Prospecção de peptídeos derivados das peçonhas de animais da Região Centro Oeste com atividade antimicobacteriana

Detalhes bibliográficos
Ano de defesa: 2015
Autor(a) principal: Neves, Rogério Coutinho das lattes
Orientador(a): Junqueira-Kipnis, Ana Paula lattes
Banca de defesa: Cardoso, Juliana Lamaro, Vieira, José Daniel Gonçalves, Junqueira-Kipnis, Ana Paula
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Goiás
Programa de Pós-Graduação: Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)
Departamento: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
País: Brasil
Palavras-chave em Português:
Mycobacterium abscessus subsp. bolletii
Peptídeo antimicrobiano
Micobactéria atípica
Palavras-chave em Inglês:
Mycobacterium abscessus subsp. bolletii
Antimicrobial peptide
Atypical mycobacteria
Área do conhecimento CNPq:
SAUDE COLETIVA::SAUDE PUBLICA
Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/5584
Resumo: Outbreaks caused by atypical mycobacteria are considered as an emerging problem in Brazil and Goias, being frequently associated with invasive procedures, such as laparoscopy, arthroscopy, and aesthetic surgeries among others. Treatment of mycobacteriosis requires highly toxic drugs with doubtful sterilizing effects. Consequently, several studies aim to search for new drugs or molecules with anti-mycobacterial biological activities. The objective of this study was to prospect biomolecules from the venom of the social wasp Polybia paulista (Vespoidea, Vespidae, Polistinae) and from the Tityus sp scorpion with antimycobacteria activity against Mycobacterium abscessus subsp. bolletii (GO06). Peptides from the wasp and scorpion venoms were evaluated in broth microdilution assay and in macrophage infected cultures to determine minimum inhibitory concentration. Peptides with antimycobacterial activity were further evaluated by scanning electron microscopy of M. abscessus subsp. bolletii treated cultures. The peptide toxicities were evaluated on macrophage cultures. Peptides Agelaia-MP, Polybia-MPII, and AVA, derived from wasp and peptides AMP 1, 2, 4, 6, and 8 derived from scorpion presented antimycobacterial activities. AVA and AMP 4 peptides were not toxic for macrophages. In this work three peptides derived from the wasp venom and five peptides derived from the scorpion venom were identified as having antimycobacterial activity.
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spelling Junqueira-Kipnis, Ana Paulahttp://lattes.cnpq.br/1252262903952987Kipnis, Andréhttp://lattes.cnpq.br/4434965360286741Cardoso, Juliana LamaroVieira, José Daniel GonçalvesJunqueira-Kipnis, Ana Paulahttp://lattes.cnpq.br/8544101569861271Neves, Rogério Coutinho das2016-05-20T11:12:51Z2015-02-27NEVES, R. C. Prospecção de peptídeos derivados das peçonhas de animais da Região Centro Oeste com atividade antimicobacteriana. 2015. 97 f. Dissertação (Mestrado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2015.http://repositorio.bc.ufg.br/tede/handle/tede/5584Outbreaks caused by atypical mycobacteria are considered as an emerging problem in Brazil and Goias, being frequently associated with invasive procedures, such as laparoscopy, arthroscopy, and aesthetic surgeries among others. Treatment of mycobacteriosis requires highly toxic drugs with doubtful sterilizing effects. Consequently, several studies aim to search for new drugs or molecules with anti-mycobacterial biological activities. The objective of this study was to prospect biomolecules from the venom of the social wasp Polybia paulista (Vespoidea, Vespidae, Polistinae) and from the Tityus sp scorpion with antimycobacteria activity against Mycobacterium abscessus subsp. bolletii (GO06). Peptides from the wasp and scorpion venoms were evaluated in broth microdilution assay and in macrophage infected cultures to determine minimum inhibitory concentration. Peptides with antimycobacterial activity were further evaluated by scanning electron microscopy of M. abscessus subsp. bolletii treated cultures. The peptide toxicities were evaluated on macrophage cultures. Peptides Agelaia-MP, Polybia-MPII, and AVA, derived from wasp and peptides AMP 1, 2, 4, 6, and 8 derived from scorpion presented antimycobacterial