FGFb E O CRESCIMENTO DE PROCESSOS DAS CÉLULAS DA RETINA
| Ano de defesa: | 2009 |
|---|---|
| Autor(a) principal: | |
| Orientador(a): | |
| Banca de defesa: | |
| Tipo de documento: | Dissertação |
| Tipo de acesso: | Acesso aberto |
| dARK ID: | ark:/87559/0013000002wvq |
| Idioma: | por |
| Instituição de defesa: |
Programa de Pós-graduação em Neuroimunologia
Neuroimunologia |
| Programa de Pós-Graduação: |
Não Informado pela instituição
|
| Departamento: |
Não Informado pela instituição
|
| País: |
Não Informado pela instituição
|
| Palavras-chave em Português: | |
| Link de acesso: | https://app.uff.br/riuff/handle/1/18463 |
Resumo: | Basic fibroblast growth factor plays an important role during the development of the nervous system mediating different events such as proliferation, migration, survival and neurite outgrowth. The aim of this work was to analyze the effect of basic fibroblast growth factor on the outgrowth of processes from neonatal retinal cells kept in culture. We also investigated the intracellular pathways involved in this effect. Our results clearly show that 25ng/mL bFGF increases the amount of cells with process greater than cell soma but, the total number of the cells was not affected. The inhibitors of MEK (50μM PD98059), PI3-kinase (25μM LY294002), PKC (1,25μM queleritrine) and Src (1,0μM PP1) blocked completely the effect of bFGF whereas the inhibitors of epidermal growth factor receptor (2,5μM tyrfostin), L type Ca2+ channel (5,0μM nifedipine) and intracellular Ca 2+ chelator (20μM BAPTA-AM) were not able to blocky the effect of bFGF. An interesting effect was observed when cells were treated with a p38 inhibitor (20μM SB 202190). This drug induced an increase in process outgrowth and when incubated with bFGF we observed a sinergistic effect. Our results indicate that MEK, PI3-kinase, PKC and Src play an important role in the effect of FGFb on process outgrowth from retinal cells. |
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FGFb E O CRESCIMENTO DE PROCESSOS DAS CÉLULAS DA RETINAFGFb AND GROWTH OF CASES OF CELLS OF THE RETINASistema nervosoDesenvolvimento da retinaFGFbretina animalFibroblastoNervous system, development of the retinaFGFbanimal retinaFibroblastCNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIABasic fibroblast growth factor plays an important role during the development of the nervous system mediating different events such as proliferation, migration, survival and neurite outgrowth. The aim of this work was to analyze the effect of basic fibroblast growth factor on the outgrowth of processes from neonatal retinal cells kept in culture. We also investigated the intracellular pathways involved in this effect. Our results clearly show that 25ng/mL bFGF increases the amount of cells with process greater than cell soma but, the total number of the cells was not affected. The inhibitors of MEK (50μM PD98059), PI3-kinase (25μM LY294002), PKC (1,25μM queleritrine) and Src (1,0μM PP1) blocked completely the effect of bFGF whereas the inhibitors of epidermal growth factor receptor (2,5μM tyrfostin), L type Ca2+ channel (5,0μM nifedipine) and intracellular Ca 2+ chelator (20μM BAPTA-AM) were not able to blocky the effect of bFGF. An interesting effect was observed when cells were treated with a p38 inhibitor (20μM SB 202190). This drug induced an increase in process outgrowth and when incubated with bFGF we observed a sinergistic effect. Our results indicate that MEK, PI3-kinase, PKC and Src play an important role in the effect of FGFb on process outgrowth from retinal cells.O fator de crescimento de fibroblasto básico desempenha um importante papel durante o desenvolvimento do sistema nervoso mediando diferentes eventos, tais como: proliferação, migração, sobrevida e crescimento de neuritos. O objetivo deste trabalho foi analisar o efeito do fator de crescimento de fibroblasto básico no crescimento de processos das células da retina de ratos neonatos mantidas em cultura, bem como identificar as vias de sinalizações envolvidas nesse efeito. Nossos resultados demonstram claramente que o FGFb 25ng/mL aumenta a quantidade de células com processos maiores que o corpo celular sem que haja aumento do número total de células. Os inibidores da MEK (PD 98059 - 50μM), da PI3-cinase (LY294003 - 25μM), da PKC (Cloreto de queleritrina 1,25μM) e da Src (PP1 1,0μM) aboliram completamente o efeito do FGFb. Entretanto, os bloqueadores dos receptores do fator de crescimento da epiderme (tirfostina 2,5μM), dos canais de Ca2+ do tipo L (nifedipina - 5μM) e o quelante de cálcio intracelular (BAPTA-AM - 20μM) não foram capazes de bloquear o crescimento de processos induzido pelo FGFb. Dado interessante é que o inibidor da via da p38 (SB 202190 - 20μM) foi capaz de aumentar também o número de células com processos e quando foi incubado com FGFb houve uma somação dos efeitos. Baseados em nossos resultados podemos concluir que há participação das vias da MEK, da PI3- cinase, da PKC e da Src no efeito do FGFb sobre o crescimento de processos de células da retina mantidas em cultura.Programa de Pós-graduação em NeuroimunologiaNeuroimunologiaAraújo, Elizabeth Giestal deCPF:55544433122http://lattes.cnpq.br/8230334072312477Souza, Andreza Rozendo de2021-03-10T20:44:39Z2009-07-272021-03-10T20:44:39Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://app.uff.br/riuff/handle/1/18463ark:/87559/0013000002wvqporCC-BY-SAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF)instname:Universidade Federal Fluminense (UFF)instacron:UFF2021-03-10T20:44:39Zoai:app.uff.br:1/18463Repositório InstitucionalPUBhttps://app.uff.br/oai/requestriuff@id.uff.bropendoar:21202021-03-10T20:44:39Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF)false |
