Síndrome vestibular em Lithobates catesbeianus associada à ranavirus
Ano de defesa: | 2014 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Goiás
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Programa de Pós-Graduação: |
Programa de Pós-graduação em Ciência Animal (EVZ)
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Departamento: |
Escola de Veterinária e Zootecnia - EVZ (RG)
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País: |
Brasil
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Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.bc.ufg.br/tede/handle/tede/4012 |
Resumo: | Frog farming is an expanding activity in South America. In Brazil it can be considered consolidated, especially in the state ofGoiás. In the last few years it turned into a superintensive activity and consequently favored the emergence of diseases that pose a threat to the technical and economic viability of farms of aquatic organisms. Several diseases with high morbidity and mortality have been observed in tadpoles and frogs. Especially in the state of Goiás, two diseases affect the production cycle in the frog farms. The first manifests itself in 30 days tadpoles leading to high mortality and has been associated to the presence of Ranavirus. The second affects frogs in all stages from post-molt to the point of slaughtering. In post-metamorphic phase the animals are cachectic and 10-50 % of the populationdevelops nervous signs such as incoordination and abnormal postures chronic condition. Despite the board's neurological symptoms, some frogs are able to feed and continue to grow slowly, with few recovering normal condition. This clinical manifestation may be attributed to the anatomy of the vestibular system and hearing. Afferent bilateral vestibular signals from semicircular canal and otolith organs are essential for the stabilization of gaze, the control of posture and locomotion as well as the cognitive aspects of balance, spatial orientation and vegetative homeostasis. Considering the relevance of the topic, the objective of this study is to contribute to the elucidation of Vestibular Syndrome in Rana catesbeiana focusing in the presence of Ranavirus through the use of methods of diagnosis and histopathological techniques , real-time PCR , immunohistochemistry and electron microscopy. In the first study, we developed a method of detecting Ranavirus by real-time PCR for diagnosis of clinically healthy and diseased frogs with nerve signals in order to monitor the spread of this agent for this and other species of animals that inhabit the frog farms around the state of Goiás 91 samples were collected randomly frog farms in the countryside of the stateof Goiás of these, 50 were from frogs without clinical symptoms and 41 box of frogs with nerve sequelae characterized by lack of coordination , posture with abnormal lordosis, scoliosis and various degrees of head tilt to the left or right side. Based on these results, it is concluded that the frequency of ranavirus frogs with neurological symptoms is higher than the frequency of ranavirus frogs without apparent clinical symptoms, indicating association of clinical findings with the presence of the virus. The detection of ranavirus in animals living near the frog farms in the state of Goiás indicates that the virus is spread in the sampled detection in fish and frog farms reveals need for monitoring thespread of the virus to prevent cases of mass mortality in other species. In the second study, samples were collected randomly from 60 bullfrogs from frog farms in the state of Goiás in order to identify the agent involved in chronic, vestibular syndrome similar to clinical problems through diagnostic techniques such as transmission electron microscopy, histopathology and immunohistochemistry. Of these, 30 bullfrogs had typical clinical picture and 30 bullfrogs had become asymptomatic. At histopathology, inflammatory type of lesions with necrosis and lymphocytic infiltration were seen in the posterior regions of the cerebrum and cerebellum, with meningoencephalitis and inclusion bodies, areas of gliosis, and necrotic foci with abundant macrophages and cellular debris associated with inflammatory infiltrates. The choroid plexus showed abundant inflammatory infiltrates and lesions similar to those described above. Theselesions were also observed in the region of the semicircular canals and the central and peripheral vestibular apparatus to the front and cross cuts. These specimens subjected to staining with specific anti - iridovirus revealed the presence of positive staining in the choroid plexus region. This finding associated with the lesions described in anatomohistopatologia confirms the presence of Ranavirus. When analyzing the images obtained by electron microscopy