Avaliação da freqüência de displasia epitelial em hiperplasias fibrosas inflamatórias e leucoplasias: aspectos histopatológicos e imuno-histoquímicos
Ano de defesa: | 2009 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Programa de Pós-graduação em Patologia
Patologia |
Programa de Pós-Graduação: |
Não Informado pela instituição
|
Departamento: |
Não Informado pela instituição
|
País: |
Não Informado pela instituição
|
Palavras-chave em Português: | |
Link de acesso: | https://app.uff.br/riuff/handle/1/18724 |
Resumo: | Cancer development in the oral mucosa may be preceded by epithelial abnormalities ranging from hyperplasia to intraepithelial neoplasia termed histologically epithelial dysplasia. Epithelial dysplasia is characterized by architectural disturbance accompanied by cytological atypia and is generally regarded as one of the most important predictors of malignant transformation in the potentially malignant oral lesions. Dysplasia is a spectrum and no criteria exist to precisely divide this spectrum into mild, moderate and severe categories. It is accepted that the more severe the dysplasia the greater the likelihood is of progression to malignancy. The presence of epithelial dysplasia and its classification must be reported. However, evaluation of dysplastic features is subjective and considerable inter-and intra-observer variations in the scoring of epithelial dysplasia have been reported. In the last decades, several studies have been evaluating the expression of molecular markers that may identify high-risk lesions. The aim of this study was to investigate the frequency and degree of epithelial dysplasia in inflammatory fibrous hyperplasia and leukoplakias, as well as to compare the immunostaining of the antibodies against Ki-67 protein and p53 protein in cases with and without epithelial dysplasia. A retrospective study was performed with 138 specimens diagnosed as inflammatory fibrous hyperplasia and 19 as compatible with the clinical diagnosis of leukoplakia . Blocks of Tissue Microarray (TMA) modified for bigger specimens were constructed for the cases of inflammatory fibrous hyperplasia. Immunohistochemical staining method was used to evaluate the expression of Ki-67 and p53 proteins and the quantification and localization of the immunostaining were related with the presence of epithelial dysplasia. A p value ≤ .05 was considered to indicate statistical significance. Presence of epithelial dysplasia was observed in 13.8% and 52.6% of inflammatory fibrous hyperplasias and leukoplakias, respectively. Moderate epithelial dysplasia was noted in three cases of inflammatory fibrous hyperplasia and all of the remains were graded as mild dysplasia. Ki-67 and p53 proteins were expressed in 97.1% and 97.8% of the inflammatory fibrous hyperplasias, respectively, and in all of leukoplakias. It was noted that the labeling index and the ratio of cases with suprabasal Ki-67 immunoexpression in the hyperplasias with dysplasia were significantly bigger than cases without dysplasia. The p53 labeling index was bigger in leukoplakias with dysplasia than in leukoplakias without dysplasia. However, suprabasal p53 immunostaining was not able to distinguish cases with dysplasia from these without dysplasia. In conclusion, the frequency of epithelial dysplasia in inflammatory fibrous hyperplasia is greater than specified in literature and our results suggest that increase and localization of Ki-67 immunostaining can assist in the diagnosis of the presence/absence of epithelial dysplasia in this group of lesions. The increase of p53 expression in potentially malignant lesions can be associated with early events of oral carcinogenesis |
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Avaliação da freqüência de displasia epitelial em hiperplasias fibrosas inflamatórias e leucoplasias: aspectos histopatológicos e imuno-histoquímicosHiperplasia Fibrosa InflamatóriaDisplasia epitelialLeucoplasiaHistopatologiaImuno-histoquímicaProteína p53Antígeno Ki-67inflammatory Fibrous HyperplasiaLeukoplakiaEpithelial dysplasiaHistopathologyImmunohistochemistryP53 proteinKi-67 antigenCNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICACancer development in the oral mucosa may be preceded by epithelial abnormalities ranging from hyperplasia to intraepithelial neoplasia termed histologically epithelial dysplasia. Epithelial dysplasia is characterized by architectural disturbance accompanied by cytological atypia and is generally regarded as one of the most important predictors of malignant transformation in the potentially malignant oral lesions. Dysplasia is a spectrum and no criteria exist to precisely divide this spectrum into mild, moderate