Processos fermentativos em res?duos agroindustriais utilizando a levedura yarrowia lipolytica qu69

Detalhes bibliográficos
Ano de defesa: 2018
Autor(a) principal: Souza, Fernanda Arpini
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal da Fronteira Sul
Brasil
Campus Laranjeiras do Sul
Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos
UFFS
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Res?duos s?lidos
Tratamento de res?duos
Processos qu?micos
Leveduras
Link de acesso: https://rd.uffs.edu.br/handle/prefix/2110
Resumo: The constant development of biotechnology and the search for processes in which it is possible to add commercial value to previously discarded products, the possibility arises of the use of agroindustrial residues in bioconversion processes. These processes are performed by microorganisms and have the advantages of low cost, easy control and non-use of toxic chemicals. The Yeast such, Yarrowia lipolytica was used in bioconversion processes for the production of various products of industrial interest. To estimate the number of cells present in the inoculum that will be used in fermentative processes, the two most used methods are the McFarland scale and the Neubauer chamber, which were originally developed for counting bacteria and blood cells, respectively, subjective. In order to improve the yeast cell counting methods Yarrowia lipolytica QU69, was use in bioprocesses the McFarland scale associated with UV-Vis spectrophotometry and used to estimate the number of cells present in suspensions. The method proved to be very fast, reliable and reproducible, and could be applied in routine analyzes and classroom experiments. In the bioconversion process using yeast cells Yarrowia lipolytica QU69 the biomass produced was evaluated by the production of proteins and lipids with several carbon sources from agroindustrial waste and the process was optimized. Significant values were obtained when using manioc as a carbon source, urea-containing supplementation medium and a process conducted under dubnoff shaking at 100 rpm and a temperature of 40 ? C.
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spelling Processos fermentativos em res?duos agroindustriais utilizando a levedura yarrowia lipolytica qu69Res?duos s?lidosTratamento de res?duosProcessos qu?micosLevedurasThe constant development of biotechnology and the search for processes in which it is possible to add commercial value to previously discarded products, the possibility arises of the use of agroindustrial residues in bioconversion processes. These processes are performed by microorganisms and have the advantages of low cost, easy control and non-use of toxic chemicals. The Yeast such, Yarrowia lipolytica was used in bioconversion processes for the production of various products of industrial interest. To estimate the number of cells present in the inoculum that will be used in fermentative processes, the two most used methods are the McFarland scale and the Neubauer chamber, which were originally developed for counting bacteria and blood cells, respectively, subjective. In order to improve the yeast cell counting methods Yarrowia lipolytica QU69, was use in bioprocesses the McFarland scale associated with UV-Vis spectrophotometry and used to estimate the number of cells present in suspensions. The method proved to be very fast, reliable and reproducible, and could be applied in routine analyzes and classroom experiments. In the bioconversion process using yeast cells Yarrowia lipolytica QU69 the biomass produced was evaluated by the production of proteins and lipids with several carbon sources from agroindustrial waste and the process was optimized. Significant values were obtained when using manioc as a carbon source, urea-containing supplementation medium and a process conducted under dubnoff shaking at 100 rpm and a temperature of 40 ? C.Diante do desenvolvimento constante da biotecnologia e a busca por processos em que seja poss?vel agregar valor comercial a produtos at? ent?o descartados, surge a possibilidade da utiliza??o de res?duos agroindustriais em processos de bioconvers?o. Estes processos s?o realizados por micro-organismos e possuem como vantagens o baixo custo, f?cil controle e a n?o utiliza??o de produtos qu?micos. A levedura Yarrowia lipolytica ? utilizada em processos de bioconvers?o para produ??o de diversos produtos de interesse industrial. Para estimar o n?mero de c?lulas presentes nos in?culos que ser?o utilizados em processos fermentativos os dois m?todos mais utilizados s?o a escala de McFarland e a c?mara de Neubauer, que foram originalmente desenvolvidos para contagem de bact?rias e c?lulas sangu?neas, respectivamente, o m?todos citados possuem interpreta??o subjetiva. Buscando aperfei?oar os m?todos de contagem de c?lulas da levedura Yarrowia lipolytica QU69, para utiliza??o em bioprocessos foi utilizada a escala de McFarland associada ? espectrofotometria UV-Vis para estimar o n?mero de c?lulas presentes em suspens?es. O m?todo se mostrou bastante r?pido, confi?vel e reprodut?vel, podendo ser aplicado em an?lises de rotina e experimentos de aulas. No processo de bioconvers?o utilizando as c?lulas da levedura Yarrowia lipolytica QU69 a biomassa produzida foi avaliada quanto a produ??o de