transmission of samples from kidney and liver of bullfrog it was possible to visualize viral particles with similar characteristics to that of ranavirus, with icosahedral symmetry and size ranging from 120 - 300nm . Thus, it is concluded that the lesions of inflammatory type, described in anatomohistopatologia in the posterior central nervous system bullfrogs region, extending to the middle and inner ear, nervous symptoms associated with vestibular character indicate the presence of Ranavirus. Regarding immunohistochemistry, the presence of positive staining with specific anti- Iridovirus in the affected cells of the choroid plexus of bullfrog, associated to lesions described in anatomohistopatologia region confirms the presence of ranavirus and the presence of viral particles in the liver and kidney bullfrog, associated with inflammatory lesions in the posterior type central nervous system region, extending to the middle and inner ear, described in the present study also confirms the presence of Ranavirusin frog farms of the state of Goiás. |
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Nunes, Romão da Cunhahttp://lattes.cnpq.br/4335133347523404Rezende, Cintia Silva Minafra ehttp://lattes.cnpq.br/5841210447886226Romero, Rolando Alfredo Mazzonihttp://lattes.cnpq.br/0737991281916166Nunes, Romão da CunhaAndré, Maria Cláudia Dantas Porfirio BorgesHipólito, MárcioPorto, Regiane Nascimento GagnoMendonça, Yuri de Abreuhttp://lattes.cnpq.br/4527074042300411Mesquita, Adriano Queiroz de2015-01-30T12:35:42Z2014-05-20MESQUISTA, Adriano Queiroz de. Síndrome vestibular em Lithobates catesbeianus associada à ranavirus. 2014. 80 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2014.http://repositorio.bc.ufg.br/tede/handle/tede/4012Frog farming is an expanding activity in South America. In Brazil it can be considered consolidated, especially in the state ofGoiás. In the last few years it turned into a superintensive activity and consequently favored the emergence of diseases that pose a threat to the technical and economic viability of farms of aquatic organisms. Several diseases with high morbidity and mortality have been observed in tadpoles and frogs. Especially in the state of Goiás, two diseases affect the production cycle in the frog farms. The first manifests itself in 30 days tadpoles leading to high mortality and has been associated to the presence of Ranavirus. The second affects frogs in all stages from post-molt to the point of slaughtering. In post-metamorphic phase the animals are cachectic and 10-50 % of the populationdevelops nervous signs such as incoordination and abnormal postures chronic condition. Despite the board's neurological symptoms, some frogs are able to feed and continue to grow slowly, with few recovering normal condition. This clinical manifestation may be attributed to the anatomy of the vestibular system and hearing. Afferent bilateral vestibular signals from semicircular canal and otolith organs are essential for the stabilization of gaze, the control of posture and locomotion as well as the cognitive aspects of balance, spatial orientation and vegetative homeostasis. Considering the relevance of the topic, the objective of this study is to contribute to the elucidation of Vestibular Syndrome in Rana catesbeiana focusing in the presence of Ranavirus through the use of methods of diagnosis and histopathological techniques , real-time PCR , immunohistochemistry and electron microscopy. In the first study, we developed a method of detecting Ranavirus by real-time PCR for diagnosis of clinically healthy and diseased frogs with nerve signals in order to monitor the spread of this agent for this and other species of animals that inhabit the frog farms around the state of Goiás 91 samples were collected randomly frog farms in the countryside of the stateof Goiás of these, 50 were from frogs without clinical symptoms and 41 box of frogs with nerve sequelae characterized by lack of coordination , posture with abnormal lordosis, scoliosis and various degrees of head tilt to the left or right side. Based on these results, it is concluded that the frequency of ranavirus frogs with neurological symptoms is higher than the frequency of ranavirus frogs without apparent clinical symptoms, indicating association of clinical findings with the presence of the virus. The detection of ranavirus in animals living near the frog farms in the state of Goiás indicates that the virus is spread in the sampled detection in fish and frog farms reveals need for monitoring thespread of the virus to prevent cases of mass mortality in other species. In the second study, samples were collected randomly from 60 bullfrogs from frog farms in the state of Goiás in order to identify the agent involved in chronic, vestibular syndrome