Avaliação da ocorrência de Helicobacter pylori em água tratada da bacia do Rio Meia Ponte – Goiânia - GO

• Ano de defesa: 2014
• Autor(a) principal: Carvalho, Ludimila Aparecida Cavalcante Wosnjuk
• Orientador(a): Garciazapata, Marco Tulio Antônio
• Banca de defesa: Garciazapata, Marco Tulio Antonio , Mrué, Fátima, Santos, Sônia de Fátima Oliveira, Silva, Hugo Delleon da
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• dARK ID: ark:/38995/0013000005kv8
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Ciências da Saúde (FM)
• Departamento: Faculdade de Medicina - FM (RG)
• País: Brasil
• Palavras-chave em Português: Helicobacter pylori; Água; Microbiologia ambiental; PCR
• Palavras-chave em Inglês: Helicobacter pylori; Water; Environmental microbiology; PCR.
• Área do conhecimento CNPq: CIENCIAS DA SAUDE
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/7479

Helicobacter pylori is a gran-negative bacteria known as the leading cause of gastritis, peptic ulcer, gastric lymphoma and tissue lymphoma associated to gastric mucosa, which are responsible for almost 9% of cancer deaths in the world. Its mode of transmission is unclear, but it is suggested that the oral-oral, fecal-oral routes in addition to transmission through contaminated food, water, human milk and through animals. Many of these transmission channels related with the water and, therefore, have given importance to develop methodologies capable of detecting bacteria in water samples, since the bacterium in an aquatic environment becomes a state coccoid (known to be viable but not culturable). Thus, the common detection methods based on culture are not as effective in these circumstances. The most widely used techniques in recent years for detecting Helicobacter pylori in water consist of filtration for the concentration of samples, DNA extraction and amplification of genetic material through the Polymerase Chain Reaction (PCR). The study objective was to reproduce these techniques with samples of treated water coming from the public supply of treated water system from the city of Goiânia - Goiás - Brazil. To this end, in partnership with SANEAGO (sanitation company of Goiás), 102 samples of treated water (accounting for 18 points of the distribution network supplied by Meia Ponte River) were collected and analyzed samples for the water temperature , associated with the storage environment, the local climate, the amount of residual chlorine and the presence of Helicobacter pylori DNA in the period from June to November 2013. Those samples were collected during the rainless period, the temperature ranged from 22 ° C, and 33 ° C and the water temperature between 21 ° C and 25 ° C. The residual chlorine was between 0.77 and 1.7. And no positive samples for the presence of DNA of Helicobacter pylori were found. It is therefore concluded the absence of Helicobacter pylori in the samples.