activities. AVA and AMP 4 peptides were not toxic for macrophages. In this work three peptides derived from the wasp venom and five peptides derived from the scorpion venom were identified as having antimycobacterial activity.Surtos causados por micobactérias atípicas são considerados emergentes no Brasil e em Goiás e têm tem sido associados a procedimentos invasivos, como a laparoscopia, artroscopia e cirurgia estética dentre outros. O tratamento de micobacterioses envolve drogas altamente tóxicas que apresentam efeito esterilizante relativo. Portanto, vários estudos têm como alvo a busca de novas drogas ou moléculas biológicas com atividade micobactericida. O objetivo deste estudo foi prospectar biomoléculas do veneno de vespa social Polybia paulista (Vespoidea, Vespidae, Polistinae) e de escorpiões do gênero Tityus com atividade micobactericida in vitro contra Mycobacterium abscessus subsp. bolletii (GO06). Peptídeos derivados do veneno da vespa e do escorpião foram avaliados por microdiluição em caldo, e em culturas de macrófagos infectados determinando a concentração inibitória mínima. Para os peptídeos com atividade micobactericida, o potencial dano causado à parede celular da micobactéria foi analisado por microscopia eletrônica de varredura. A toxicidade de dois peptídeos com potencial uso farmacológico foram avaliados em culturas de macrófagos peritoneais de camundongos BALB/c. Os peptídeos Agelaia-MP, Polybia-MPII e AVA derivados do veneno da vespa e os peptídeos AMP 1, 2, 4, 6 e 8 derivados do veneno de escorpião apresentaram atividade micobactericida. Os peptídeos micobactericidas agem desestruturando os envoltório das bacilos de M. abscessos subsp. bolletti. Os peptídeos AVA e o AMP 4 não apresentaram toxicidade para culturas de macrófagos, e reduziram a carga bacilar de culturas de macrófagos infectadas com M. abscessus subsp. bolletii. Neste trabalho identificou-se três peptídeos derivados da peçonha de vespa e cinco peptídeos derivados de veneno de escorpião com atividade micobactericida.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2016-05-20T11:06:25Z No. of bitstreams: 2 Dissertação - Rogerio Coutinho das Neves - 2015.pdf: 6048850 bytes, checksum: 1bac6d1d4aa28a3cf0ebbb228b593986 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-05-20T11:12:51Z (GMT) No. of bitstreams: 2 Dissertação - Rogerio Coutinho das Neves - 2015.pdf: 6048850 bytes, checksum: 1bac6d1d4aa28a3cf0ebbb228b593986 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2016-05-20T11:12:51Z (GMT). No. of bitstreams: 2 Dissertação - Rogerio Coutinho das Neves - 2015.pdf: 6048850 bytes, checksum: 1bac6d1d4aa28a3cf0ebbb228b593986 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2015-02-27Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)UFGBrasilInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessMycobacterium abscessus subsp. bolletiiPeptídeo antimicrobianoMicobactéria atípicaMycobacterium abscessus subsp. bolletiiAntimicrobial peptideAtypical mycobacteriaSAUDE COLETIVA::SAUDE PUBLICAProspecção de peptídeos derivados das peçonhas de animais da Região Centro Oeste com atividade antimicobacterianaProspecting of peptides derived from the west region animal venoms with antimycobacterial activityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis6085308344741430434600600600600-77690114445645562883112649244584600979-2555911436985713659reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv Prospecção de peptídeos derivados das peçonhas de animais da Região Centro Oeste com atividade antimicobacteriana
dc.title.alternative.eng.fl_str_mv Prospecting of peptides derived from the west region animal venoms with antimycobacterial activity
title Prospecção de peptídeos derivados das peçonhas de animais da Região Centro Oeste com atividade antimicobacteriana
spellingShingle Prospecção de peptídeos derivados das peçonhas de animais da Região Centro Oeste com atividade antimicobacteriana
Neves, Rogério Coutinho das
Mycobacterium abscessus subsp. bolletii
Peptídeo antimicrobiano
Micobactéria atípica
Mycobacterium abscessus subsp. bolletii
Antimicrobial peptide
Atypical mycobacteria
SAUDE COLETIVA::SAUDE PUBLICA