similar to clinical problems through diagnostic techniques such as transmission electron microscopy, histopathology and immunohistochemistry. Of these, 30 bullfrogs had typical clinical picture and 30 bullfrogs had become asymptomatic. At histopathology, inflammatory type of lesions with necrosis and lymphocytic infiltration were seen in the posterior regions of the cerebrum and cerebellum, with meningoencephalitis and inclusion bodies, areas of gliosis, and necrotic foci with abundant macrophages and cellular debris associated with inflammatory infiltrates. The choroid plexus showed abundant inflammatory infiltrates and lesions similar to those described above. Theselesions were also observed in the region of the semicircular canals and the central and peripheral vestibular apparatus to the front and cross cuts. These specimens subjected to staining with specific anti - iridovirus revealed the presence of positive staining in the choroid plexus region. This finding associated with the lesions described in anatomohistopatologia confirms the presence of Ranavirus. When analyzing the images obtained by electron microscopy transmission of samples from kidney and liver of bullfrog it was possible to visualize viral particles with similar characteristics to that of ranavirus, with icosahedral symmetry and size ranging from 120 - 300nm . Thus, it is concluded that the lesions of inflammatory type, described in anatomohistopatologia in the posterior central nervous system bullfrogs region, extending to the middle and inner ear, nervous symptoms associated with vestibular character indicate the presence of Ranavirus. Regarding immunohistochemistry, the presence of positive staining with specific anti- Iridovirus in the affected cells of the choroid plexus of bullfrog, associated to lesions described in anatomohistopatologia region confirms the presence of ranavirus and the presence of viral particles in the liver and kidney bullfrog, associated with inflammatory lesions in the posterior type central nervous system region, extending to the middle and inner ear, described in the present study also confirms the presence of Ranavirusin frog farms of the state of Goiás.A ranicultura é uma atividade em expansão na América do Sul podendo ser considerada consolidada no Brasil, particularmente no Estado de Goiás. Nos últimos anos, transformou-se em uma atividade superintensiva e como consequência favoreceu o aparecimento de doenças que constituem uma ameaça para a viabilidade técnica e econômica dos criatórios de organismos aquáticos. Diversas doenças com alta morbidade e mortalidade têm sido observadas emgirinos e rãs. Especialmente no estado de Goiás, duas patologias afetam o ciclo produtivo nos ranários. A primeira se manifesta em girinos de até 30 dias levando à alta taxa de mortalidade e já foi relacionada à presença de Iridovírusdo gênero Ranavirus. A segunda acomete rãs em todas as fases desde a pós-metamorfose até animais no ponto de abate. Na fase pósmetamórfica os animais apresentam-se caquéticos e de 10 a 50% da população desenvolve um quadro crônico caracterizado clinicamente por sinais nervosos como falta de coordenação e posturas anormais. Apesar do quadro com sintomatologia nervosa, algumas rãs conseguem se alimentar e continuam crescendo lentamente, sendo que poucas recuperam a condição normal. Essamanifestação clínica pode ser atribuída à própria anatomia do sistema vestibular e do aparelho auditivo. Sinais aferentes vestibulares bilaterais de canal semicircular e órgãos otolíticos são essenciais para a estabilização do olhar, o controle da postura e locomoção, bem como para os aspectos cognitivos de equilíbrio, orientação espacial e homeostase vegetativa. Considerando a relevância do tema, objetivou-se como presente estudo contribuir para elucidação da Síndrome Vestibular em Rana catesbeiana com foco na presença de Ranavirus por meio do emprego de métodos de diagnóstico como técnicas histopatológicas, PCR em tempo real, microscopia eletrônica e imunohistoquímica. No primeiro estudo, foi desenvolvido um método de detecção de Ranavirus por PCR em tempo real para diagnóstico em rãs clinicamente sadias e em rãs enfermas com sinais nervosos, com o objetivo de monitorar a disseminação desse agente para essa e outras espécies de animais que habitam o entorno dos ranários do estado de Goiás. Foram colhidas 91 amostras distribuídas aleatoriamente em ranários da zona rural do estado de Goiás. Dessas, 50 eram provenientes de rãs sem sintomatologia clínica e 41 de rãs com quadro de sequelas nervosas caracterizado por falta de coordenação, postura anormal com lordose, diversos graus de escoliose e desvio da cabeça para os lados esquerdo ou direito. Diante dos resultados obtidos, conclui-se que a frequência de Ranavirusem rãs com