibition of root growth, inhibition of cell division, nutrient deficiency, and the activation of signaling pathways and changes in level of protein and RNA transcripts. Thus, it becomes important to study the components of the antioxidant defense system in plants undergoing exposure to aluminum. The ROS can pose serious threat to the cell, one of the responses to environmental stresses such as toxic aluminum. However, few studies have reported the effects of Al on the plant canopy. Therefore, the objective of this study was to evaluate the response of the antioxidant system P.edulis under such conditions. The study of the oxidative stress induced by aluminum showed that the CAT and SOD gene expression of was increased in plants in the nutrient solution and only cv. FB100 this increase was accompanied by increment enzymatic activity of SOD, indicating the higher efficiency of the removal of ROS. Plants grown in the field showed that the expression of antioxidant enzymes in the crop with high aluminum content was not activated at the time of collection, despite the high activity of SOD and APX. This response was possibly determined by the extended time to which the plants were exposed to stress.