and severe categories. It is accepted that the more severe the dysplasia the greater the likelihood is of progression to malignancy. The presence of epithelial dysplasia and its classification must be reported. However, evaluation of dysplastic features is subjective and considerable inter-and intra-observer variations in the scoring of epithelial dysplasia have been reported. In the last decades, several studies have been evaluating the expression of molecular markers that may identify high-risk lesions. The aim of this study was to investigate the frequency and degree of epithelial dysplasia in inflammatory fibrous hyperplasia and leukoplakias, as well as to compare the immunostaining of the antibodies against Ki-67 protein and p53 protein in cases with and without epithelial dysplasia. A retrospective study was performed with 138 specimens diagnosed as inflammatory fibrous hyperplasia and 19 as compatible with the clinical diagnosis of leukoplakia . Blocks of Tissue Microarray (TMA) modified for bigger specimens were constructed for the cases of inflammatory fibrous hyperplasia. Immunohistochemical staining method was used to evaluate the expression of Ki-67 and p53 proteins and the quantification and localization of the immunostaining were related with the presence of epithelial dysplasia. A p value ≤ .05 was considered to indicate statistical significance. Presence of epithelial dysplasia was observed in 13.8% and 52.6% of inflammatory fibrous hyperplasias and leukoplakias, respectively. Moderate epithelial dysplasia was noted in three cases of inflammatory fibrous hyperplasia and all of the remains were graded as mild dysplasia. Ki-67 and p53 proteins were expressed in 97.1% and 97.8% of the inflammatory fibrous hyperplasias, respectively, and in all of leukoplakias. It was noted that the labeling index and the ratio of cases with suprabasal Ki-67 immunoexpression in the hyperplasias with dysplasia were significantly bigger than cases without dysplasia. The p53 labeling index was bigger in leukoplakias with dysplasia than in leukoplakias without dysplasia. However, suprabasal p53 immunostaining was not able to distinguish cases with dysplasia from these without dysplasia. In conclusion, the frequency of epithelial dysplasia in inflammatory fibrous hyperplasia is greater than specified in literature and our results suggest that increase and localization of Ki-67 immunostaining can assist in the diagnosis of the presence/absence of epithelial dysplasia in this group of lesions. The increase of p53 expression in potentially malignant lesions can be associated with early events of oral carcinogenesisO desenvolvimento de câncer na mucosa oral é, freqüentemente, precedido por alterações epiteliais que variam desde hiperplasia até neoplasia intra-epitelial, denominadas pelo termo histopatológico displasia epitelial. A displasia epitelial é caracterizada por um distúrbio arquitetural acompanhado de atipias citológicas, sendo considerada um importante indicador do risco de transformação maligna nas lesões potencialmente malignas orais. Displasia é um espectro e não existe um critério reproduzível que a separe, precisamente, em categorias leve, moderada e severa. Acredita-se que o potencial para o desenvolvimento de um carcinoma invasivo aumenta com a severidade da displasia epitelial. Dessa forma, a presença de displasia e sua graduação devem sempre ser relatadas. Entretanto, a avaliação das características displásicas é extremamente subjetiva e têm sido descritas consideráveis variações entre e intra-observadores em sua classificação. Nas últimas décadas, muitos trabalhos têm avaliado a expressão de marcadores moleculares que possam identificar lesões de alto risco para transformação maligna. Com o objetivo de investigar a freqüência e o grau de displasia epitelial em hiperplasias fibrosas inflamatórias e em leucoplasias, bem como comparar a imunomarcação dos anticorpos anti-Ki-67 e anti-p53 nos casos com e sem displasia epitelial, realizou-se um estudo retrospectivo com 138 espécimes de biópsia diagnosticados como hiperplasia fibrosa inflamatória e 19 como compatível com o diagnóstico clínico de leucoplasia . Para os casos de hiperplasia fibrosa inflamatória foram construídos blocos de Tissue Microarray (TMA) modificados para fragmentos maiores. A expressão das proteínas Ki-67 e p53 foi avaliada por imuno-histoquímica e a quantificação e localização das imunomarcações foram relacionadas com a presença de displasia epitelial. As decisões estatísticas foram tomadas ao nível de significância de 0,05 (5%). Na análise histopatológica, observou-se a presença de displasia epitelial em 13,8% e 52,6% dos casos de hiperplasia fibrosa