sintomatologia nervosa é superior à frequência de Ranavirus em rãs sem sintomatologia clínica aparente, indicando associação do quadro clínico com a presença do vírus. A detecção de Ranavirusem animais que habitam o entorno dos ranários do estado de Goiás indica que o vírus encontra-se disseminado nos ranários amostrados e a detecção em peixes revela necessidade de monitoramento da disseminação do vírus para evitar casos de mortalidade em massa em outras espécies. No segundo estudo, foram colhidas aleatoriamente amostras de 60 rãs da espécie Rana catesbeiana provenientes de ranários do estado de Goiás com o objetivo de identificar o agente envolvido nos quadros clínicos crônicos, similares à síndrome vestibular, por meio de técnicas de diagnóstico como microscopia eletrônica de transmissão, anatomohistopatologia e imunohistoquímica. Dessas, 30 apresentavam quadro clínico característico e 30 apresentavam-se assintomáticas. À anatomohistopatologia foram identificadas lesões do tipo inflamatória com necrose e infiltrado linfocitário nas regiões posteriores do encéfalo e cerebelo, com meningoencefalite e corpúsculos de inclusão, regiões de gliose, e focos necróticos com abundantes macrófagos e restos celulares associados a infiltrados inflamatórios. O plexo coroide apresentou infiltrado inflamatório abundante e lesões semelhantes às descritas anteriormente. Essas lesões também foram observadas na região dos canais semicirculares e no aparelho vestibular central e periférico aos cortes frontal e transversal. A leitura das lâminas submetidas à coloração com anticorpo específico anti-iridovírus revelou a presença de marcação positiva na região do plexo coroide. Essa constatação associada às lesões descritas na anatomohistopatologia confirma a presença de Ranavirus. Ao analisar as imagens obtidas por microscopia eletrônica de transmissão de amostras de rim e fígado foi possível visualizar partículas virais com características semelhantes à dos Ranavirus, ou seja, com simetria icosaédrica e tamanho variando de 120-300nm. Desse modo, conclui-se que as lesões do tipo inflamatórias, descritas na anatomohistopatologia, na região do sistema nervoso central posterior, com extensão para o ouvido interno e médio, associadas à sintomatologia nervosa de caráter vestibular indicam a presença de Ranavirus. Em relação à imunohistoquímica, a presença de marcação positiva com anticorpo específico antiiridovirus em células afetadas da região do plexo coroide, associada às lesões descritas na anatomohistopatologia confirma a presença de Ranavirus e a presença de partículas virais em fígado e rim, associada às lesões de tipo inflamatórias na região do sistema nervoso central posterior, com extensão para o ouvido interno e médio, descritas no presente estudo, também confirma a presença de Ranavirus em rãs de criação de ranários do estado de GoiásSubmitted by Cássia Santos (cassia.bcufg@gmail.com) on 2015-01-30T10:37:22Z No. of bitstreams: 2 license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Tese - Adriano Queiroz de Mesquita - 2014.pdf: 6614236 bytes, checksum: 0e7a5056a14cf2c542e7bae25c3f574b (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2015-01-30T12:35:42Z (GMT) No. of bitstreams: 2 license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Tese - Adriano Queiroz de Mesquita - 2014.pdf: 6614236 bytes, checksum: 0e7a5056a14cf2c542e7bae25c3f574b (MD5)Made available in DSpace on 2015-01-30T12:35:42Z (GMT). No. of bitstreams: 2 license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Tese - Adriano Queiroz de Mesquita - 2014.pdf: 6614236 bytes, checksum: 0e7a5056a14cf2c542e7bae25c3f574b (MD5) Previous issue date: 2014-05-20Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfhttp://repositorio.bc.ufg.br/tede/retrieve/15978/Tese%20-%20Adriano%20Queiroz%20de%20Mesquita%20-%202014.pdf.jpgporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciência Animal (EVZ)UFGBrasilEscola de Veterinária e Zootecnia - EVZ (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessDiagnósticoImunohistoquímicaRaniculturaMicroscopia eletrônicaDiagnosisImmunohistochemistryRanicultureElectron microscopyMEDICINA VETERINARIA PREVENTIVA::SAUDE ANIMAL (PROGRAMAS SANITARIOS)Síndrome vestibular em Lithobates catesbeianus associada à ranavirusVestibular syndrome in Lithobates catesbeianus associated to ranavirusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis4581960685150189167600600600600-621755211424909458267029613561320070562075167498588264571reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGORIGINALTese - Adriano Queiroz de Mesquita - 2014.pdfTese - Adriano Queiroz de Mesquita - 2014.pdfTese - Adriano Queiroz de Mesquita - 2014application/pdf6614236http://repositorio.bc.ufg.br/tede/bitstreams/75c1f554-1ce9-40d9-8dd5-08812944a31c/download0e7a5056a14cf2c542e7bae25c3f574bMD52LICENSElicense.txtlicense.txttext/plain; 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dc.title.eng.fl_str_mv |
