Determinação do perfil cromatográfico e avaliação da atividade do extrato etanólico de Achillea millefolium sobre Colletotrichum gloeosporioides

• Ano de defesa: 2013
• Autor(a) principal: Duque, Fernanda Farias
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Agricultura Tropical; UFES; Programa de Pós-Graduação em Agricultura Tropical
• Palavras-chave em Português: Yarrow; Anthracnose; Bioassays; Mil-folhas; Bioensaios; Fungicidas; Antracnose; Colletotrichum gloeosporioides; Análise cromatográfica; Agronomia; 63
• Link de acesso: http://repositorio.ufes.br/handle/10/5598

The Achillea millefolium, known as mil-folhas or aquilea, is a plant of the Asteraceae family and possesses activity as antibacterial, antifungal, antitumor, healing, antioxidant and antiedematosa. The aim of this study was to determine the elution profile and evaluate the in vitro antifungal activity of the ethanol extract of A. millefolium on Colletotrichum gloeosporioides causing anthracnose on papaya fruits. Parts of the plant were collected in two different months and separated into flowers, leaves and branches. Bioassays were performed with dry ethanolic extracts obtained by maceration technique and concentrated in a rotary evaporator under reduced pressure. These extracts were subjected to preparative thin layer chromatography (TLC) was obtained five fractions. These fractions were diluted separately in 150μL of DMSO and incorporated in BDA. After solidification of the medium, a disc of 0.6 cm diameter with the fungus was placed in the center of a petri dish and incubated at 28 ± 2°C. After growth evaluated the radial growth of the mycelium. The experimental design was factorial analysis and the means were compared by Tukey test at 5% probability. The antifungal activity was observed against C. gloeosporioides whereas fractions F4 and F5 of leaf and flower collected in october had the highest percentage of mycelial growth inhibition. The chromatographic profile of the fractions of the extract was performed by analysis using HPLC-DAD and GC-MS. Through analysis using HPLC and GC leaf collected in october was possible to detect the presence of coumaric acid, ferulic acid and rutin in fractions F4 and F5.