Eficácia de isolados bacterianos na mortalidade de Meloidogyne exigua

• Ano de defesa: 2023
• Autor(a) principal: Silva, Milena Barbosa Parreira da
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Agronomia; Centro de Ciências Agrárias e Engenharias; UFES; Programa de Pós-Graduação em Agronomia
• Palavras-chave em Português: Nematoide das galhas; Isolados bacterianos; Controle biológico; subject.br-rjbn; Agronomia
• Link de acesso: http://repositorio.ufes.br/handle/10/16942

One of the most used methods for managing M. exigua is through the application of chemical nematicides, however, these products are harmful to animals, humans and the environment, which is why alternative ways of managing this pathogen have been sought, such as the use ofantagonistic bacteria. Two experiments (experiments 1 and 2) were carried out and repeated once. In the first, the effect of bacterial isolates obtained from commercial biofertilizers on themortality of M. exigua 'in vitro' was studied. The experimental design was completely randomized (CRD) with 22 treatments (20 bacterial isolates + one positive control + one negative control) and four replications, totaling 88 experimental units. Seven out of twenty bacterial isolates (B74, B75, B84, B85, B86, B88, B121), demonstrated effect on the mortalityof M. exigua J2s after 24 hours of incubation. Bacteria B74, B75, B84, B86 and B88 were identified and belong to the genus Bacillus sp., whereas isolates B85 and B121 were identifiedas Micrococcus sp. and Brevibacterium epidermidis, respectively. Isolates B88, B74, B121, B86 showed mortality percentages greater than 75%, whereas B75, B84 and B85 reached percentages greater than 57%. In the second experiment, the selection and compatibility test was carried out between the most promising bacterial isolates antagonistic to M. exigua in experiment 1. The experimental design was CRD with 9 treatments (7 bacterial isolates + one positive control + one negative control) and four repetitions. There was no antagonistic effect among the studied bacterial isolates. The results of experiments 1 and 2 show the potential, mainly of isolates B121, B74, B86, B88, as possible biological control agents for M. exigua and indicate that these bacteria can be used in combination for the management of phytonematodes, as they do not have an antagonistic effect on each other.