Síndrome vestibular em Lithobates catesbeianus associada à ranavirus |
dc.title.alternative.eng.fl_str_mv |
Vestibular syndrome in Lithobates catesbeianus associated to ranavirus |
title |
Síndrome vestibular em Lithobates catesbeianus associada à ranavirus |
spellingShingle |
Síndrome vestibular em Lithobates catesbeianus associada à ranavirus Mesquita, Adriano Queiroz de Diagnóstico Imunohistoquímica Ranicultura Microscopia eletrônica Diagnosis Immunohistochemistry Raniculture Electron microscopy MEDICINA VETERINARIA PREVENTIVA::SAUDE ANIMAL (PROGRAMAS SANITARIOS) |
title_short |
Síndrome vestibular em Lithobates catesbeianus associada à ranavirus |
title_full |
Síndrome vestibular em Lithobates catesbeianus associada à ranavirus |
title_fullStr |
Síndrome vestibular em Lithobates catesbeianus associada à ranavirus |
title_full_unstemmed |
Síndrome vestibular em Lithobates catesbeianus associada à ranavirus |
title_sort |
Síndrome vestibular em Lithobates catesbeianus associada à ranavirus |
author |
Mesquita, Adriano Queiroz de |
author_facet |
Mesquita, Adriano Queiroz de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Nunes, Romão da Cunha |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4335133347523404 |
dc.contributor.advisor-co1.fl_str_mv |
Rezende, Cintia Silva Minafra e |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/5841210447886226 |
dc.contributor.advisor-co2.fl_str_mv |
Romero, Rolando Alfredo Mazzoni |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://lattes.cnpq.br/0737991281916166 |
dc.contributor.referee1.fl_str_mv |
Nunes, Romão da Cunha |
dc.contributor.referee2.fl_str_mv |
André, Maria Cláudia Dantas Porfirio Borges |
dc.contributor.referee3.fl_str_mv |
Hipólito, Márcio |
dc.contributor.referee4.fl_str_mv |
Porto, Regiane Nascimento Gagno |
dc.contributor.referee5.fl_str_mv |
Mendonça, Yuri de Abreu |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4527074042300411 |
dc.contributor.author.fl_str_mv |
Mesquita, Adriano Queiroz de |
contributor_str_mv |
Nunes, Romão da Cunha Rezende, Cintia Silva Minafra e Romero, Rolando Alfredo Mazzoni Nunes, Romão da Cunha André, Maria Cláudia Dantas Porfirio Borges Hipólito, Márcio Porto, Regiane Nascimento Gagno Mendonça, Yuri de Abreu |
dc.subject.por.fl_str_mv |
Diagnóstico Imunohistoquímica Ranicultura Microscopia eletrônica |
topic |
Diagnóstico Imunohistoquímica Ranicultura Microscopia eletrônica Diagnosis Immunohistochemistry Raniculture Electron microscopy MEDICINA VETERINARIA PREVENTIVA::SAUDE ANIMAL (PROGRAMAS SANITARIOS) |
dc.subject.eng.fl_str_mv |
Diagnosis Immunohistochemistry Raniculture Electron microscopy |
dc.subject.cnpq.fl_str_mv |
MEDICINA VETERINARIA PREVENTIVA::SAUDE ANIMAL (PROGRAMAS SANITARIOS) |
description |
Frog farming is an expanding activity in South America. In Brazil it can be considered consolidated, especially in the state ofGoiás. In the last few years it turned into a superintensive activity and consequently favored the emergence of diseases that pose a threat to the technical and economic viability of farms of aquatic organisms. Several diseases with high morbidity and mortality have been observed in tadpoles and frogs. Especially in the state of Goiás, two diseases affect the production cycle in the frog farms. The first manifests itself in 30 days tadpoles leading to high mortality and has been associated to the presence of Ranavirus. The second affects frogs in all stages from post-molt to the point of slaughtering. In post-metamorphic phase the animals are cachectic and 10-50 % of the populationdevelops nervous signs such as incoordination and abnormal postures chronic condition. Despite the board's neurological symptoms, some frogs are able to feed and continue to grow slowly, with few recovering normal condition. This clinical manifestation may be attributed to the anatomy of the vestibular system and hearing. Afferent bilateral vestibular signals from semicircular canal and otolith organs are essential for the stabilization of gaze, the control of posture and locomotion as well as the cognitive aspects of balance, spatial orientation and vegetative homeostasis. Considering the relevance of the topic, the objective of this study is to contribute to the elucidation of Vestibular Syndrome in Rana catesbeiana focusing in the presence of Ranavirus through the use of methods of diagnosis and histopathological techniques , real-time PCR , immunohistochemistry and electron microscopy. In the first study, we developed a method of detecting Ranavirus by real-time PCR for diagnosis of clinically healthy and diseased frogs with nerve signals in order to monitor the