prote?nas e lip?dios com diversas fontes de carbono oriundas de res?duos agroindustriais e buscou-se a otimiza??o do processo. Foram alcan?ados valores expressivos quando utilizado res?duo de casca de mandioca como fonte de carbono, meio de suplementa??o contendo ur?ia e processo conduzido sob agita??o tipo dubnoff a 100 rpm e temperatura de 40?C.Universidade Federal da Fronteira SulBrasilCampus Laranjeiras do SulPrograma de P?s-Gradua??o em Ci?ncia e Tecnologia de AlimentosUFFSBitencourt, Thiago BerglerSouza, Fernanda Arpini20182018-09-12T12:45:45Z2018-09-122018-09-12T12:45:45Z2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttps://rd.uffs.edu.br/handle/prefix/2110porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFFS (Repositório Digital da UFFS)instname:Universidade Federal Fronteira do Sul (UFFS)instacron:UFFS2021-09-29T13:21:46Zoai:rd.uffs.edu.br:prefix/2110Repositório InstitucionalPUBhttps://rd.uffs.edu.br/oai/requestfranciele.cruz@uffs.edu.bropendoar:39242021-09-29T13:21:46Repositório Institucional da UFFS (Repositório Digital da UFFS) - Universidade Federal Fronteira do Sul (UFFS)false
dc.title.none.fl_str_mv Processos fermentativos em res?duos agroindustriais utilizando a levedura yarrowia lipolytica qu69
title Processos fermentativos em res?duos agroindustriais utilizando a levedura yarrowia lipolytica qu69
spellingShingle Processos fermentativos em res?duos agroindustriais utilizando a levedura yarrowia lipolytica qu69
Souza, Fernanda Arpini
Res?duos s?lidos
Tratamento de res?duos
Processos qu?micos
Leveduras
title_short Processos fermentativos em res?duos agroindustriais utilizando a levedura yarrowia lipolytica qu69
title_full Processos fermentativos em res?duos agroindustriais utilizando a levedura yarrowia lipolytica qu69
title_fullStr Processos fermentativos em res?duos agroindustriais utilizando a levedura yarrowia lipolytica qu69
title_full_unstemmed Processos fermentativos em res?duos agroindustriais utilizando a levedura yarrowia lipolytica qu69
title_sort Processos fermentativos em res?duos agroindustriais utilizando a levedura yarrowia lipolytica qu69
author Souza, Fernanda Arpini
author_facet Souza, Fernanda Arpini
author_role author
dc.contributor.none.fl_str_mv Bitencourt, Thiago Bergler
dc.contributor.author.fl_str_mv Souza, Fernanda Arpini
dc.subject.por.fl_str_mv Res?duos s?lidos
Tratamento de res?duos
Processos qu?micos
Leveduras
topic Res?duos s?lidos
Tratamento de res?duos
Processos qu?micos
Leveduras
description The constant development of biotechnology and the search for processes in which it is possible to add commercial value to previously discarded products, the possibility arises of the use of agroindustrial residues in bioconversion processes. These processes are performed by microorganisms and have the advantages of low cost, easy control and non-use of toxic chemicals. The Yeast such, Yarrowia lipolytica was used in bioconversion processes for the production of various products of industrial interest. To estimate the number of cells present in the inoculum that will be used in fermentative processes, the two most used methods are the McFarland scale and the Neubauer chamber, which were originally developed for counting bacteria and blood cells, respectively, subjective. In order to improve the yeast cell counting methods Yarrowia lipolytica QU69, was use in bioprocesses the McFarland scale associated with UV-Vis spectrophotometry and used to estimate the number of cells present in suspensions. The method proved to be very fast, reliable and reproducible, and could be applied in routine analyzes and classroom experiments. In the bioconversion process using yeast cells Yarrowia lipolytica QU69 the biomass produced was evaluated by the production of proteins and lipids with several carbon sources from agroindustrial waste and the process was optimized. Significant values were obtained when using manioc as a carbon source, urea-containing supplementation medium and a process conducted under dubnoff shaking at 100 rpm and a temperature of 40 ? C.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-09-12T12:45:45Z
2018-09-12
2018-09-12T12:45:45Z
2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rd.uffs.edu.br/handle/prefix/2110
url https://rd.uffs.edu.br/handle/prefix/2110
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal da Fronteira Sul
Brasil
Campus Laranjeiras do Sul
Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos
UFFS
publisher.none.fl_str_mv Universidade Federal da Fronteira Sul
Brasil
Campus Laranjeiras do Sul
Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos
UFFS
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFFS (Repositório Digital da UFFS)
instname:Universidade Federal Fronteira do Sul (UFFS)
instacron:UFFS
instname_str Universidade Federal Fronteira do Sul (UFFS)
instacron_str UFFS
institution UFFS
reponame_str Repositório Institucional da UFFS (Repositório Digital da UFFS)
collection Repositório Institucional da UFFS (Repositório Digital da UFFS)
repository.name.fl_str_mv Repositório Institucional da UFFS (Repositório Digital da UFFS) - Universidade Federal Fronteira do Sul (UFFS)
repository.mail.fl_str_mv franciele.cruz@uffs.edu.br
_version_ 1868248298991648768

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