inflamatória e leucoplasia, respectivamente. Apenas três casos de hiperplasia fibrosa inflamatória foram classificados como displasia moderada e todos os restantes foram classificados como displasia leve. As proteínas Ki-67 e p53 foram expressas em 97,1% e 97,8% dos casos de hiperplasia, respectivamente, e em todos os casos de leucoplasia. Observou-se que o índice de positividade e a proporção de casos com imunomarcação suprabasal pelo anticorpo anti-Ki-67 nas hiperplasias com displasia foram significantemente maiores do que os casos sem displasia. O índice de positividade do anticorpo anti-p53 foi maior nas leucoplasias com displasia do que nas leucoplasias sem displasia. Entretanto, a imunomarcação suprabasal pelo anticorpo anti-p53 não foi capaz de distinguir casos com displasia dos casos sem displasia. Pôde-se concluir que a freqüência de displasia epitelial em hiperplasias fibrosas inflamatórias é maior que a relatada na literatura e nossos resultados sugerem que o aumento e a localização da imunomarcação pelo anticorpo anti-Ki-67 podem auxiliar no diagnóstico da presença/ausência de displasia nesse grupo de lesões. O aumento da expressão da proteína p53 nas lesões potencialmente malignas pode estar associado aos estágios iniciais da carcinogênese oralPrograma de Pós-graduação em PatologiaPatologiaDias, Eliane Pedrahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786983U3&dataRevisao=nullPires, Fábio Ramôahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4768254A9&dataRevisao=nullCantisano, Marilia Hefferhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4778292E3Pires, Andréa Rodrigues Cordovilhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4237114H3Fraga, Samira Regina Guimarães2021-03-10T20:45:24Z2010-02-082021-03-10T20:45:24Z2009-02-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://app.uff.br/riuff/handle/1/18724porCC-BY-SAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF)instname:Universidade Federal Fluminense (UFF)instacron:UFF2023-03-01T18:22:06Zoai:app.uff.br:1/18724Repositório InstitucionalPUBhttps://app.uff.br/oai/requestriuff@id.uff.bropendoar:21202023-03-01T18:22:06Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF)false |
dc.title.none.fl_str_mv |
Avaliação da freqüência de displasia epitelial em hiperplasias fibrosas inflamatórias e leucoplasias: aspectos histopatológicos e imuno-histoquímicos |
title |
Avaliação da freqüência de displasia epitelial em hiperplasias fibrosas inflamatórias e leucoplasias: aspectos histopatológicos e imuno-histoquímicos |
spellingShingle |
Avaliação da freqüência de displasia epitelial em hiperplasias fibrosas inflamatórias e leucoplasias: aspectos histopatológicos e imuno-histoquímicos Fraga, Samira Regina Guimarães Hiperplasia Fibrosa Inflamatória Displasia epitelial Leucoplasia Histopatologia Imuno-histoquímica Proteína p53 Antígeno Ki-67 inflammatory Fibrous Hyperplasia Leukoplakia Epithelial dysplasia Histopathology Immunohistochemistry P53 protein Ki-67 antigen CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA |
title_short |
Avaliação da freqüência de displasia epitelial em hiperplasias fibrosas inflamatórias e leucoplasias: aspectos histopatológicos e imuno-histoquímicos |
title_full |
Avaliação da freqüência de displasia epitelial em hiperplasias fibrosas inflamatórias e leucoplasias: aspectos histopatológicos e imuno-histoquímicos |
title_fullStr |
Avaliação da freqüência de displasia epitelial em hiperplasias fibrosas inflamatórias e leucoplasias: aspectos histopatológicos e imuno-histoquímicos |
title_full_unstemmed |
Avaliação da freqüência de displasia epitelial em hiperplasias fibrosas inflamatórias e leucoplasias: aspectos histopatológicos e imuno-histoquímicos |
title_sort |
Avaliação da freqüência de displasia epitelial em hiperplasias fibrosas inflamatórias e leucoplasias: aspectos histopatológicos e imuno-histoquímicos |
author |
Fraga, Samira Regina Guimarães |
author_facet |
Fraga, Samira Regina Guimarães |
author_role |
author |
dc.contributor.none.fl_str_mv |
Dias, Eliane Pedra http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786983U3&dataRevisao=null Pires, Fábio Ramôa http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4768254A9&dataRevisao=null Cantisano, Marilia Heffer http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4778292E3 Pires, Andréa Rodrigues Cordovil http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4237114H3 |
dc.contributor.author.fl_str_mv |
Fraga, Samira Regina Guimarães |
dc.subject.por.fl_str_mv |
Hiperplasia Fibrosa Inflamatória Displasia epitelial Leucoplasia Histopatologia Imuno-histoquímica Proteína p53 Antígeno Ki-67 inflammatory Fibrous Hyperplasia Leukoplakia Epithelial dysplasia Histopathology Immunohistochemistry P53 protein Ki-67 antigen CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA |
