Ação citotóxica e antioxidante da beta lapachona em células endoteliais

• Ano de defesa: 2017
• Autor(a) principal: Gonçalves, Patricia de Almeida Machado
• Orientador(a): Araújo, Eugênio Gonçalves de
• Banca de defesa: Araújo, Eugênio Gonçalves de, Santin, Ana Paula Iglesias, Porto, Regiani Nascimento Gagno
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Ciência Animal (EVZ)
• Departamento: Escola de Veterinária e Zootecnia - EVZ (RG)
• País: Brasil
• Palavras-chave em Português: Isquemia e reperfusão; Naftoquinona; Proteção celular; Radicais livres
• Palavras-chave em Inglês: Cell protection; Free radicals; Ischemia/reperfusion; Naphtoquinone
• Área do conhecimento CNPq: MEDICINA VETERINARIA::PATOLOGIA ANIMAL
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/8138

Oxidative stress is the overproduction of reactive oxygen species that cause cell damage and even the death. It is a mechanism related to ischemia and reperfusion, a process that affects several organs and is involved in neurodegenerative diseases, cardiac and/or renal insufficiencies, hepatic dysfunction, among others. The discovery of new antioxidant substances derived from natural sources, is important for the prevention and treatment of these diseases. Beta lapachone is a substance with antioxidant action obtained from sawdust of Ipe wood, a symbolic tree from the Brazilian cerrado. This study aimed to analyze the cytotoxic and antioxidant action of beta lapachone in endothelial cells, in vitro. EA.hy 926 lineage cells were subcultured, treated with different concentrations of beta lapachone and subjected to ischemia and reperfusion. Cytotoxicity and antioxidant action were assessed through the cellular viability analysis by the tetrazolium reduction method. The averages were verified by the Tukey test (5% of significance) and by variance analysis. There was a dose-dependent progression of cytotoxicity in the non-ischemia/reperfusion (control) group and cell viability increase in the ischemia/reperfusion group. However, there was no statistical difference between the concentrations and between groups. Considering the settings of this experiment, Beta lapachone did not present cytotoxic or antioxidant action on endothelial cells.