Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis
| Ano de defesa: | 2011 |
|---|---|
| Autor(a) principal: |
Philippsen, Hellen Kempfer
 |
| Orientador(a): |
Soares, Célia Maria de Almeida
|
| Banca de defesa: | , , , , |
| Tipo de documento: | Dissertação |
| Tipo de acesso: | Acesso aberto |
| Idioma: | por |
| Instituição de defesa: |
Universidade Federal de Goiás
|
| Programa de Pós-Graduação: |
Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)
|
| Departamento: |
Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
|
| País: |
Brasil
|
| Palavras-chave em Português: | |
| Palavras-chave em Inglês: | |
| Área do conhecimento CNPq: | |
| Link de acesso: | http://repositorio.bc.ufg.br/tede/handle/tede/3222 |
Resumo: | Paracoccidioides brasiliensis is a dimorphic fungus, the causative agent of paracoccidioidomycosis (PCM), a systemic mycosis that affects mainly people from Latin America. In order to establish infection, many factors are required for P. brasiliensis, such as cell wall remodeling and the ability to capture nutrients from the host. As iron is an important cofactor of many reactions, the host reduces the availability of free iron ions to the pathogen. Therefore, this study aims to elucidate the proteomic profile of the cell wall in response to iron depletion. The extraction of surface proteins of the cell wall in iron restriction and iron replete conditions was performed and the proteomic profile was obtained by two-dimensional electrophoresis with subsequent analysis of images. Statistical analysis demonstrated proteins with differential level of expression in each condition, which were subjected to tryptic digestion and identification by mass spectrometry. It was identified 26 proteins by peptide mass fingerprint (PMF) and/or ions fragmentation (MS/MS). The majority of these proteins have post - translational modifications and only some have in their sequences signal peptide, suggesting that they are addressed to the surface by non-classical pathways. |
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UFG-2_30e676c2022de26b73df8d5e52542158 |
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oai:repositorio.bc.ufg.br:tede/3222 |
| network_acronym_str |
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| network_name_str |
Repositório Institucional da UFG |
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Soares, Célia Maria de Almeidahttp://lattes.cnpq.br/8539946335852637Soares, Célia Maria de AlmeidaKipnis, AndréPereira, MaristelaBailão, Alexandre MeloBorges, Clayton Luíshttp://lattes.cnpq.br/9963999800396051Philippsen, Hellen Kempfer2014-09-30T15:28:08Z2011-08-30PHILIPPSEN, Hellen Kempfer. Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis. 2011. 57 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2011.http://repositorio.bc.ufg.br/tede/handle/tede/3222Paracoccidioides brasiliensis is a dimorphic fungus, the causative agent of paracoccidioidomycosis (PCM), a systemic mycosis that affects mainly people from Latin America. In order to establish infection, many factors are required for P. brasiliensis, such as cell wall remodeling and the ability to capture nutrients from the host. As iron is an important cofactor of many reactions, the host reduces the availability of free iron ions to the pathogen. Therefore, this study aims to elucidate the proteomic profile of the cell wall in response to iron depletion. The extraction of surface proteins of the cell wall in iron restriction and iron replete conditions was performed and the proteomic profile was obtained by two-dimensional electrophoresis with subsequent analysis of images. Statistical analysis demonstrated proteins with differential level of expression in each condition, which were subjected to tryptic digestion and identification by mass spectrometry. It was identified 26 proteins by peptide mass fingerprint (PMF) and/or ions fragmentation (MS/MS). The majority of these proteins have post - translational modifications and only some have in their sequences signal peptide, suggesting that they are addressed to the surface by non-classical pathways.Paracoccidioides brasiliensis é um fungo patogênico termodimórfico, agente causador da Paracoccidioidomicose (PCM), uma micose sistêmica que afeta principalmente indivíduos da América Latina. Muitos fatores são necessários para que P. brasiliensis estabeleça infecção, como o remodelamento da parede celular e a capacidade de captar nutrientes do hospedeiro para seu metabolismo. Como o íon ferro é um cofator de diversas reações, o hospedeiro diminui a disponibilidade de íons ferro na forma livre para o patógeno. Portanto, o presente trabalho visa investigar o perfil de proteínas da parede celular de P. brasiliensis quando em condição de depleção do íon ferro. Para esse fim, realizou-se a extração das proteínas da parede das células de P. brasiliensis em meio com restrição do íon ferro. O perfil proteômico das condições experimental e controle foram obtidos por eletroforese bidimensional com posterior análise de imagens. Análises estatísticas demonstraram as proteínas reguladas nas condições de depleção de ferro, que foram submetidas à digestão tríptica e identificação por espectrometria de massas. Foram identificadas 26 proteínas por perfil de digestão tríptica (PMF) e/ou perfil de fragmentação dos íons (MS/MS). A maioria dessas proteínas apresenta preditas modificações pós - traducionais e apenas algumas apresentam peptídeo sinal em suas sequências, o que sugere que elas são endereçadas à superfície por vias não clássicas.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2014-09-30T12:45:52Z No. of bitstreams: 2 Philippsen, Hellen Kempfer-2011-dissertação.pdf: 1457910 bytes, checksum: 4ee585b7a28a164d44414a0a2ef76ae5 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2014-09-30T15:28:08Z (GMT) No. of bitstreams: 2 Philippsen, Hellen Kempfer-2011-dissertação.pdf: 1457910 bytes, checksum: 4ee585b7a28a164d44414a0a2ef76ae5 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2014-09-30T15:28:08Z (GMT). No. of bitstreams: 2 Philippsen, Hellen Kempfer-2011-dissertação.pdf: 1457910 bytes, checksum: 4ee585b7a28a164d44414a0a2ef76ae5 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2011-08-30Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfhttp://repositorio.bc.ufg.br/tede/retrieve/9102/Philippsen%2c%20Hellen%20Kempfer-2011-disserta%c3%a7%c3%a3o.pdf.jpgporUniversidade Federal de GoiásPrograma de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)UFGBrasilInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)1. Abdul, R.A.; Oellerich, M.; Amstrong, V.W.; Riemenschneider, B.; Reichard, U. (2006). Proteome of conidial surface associated proteins of Aspergillus fumigatus reflecting potential vaccine candidates and allergens. Proteome research. 