title_short Prospecção de peptídeos derivados das peçonhas de animais da Região Centro Oeste com atividade antimicobacteriana
title_full Prospecção de peptídeos derivados das peçonhas de animais da Região Centro Oeste com atividade antimicobacteriana
title_fullStr Prospecção de peptídeos derivados das peçonhas de animais da Região Centro Oeste com atividade antimicobacteriana
title_full_unstemmed Prospecção de peptídeos derivados das peçonhas de animais da Região Centro Oeste com atividade antimicobacteriana
title_sort Prospecção de peptídeos derivados das peçonhas de animais da Região Centro Oeste com atividade antimicobacteriana
author Neves, Rogério Coutinho das
author_facet Neves, Rogério Coutinho das
author_role author
dc.contributor.advisor1.fl_str_mv Junqueira-Kipnis, Ana Paula
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/1252262903952987
dc.contributor.advisor-co1.fl_str_mv Kipnis, André
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/4434965360286741
dc.contributor.referee1.fl_str_mv Cardoso, Juliana Lamaro
dc.contributor.referee2.fl_str_mv Vieira, José Daniel Gonçalves
dc.contributor.referee3.fl_str_mv Junqueira-Kipnis, Ana Paula
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/8544101569861271
dc.contributor.author.fl_str_mv Neves, Rogério Coutinho das
contributor_str_mv Junqueira-Kipnis, Ana Paula
Kipnis, André
Cardoso, Juliana Lamaro
Vieira, José Daniel Gonçalves
Junqueira-Kipnis, Ana Paula
dc.subject.por.fl_str_mv Mycobacterium abscessus subsp. bolletii
Peptídeo antimicrobiano
Micobactéria atípica
topic Mycobacterium abscessus subsp. bolletii
Peptídeo antimicrobiano
Micobactéria atípica
Mycobacterium abscessus subsp. bolletii
Antimicrobial peptide
Atypical mycobacteria
SAUDE COLETIVA::SAUDE PUBLICA
dc.subject.eng.fl_str_mv Mycobacterium abscessus subsp. bolletii
Antimicrobial peptide
Atypical mycobacteria
dc.subject.cnpq.fl_str_mv SAUDE COLETIVA::SAUDE PUBLICA
description Outbreaks caused by atypical mycobacteria are considered as an emerging problem in Brazil and Goias, being frequently associated with invasive procedures, such as laparoscopy, arthroscopy, and aesthetic surgeries among others. Treatment of mycobacteriosis requires highly toxic drugs with doubtful sterilizing effects. Consequently, several studies aim to search for new drugs or molecules with anti-mycobacterial biological activities. The objective of this study was to prospect biomolecules from the venom of the social wasp Polybia paulista (Vespoidea, Vespidae, Polistinae) and from the Tityus sp scorpion with antimycobacteria activity against Mycobacterium abscessus subsp. bolletii (GO06). Peptides from the wasp and scorpion venoms were evaluated in broth microdilution assay and in macrophage infected cultures to determine minimum inhibitory concentration. Peptides with antimycobacterial activity were further evaluated by scanning electron microscopy of M. abscessus subsp. bolletii treated cultures. The peptide toxicities were evaluated on macrophage cultures. Peptides Agelaia-MP, Polybia-MPII, and AVA, derived from wasp and peptides AMP 1, 2, 4, 6, and 8 derived from scorpion presented antimycobacterial activities. AVA and AMP 4 peptides were not toxic for macrophages. In this work three peptides derived from the wasp venom and five peptides derived from the scorpion venom were identified as having antimycobacterial activity.
publishDate 2015
dc.date.issued.fl_str_mv 2015-02-27
dc.date.accessioned.fl_str_mv 2016-05-20T11:12:51Z
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dc.identifier.citation.fl_str_mv NEVES, R. C. Prospecção de peptídeos derivados das peçonhas de animais da Região Centro Oeste com atividade antimicobacteriana. 2015. 97 f. Dissertação (Mestrado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2015.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/5584
identifier_str_mv NEVES, R. C. Prospecção de peptídeos derivados das peçonhas de animais da Região Centro Oeste com atividade antimicobacteriana. 2015. 97 f. Dissertação (Mestrado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2015.
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publisher.none.fl_str_mv Universidade Federal de Goiás
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