Avaliação da regeneração hepática após isquemia/reperfusão hepática e hepatectomia sob influência do recondicionamento isquêmico

• Ano de defesa: 2011
• Autor(a) principal: Salomão, Laura Sampaio
• Orientador(a): Martinho, Jose Manoel da Silva Gomes
• Banca de defesa: Boaventura, Gilson Teles , Moreira, Lucio Filgueiras Pacheco , Palombo, Anielo
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Programa de Pós-Graduação: Programa de Pós-graduação em Ciências Médicas
• Departamento: Ciências Médicas
• Palavras-chave em Português: Regeneração; Fígado; Precondicionamento isquêmico; MEDICINA; CIÊNCIAS MÉDICAS; Hepatectomia; Regeneração hepática
• Palavras-chave em Inglês: Regeneration; Liver; Ischemic preconditioning
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE
• Link de acesso: https://app.uff.br/riuff/handle/1/19395

This study aimed to evaluate liver regeneration modulation by ischemic preconditioning after ischemia and reperfusion and partial hepatectomy. 24 female Wistar rats of 12 weeks of age were assigned randomly into four groups: (SHAM) laparotomy, surgical simulation and observation under anesthesia for 60 m inutes (HEP) partial hepatectomy after 55 minutes of the opening of the cavity, (GIR) Ischemia liver for 30 minutes and after partial hepatectomy, (PRE) 10/10 minute ischemic preconditioning (ischemia / reperfusion) followed by 30 minutes of ischemia and subsequent partial hepatectomy . In all animals, laparotomy was performed and section of the falciform and left coronary ligaments and the identification and isolation of the hepatic pedicle. After 48 hours, the animals were relaparotomized to collect blood for determination of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and removal of the remaining lobes of the liver for evaluation of regeneration through the initial and final weight of the liver and to study the proliferation of hepatocytes by immunohistochemistry (PCNA). For the analysis of results and comparison between groups, was applied the Mann-Whitney test. It was adopted significance level of 0.05 or 5% (P <0.05). In all groups there were high rates of regeneration, and the PRE group had the highest rate. There were significant differences for ALT and AST between the groups HEP-SHAM, GIR-PRE, GIR-SHAM E PRE-SHAM (P <0.05). With respect to the enzyme ALT and AST compared with the control group, there was an increase, which characterizes liver damage due to surgery. There were also significant differences in the PCNA labeling of the SHAM group when compared to other groups