5, 954-962. 2. Almeida, F.B.D.R.; Carvalho, F.C.; Mariano, V.S.; Alegre, A.C.P.; Silva, R.N.; Hanna, E.S.; Roque-Barreira, M.C. (2011). 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Proteome Science 6, 336085308344741430434600600600600-77690114445645562888648187577661350585-2555911436985713659http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessParacoccidioides brasiliensisParede celularParacoccidioidomicoseDeficiência de ferroIronCell wallProteinMICROBIOLOGIA APLICADA::MICROBIOLOGIA MEDICAInfluência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensisInfluence of iron in the composition of surface protein non-covalently bound of paracoccidioides brasiliensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisreponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://repositorio.bc.ufg.br/tede/bitstreams/1b44e3fb-2ddc-4db4-9b87-e07e3d18ab4e/downloadbd3efa91386c1718a7f26a329fdcb468MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.bc.ufg.br/tede/bitstreams/655243e1-69d9-4271-879e-40cb59c721d4/download4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; 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| dc.title.por.fl_str_mv |
Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis |
| dc.title.alternative.eng.fl_str_mv |
Influence of iron in the composition of surface protein non-covalently bound of paracoccidioides brasiliensis |
| title |
Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis |
| spellingShingle |
Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis Philippsen, Hellen Kempfer Paracoccidioides brasiliensis Parede celular Paracoccidioidomicose Deficiência de ferro Iron Cell wall Protein MICROBIOLOGIA APLICADA::MICROBIOLOGIA MEDICA |
| title_short |
Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis |
| title_full |
Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis |
| title_fullStr |
Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis |
| title_full_unstemmed |
Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis |
| title_sort |
Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis |
| author |
Philippsen, Hellen Kempfer |
| author_facet |
Philippsen, Hellen Kempfer |
| author_role |
author |
| dc.contributor.advisor1.fl_str_mv |
Soares, Célia Maria de Almeida |
| dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8539946335852637 |
| dc.contributor.referee1.fl_str_mv |
Soares, Célia Maria de Almeida |
| dc.contributor.referee2.fl_str_mv |
Kipnis, André |
| dc.contributor.referee3.fl_str_mv |
Pereira, Maristela |
| dc.contributor.referee4.fl_str_mv |
Bailão, Alexandre Melo |
| dc.contributor.referee5.fl_str_mv |
Borges, Clayton Luís |
| dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9963999800396051 |
| dc.contributor.author.fl_str_mv |
Philippsen, Hellen Kempfer |
| contributor_str_mv |
Soares, Célia Maria de Almeida Soares, Célia Maria de Almeida Kipnis, André Pereira, Maristela Bailão, Alexandre Melo Borges, Clayton Luís |
| dc.subject.por.fl_str_mv |
Paracoccidioides brasiliensis Parede celular Paracoccidioidomicose Deficiência de ferro |
| topic |
Paracoccidioides brasiliensis Parede celular Paracoccidioidomicose Deficiência de ferro Iron Cell wall Protein MICROBIOLOGIA APLICADA::MICROBIOLOGIA MEDICA |
| dc.subject.eng.fl_str_mv |
Iron Cell wall Protein |
| dc.subject.cnpq.fl_str_mv |
MICROBIOLOGIA APLICADA::MICROBIOLOGIA MEDICA |
| description |
Paracoccidioides brasiliensis is a dimorphic fungus, the causative agent of paracoccidioidomycosis (PCM), a systemic mycosis that affects mainly people from Latin America. In order to establish infection, many factors are required for P. brasiliensis, such as cell wall remodeling and the ability to capture nutrients from the host. As iron is an important cofactor of many reactions, the host reduces the availability of free iron ions to the pathogen. Therefore, this study aims to elucidate the proteomic profile of the cell wall in response to iron depletion. The extraction of surface proteins of the cell wall in iron restriction and iron replete conditions was performed and the proteomic profile was obtained by two-dimensional electrophoresis with subsequent analysis of images. Statistical analysis demonstrated proteins with differential level of expression in each condition, which were subjected to tryptic digestion and identification by mass spectrometry. It was identified 26 proteins by peptide mass fingerprint (PMF) and/or ions fragmentation (MS/MS). The majority of these proteins have post - translational modifications and only some have in their sequences signal peptide, suggesting that they are addressed to the surface by non-classical pathways. |
| publishDate |
2011 |
| dc.date.issued.fl_str_mv |
2011-08-30 |
| dc.date.accessioned.fl_str_mv |
2014-09-30T15:28:08Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| format |
masterThesis |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
PHILIPPSEN, Hellen Kempfer. Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis. 2011. 57 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2011. |
| dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/3222 |
| identifier_str_mv |
PHILIPPSEN, Hellen Kempfer. Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis. 2011. 57 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2011. |
| url |
http://repositorio.bc.ufg.br/tede/handle/tede/3222 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.relation.program.fl_str_mv |
6085308344741430434 |
| dc.relation.confidence.fl_str_mv |
600 600 600 600 |
| dc.relation.department.fl_str_mv |
-7769011444564556288 |
| dc.relation.cnpq.fl_str_mv |
8648187577661350585 |
| dc.relation.sponsorship.fl_str_mv |
-2555911436985713659 |
| dc.relation.references.por.fl_str_mv |
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