Análise da distribuição espacial de designer drugs em selos por MALDI FT-ICR Imaging MS

• Ano de defesa: 2020
• Autor(a) principal: Almeida, Camila Medeiros de
• Orientador(a): Romão, Wanderson
• Banca de defesa: Lopes, Norberto Peporine , Borges, Warley de Souza
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; Mestrado em Química
• Programa de Pós-Graduação: Programa de Pós-Graduação em Química
• Departamento: Centro de Ciências Exatas
• País: BR
• Palavras-chave em Português: Designer drugs; 25I-NBOMe; 25I-NBOH; MALDI MSI
• Área do conhecimento CNPq: Química
• Link de acesso: http://repositorio.ufes.br/handle/10/15001

The phenethylamines derivatives, the NBOMes, N-bomb or Smiles, are potent hallucinogens, which are often sold as blotter paper. Changes in their molecular structures are constantly carried out, such as the exchange of halogen in the carbon 4 (C4) or the substitution of the methyl to a hydroxyl, observed in the modification of NBOMe to NBOH. This happens so that the new substances are not classified as illicit and preserved the psychoactive effects of molecules. In order to follow the dynamics of designer drugs market, the use of different analytical methods for the identification of these substances has been a solution. In this study, the matrix assisted laser desorption/ionization mass spectrometry (MALDI MS) and MALDI mass spectrometry imaging (MALDI MSI) were coupled to a Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) and used to analyze seven blotter papers of NBOMes containing 25I-NBOH (2-({[2-(4-iodo-2,5- dimethoxyphenyl) ethyl]amino}methyl) phenol, m/z 414,05) and 25I-NBOMe ((2-(4-iodo-2,5-dimethoxyphenyl)-N-[(2 methoxyphenyl) methyl]ethanamine), m/z 428,07). An optimization study was performed to evaluate the matrix application (pipette versus sprayer assisted by an ESI probe); the matrices (α-cyano-4-hydroxycinnamic acid (CHCA), 2,5 dihydroxybenzoic acid (DHB), 7,7,8,8-tetracyanoquinodimethane (TCNQ) and sinapinic acid (SA)) effect in the process of desorption and ionization and their concentration (from 5 to 25 mg.mL−1). After, a study of the spatial distribution of the NBOMes derivatives detected in the front and back of the blotter paper surface was performed. MALDI MS and MALDI MSI results showed that the matrix sprayer was the best method for matrix application, which allowed a homegeneous detection of the compound when compared to the pipette application. The CHCA matrix, in a concentration ≥ 15 mg.mL−1, displayed a higher ionization efficiency of the 25I-NBOMe compound, commonly detected in blotter paper, where an intense distribution was observed in relation to others matrices. The distribution of the active ingredient studied in the front and back between the six blotter papers analyzed showed that the three of them had a higher concentration of the active ingredient in only one of the sides (two in the back and one in the front), while the others presented a similar distribution in both sides.