| dc.title.none.fl_str_mv |
FGFb E O CRESCIMENTO DE PROCESSOS DAS CÉLULAS DA RETINA FGFb AND GROWTH OF CASES OF CELLS OF THE RETINA |
| title |
FGFb E O CRESCIMENTO DE PROCESSOS DAS CÉLULAS DA RETINA |
| spellingShingle |
FGFb E O CRESCIMENTO DE PROCESSOS DAS CÉLULAS DA RETINA Souza, Andreza Rozendo de Sistema nervoso Desenvolvimento da retina FGFb retina animal Fibroblasto Nervous system, development of the retina FGFb animal retina Fibroblast CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA |
| title_short |
FGFb E O CRESCIMENTO DE PROCESSOS DAS CÉLULAS DA RETINA |
| title_full |
FGFb E O CRESCIMENTO DE PROCESSOS DAS CÉLULAS DA RETINA |
| title_fullStr |
FGFb E O CRESCIMENTO DE PROCESSOS DAS CÉLULAS DA RETINA |
| title_full_unstemmed |
FGFb E O CRESCIMENTO DE PROCESSOS DAS CÉLULAS DA RETINA |
| title_sort |
FGFb E O CRESCIMENTO DE PROCESSOS DAS CÉLULAS DA RETINA |
| author |
Souza, Andreza Rozendo de |
| author_facet |
Souza, Andreza Rozendo de |
| author_role |
author |
| dc.contributor.none.fl_str_mv |
Araújo, Elizabeth Giestal de CPF:55544433122 http://lattes.cnpq.br/8230334072312477 |
| dc.contributor.author.fl_str_mv |
Souza, Andreza Rozendo de |
| dc.subject.por.fl_str_mv |
Sistema nervoso Desenvolvimento da retina FGFb retina animal Fibroblasto Nervous system, development of the retina FGFb animal retina Fibroblast CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA |
| topic |
Sistema nervoso Desenvolvimento da retina FGFb retina animal Fibroblasto Nervous system, development of the retina FGFb animal retina Fibroblast CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA |
| description |
Basic fibroblast growth factor plays an important role during the development of the nervous system mediating different events such as proliferation, migration, survival and neurite outgrowth. The aim of this work was to analyze the effect of basic fibroblast growth factor on the outgrowth of processes from neonatal retinal cells kept in culture. We also investigated the intracellular pathways involved in this effect. Our results clearly show that 25ng/mL bFGF increases the amount of cells with process greater than cell soma but, the total number of the cells was not affected. The inhibitors of MEK (50μM PD98059), PI3-kinase (25μM LY294002), PKC (1,25μM queleritrine) and Src (1,0μM PP1) blocked completely the effect of bFGF whereas the inhibitors of epidermal growth factor receptor (2,5μM tyrfostin), L type Ca2+ channel (5,0μM nifedipine) and intracellular Ca 2+ chelator (20μM BAPTA-AM) were not able to blocky the effect of bFGF. An interesting effect was observed when cells were treated with a p38 inhibitor (20μM SB 202190). This drug induced an increase in process outgrowth and when incubated with bFGF we observed a sinergistic effect. Our results indicate that MEK, PI3-kinase, PKC and Src play an important role in the effect of FGFb on process outgrowth from retinal cells. |
| publishDate |
2009 |
| dc.date.none.fl_str_mv |
2009-07-27 2021-03-10T20:44:39Z 2021-03-10T20:44:39Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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https://app.uff.br/riuff/handle/1/18463 |
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ark:/87559/0013000002wvq |
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https://app.uff.br/riuff/handle/1/18463 |
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ark:/87559/0013000002wvq |
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por |
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por |
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CC-BY-SA info:eu-repo/semantics/openAccess |
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CC-BY-SA |
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openAccess |
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application/pdf |
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Programa de Pós-graduação em Neuroimunologia Neuroimunologia |
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Programa de Pós-graduação em Neuroimunologia Neuroimunologia |
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reponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF) instname:Universidade Federal Fluminense (UFF) instacron:UFF |
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Universidade Federal Fluminense (UFF) |
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UFF |
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Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
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Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
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Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF) |
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riuff@id.uff.br |
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