Avaliação de alguns aspectos de toxicidade e eficácia do extrato etanólico de Eugenia dysenterica DC para uso dermocosmético

• Ano de defesa: 2013
• Autor(a) principal: Moreira, Larissa Cleres
• Orientador(a): Valadares, Marize Campos
• Banca de defesa: Valadares, Marize Campos, Lima, Emerson Silva, Taveira, Stephânia Fleury
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Ciências Farmacêuticas (FF)
• Departamento: Faculdade Farmácia - FF (RG)
• País: Brasil
• Palavras-chave em Português: Pele; Cosméticos; Cagaita; Dermatologia; Eugenia dysenterica DC (Myrthaceae); Cuidado e higiene; Fotoproteção
• Palavras-chave em Inglês: Skin; Cosmetics; Dermatology; Fotoprotection
• Área do conhecimento CNPq: CIENCIAS BIOLOGICAS::FARMACOLOGIA
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/3739

The continued growth of dermocosmetics market in recent years has motivated the search for new active compounds for treatment of skin signs. Along with this new reality, awakened desire for natural products in combination with sustainability and authenticity. Eugenia dysenterica DC (Myrthaceae), popularly known as “cagaita”, has antioxidant potential similar to vitamin C and therefore, the present study investigated efficacy of standardized hydroalcoholic extract of E. dysenterica (EED) against aging signs. Inhibition assays collagenase, elastase, and tyrosinase were performed. The sun protection factor was investigated, as well as EED provided photoprotection and cell regeneration in HFF-1 cell line. Also it was investigated the effect of spontaneous melanogenesis and cytotoxicity in B16F10 and B16F0 cells lines and Balb/c 3T3 cell line by the method of reduction of the tetrazolium. It was also studied aspects of toxicity of extract through phototoxicity test by neutral red uptake. Enzyme assays showed a good inhibitory activity of elastase EED, inhibiting it by about 40% at a concentration of 100 µg/mL. For collagenase and tyrosinase, the inhibition can be considered moderate, ± 25% for same concentration. The EED provided an increase of melanin content within ± 20% at a concentration of 30 µg/mL for B16F10 and B16F0. The EED offered no protection against UVB rays, as well as just outlined a tendency to fotoproteger at UVA. For cell regeneration, the extract provided recuperation the cells from 100 µg/mL, so that cell viability reached 107.57 ± 14.92% at concentration of µg/mL. Moreover, it is not phototoxic. Tests for cytotoxicity in 3T3 cells showed an IC50 of 162.3 µg/mL and 100.0 ± µg/mL for B16F10 and B16F0. From data analysis it was concluded that the EED is presented as a promising constituent formulations for dermocosmetic anti-aging, due to its antienzymatic and regenerating action and activity against anti-melanogenesis disorders, as well as being safe as phototoxicity.