topic |
Hiperplasia Fibrosa Inflamatória Displasia epitelial Leucoplasia Histopatologia Imuno-histoquímica Proteína p53 Antígeno Ki-67 inflammatory Fibrous Hyperplasia Leukoplakia Epithelial dysplasia Histopathology Immunohistochemistry P53 protein Ki-67 antigen CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA |
description |
Cancer development in the oral mucosa may be preceded by epithelial abnormalities ranging from hyperplasia to intraepithelial neoplasia termed histologically epithelial dysplasia. Epithelial dysplasia is characterized by architectural disturbance accompanied by cytological atypia and is generally regarded as one of the most important predictors of malignant transformation in the potentially malignant oral lesions. Dysplasia is a spectrum and no criteria exist to precisely divide this spectrum into mild, moderate and severe categories. It is accepted that the more severe the dysplasia the greater the likelihood is of progression to malignancy. The presence of epithelial dysplasia and its classification must be reported. However, evaluation of dysplastic features is subjective and considerable inter-and intra-observer variations in the scoring of epithelial dysplasia have been reported. In the last decades, several studies have been evaluating the expression of molecular markers that may identify high-risk lesions. The aim of this study was to investigate the frequency and degree of epithelial dysplasia in inflammatory fibrous hyperplasia and leukoplakias, as well as to compare the immunostaining of the antibodies against Ki-67 protein and p53 protein in cases with and without epithelial dysplasia. A retrospective study was performed with 138 specimens diagnosed as inflammatory fibrous hyperplasia and 19 as compatible with the clinical diagnosis of leukoplakia . Blocks of Tissue Microarray (TMA) modified for bigger specimens were constructed for the cases of inflammatory fibrous hyperplasia. Immunohistochemical staining method was used to evaluate the expression of Ki-67 and p53 proteins and the quantification and localization of the immunostaining were related with the presence of epithelial dysplasia. A p value ≤ .05 was considered to indicate statistical significance. Presence of epithelial dysplasia was observed in 13.8% and 52.6% of inflammatory fibrous hyperplasias and leukoplakias, respectively. Moderate epithelial dysplasia was noted in three cases of inflammatory fibrous hyperplasia and all of the remains were graded as mild dysplasia. Ki-67 and p53 proteins were expressed in 97.1% and 97.8% of the inflammatory fibrous hyperplasias, respectively, and in all of leukoplakias. It was noted that the labeling index and the ratio of cases with suprabasal Ki-67 immunoexpression in the hyperplasias with dysplasia were significantly bigger than cases without dysplasia. The p53 labeling index was bigger in leukoplakias with dysplasia than in leukoplakias without dysplasia. However, suprabasal p53 immunostaining was not able to distinguish cases with dysplasia from these without dysplasia. In conclusion, the frequency of epithelial dysplasia in inflammatory fibrous hyperplasia is greater than specified in literature and our results suggest that increase and localization of Ki-67 immunostaining can assist in the diagnosis of the presence/absence of epithelial dysplasia in this group of lesions. The increase of p53 expression in potentially malignant lesions can be associated with early events of oral carcinogenesis |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-02-19 2010-02-08 2021-03-10T20:45:24Z 2021-03-10T20:45:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://app.uff.br/riuff/handle/1/18724 |
url |
https://app.uff.br/riuff/handle/1/18724 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
CC-BY-SA info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
CC-BY-SA |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Programa de Pós-graduação em Patologia Patologia |
publisher.none.fl_str_mv |
Programa de Pós-graduação em Patologia Patologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF) instname:Universidade Federal Fluminense (UFF) instacron:UFF |
instname_str |
Universidade Federal Fluminense (UFF) |
instacron_str |
UFF |
institution |
UFF |
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Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
collection |
Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF) |
repository.mail.fl_str_mv |
riuff@id.uff.br |
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1797038182097747968 |