Avaliação da densidade folicular e de estratégias para otimizar a obtenção de folículos pré-antrais equinos

• Ano de defesa: 2014
• Autor(a) principal: Alves, Kele Amaral
• Orientador(a): Gambarini, Maria Lúcia
• Banca de defesa: Meirinhos, Maria Lúcia Gambarini, Gastal, Eduardo Leite, Oña, Cely Marini Melo e, Oliveira, Rodrigo Arruda de, Porto, Regiani Nascimento Gagno
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• dARK ID: ark:/38995/0013000008j49
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Ciência Animal (EVZ)
• Departamento: Escola de Veterinária e Zootecnia - EVZ (RG)
• País: Brasil
• Palavras-chave em Português: Biópsia ovariana; Densidade folicular; Égua; Folículos pré-antrais
• Palavras-chave em Inglês: Follicular density; Mare; Ovarian biopsy; Preantral follicles
• Área do conhecimento CNPq: MEDICINA VETERINARIA::REPRODUCAO ANIMAL
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/5118

Studies with equine preantral follicles are scarce and the follicular dynamics features remain unclear. The mare’s ovarian function (e.g., folliculogenesis) has similarities with woman’s cycle and based on these aspects, this species can be considered an animal model for comparative studies and techniques with preantral follicles to preserve female fertility. The purpose of the first project was to determine methodologies that might speed up the process of histological evaluation of preantral follicle population. On the second project, we evaluated the follicular population features considering the follicular density factor by means of a mathematical model, to determine the appropriate experimental design to estimate the follicular density in ovarian fragments. The third project, analyzed the effects of reproductive phases, ovary, and ovarian structures on the quality, classification, follicular and cellular densities in ovarian fragments. The results allowed an establishment of the best thickness of histological sections and the ideal number of fragments and sections to estimate the equine preantral follicle density. Furthermore, it was observed a low follicular density associated with a high heterogeneity within and among animals. Moreover, we demonstrated the best scenario (diestrous phase with the presence of a functional corpus luteum) to collect ovarian fragments with higher follicular and cellular densities. In summary, this study demonstrated: (i) advance on the knowledge of equine follicular dynamic on the preantral phase; (ii) the concept of the mare as an animal model to study the reproductive challenges on woman; and (iii) provided basic information for future biotechnology research with the aim to preserve or improve the reproductive capability as cryopreservation, in vitro culture, and transplants of ovarian tissue.