spread of this agent for this and other species of animals that inhabit the frog farms around the state of Goiás 91 samples were collected randomly frog farms in the countryside of the stateof Goiás of these, 50 were from frogs without clinical symptoms and 41 box of frogs with nerve sequelae characterized by lack of coordination , posture with abnormal lordosis, scoliosis and various degrees of head tilt to the left or right side. Based on these results, it is concluded that the frequency of ranavirus frogs with neurological symptoms is higher than the frequency of ranavirus frogs without apparent clinical symptoms, indicating association of clinical findings with the presence of the virus. The detection of ranavirus in animals living near the frog farms in the state of Goiás indicates that the virus is spread in the sampled detection in fish and frog farms reveals need for monitoring thespread of the virus to prevent cases of mass mortality in other species. In the second study, samples were collected randomly from 60 bullfrogs from frog farms in the state of Goiás in order to identify the agent involved in chronic, vestibular syndrome similar to clinical problems through diagnostic techniques such as transmission electron microscopy, histopathology and immunohistochemistry. Of these, 30 bullfrogs had typical clinical picture and 30 bullfrogs had become asymptomatic. At histopathology, inflammatory type of lesions with necrosis and lymphocytic infiltration were seen in the posterior regions of the cerebrum and cerebellum, with meningoencephalitis and inclusion bodies, areas of gliosis, and necrotic foci with abundant macrophages and cellular debris associated with inflammatory infiltrates. The choroid plexus showed abundant inflammatory infiltrates and lesions similar to those described above. Theselesions were also observed in the region of the semicircular canals and the central and peripheral vestibular apparatus to the front and cross cuts. These specimens subjected to staining with specific anti - iridovirus revealed the presence of positive staining in the choroid plexus region. This finding associated with the lesions described in anatomohistopatologia confirms the presence of Ranavirus. When analyzing the images obtained by electron microscopy transmission of samples from kidney and liver of bullfrog it was possible to visualize viral particles with similar characteristics to that of ranavirus, with icosahedral symmetry and size ranging from 120 - 300nm . Thus, it is concluded that the lesions of inflammatory type, described in anatomohistopatologia in the posterior central nervous system bullfrogs region, extending to the middle and inner ear, nervous symptoms associated with vestibular character indicate the presence of Ranavirus. Regarding immunohistochemistry, the presence of positive staining with specific anti- Iridovirus in the affected cells of the choroid plexus of bullfrog, associated to lesions described in anatomohistopatologia region confirms the presence of ranavirus and the presence of viral particles in the liver and kidney bullfrog, associated with inflammatory lesions in the posterior type central nervous system region, extending to the middle and inner ear, described in the present study also confirms the presence of Ranavirusin frog farms of the state of Goiás. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-05-20 |
dc.date.accessioned.fl_str_mv |
2015-01-30T12:35:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MESQUISTA, Adriano Queiroz de. Síndrome vestibular em Lithobates catesbeianus associada à ranavirus. 2014. 80 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/4012 |
identifier_str_mv |
MESQUISTA, Adriano Queiroz de. Síndrome vestibular em Lithobates catesbeianus associada à ranavirus. 2014. 80 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2014. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/4012 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
4581960685150189167 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
-6217552114249094582 |
dc.relation.cnpq.fl_str_mv |
6702961356132007056 |
dc.relation.sponsorship.fl_str_mv |
2075167498588264571 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Ciência Animal (EVZ) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Escola de Veterinária e Zootecnia - EVZ (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
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Universidade Federal de Goiás (UFG) |
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UFG |
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UFG |
reponame_str |
Repositório Institucional da UFG |
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Repositório Institucional da UFG |
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