Preparação e caracterização de fluidos magnéticos de maghemita funcionalizada com o aminoácido L-Lisina e dextrana para carreamento de plasmídeos

Detalhes bibliográficos
Ano de defesa: 2016
Autor(a) principal: Silva, Breiner Gabriel Canedo lattes
Orientador(a): Sartoratto, Patrícia Pommé Confessori lattes
Banca de defesa: Sartoratto, Patrícia Pommé Confessori, Lacava, Zulmira Guerrero Marques, Carneiro, Bruno Moreira, Chagas, Rafael Pavão das
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Goiás
Programa de Pós-Graduação: Programa de Pós-graduação em Química (IQ)
Departamento: Instituto de Química - IQ (RG)
País: Brasil
Palavras-chave em Português:
Nanopartículas magnéticas
Maghemita
Dextrana
Plasmídeos
Palavras-chave em Inglês:
Magnetic nanoparticles
Maghemite
Plasmids
Área do conhecimento CNPq:
CIENCIAS EXATAS E DA TERRA::QUIMICA
Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/5810
Resumo: In this work magnetic nanoparticles composed of maghemite coated with L-lysine or DL-Histidine (crystallite average diameter between 4.5 to 8.1 nm.) were synthesized via the Fe (II) and Fe (III) coprecipitation method. The synthesized solids were dispersed in water to obtain aqueous suspensions called magnetic fluids. The samples were characterized by X-ray diffraction, infrared spectroscopy, thermogravimetry, UV-Vis spectrophotometry, transmission electron microscopy, electrophoretic and dynamic light scattering. The suspended colloidal nanoparticles showed positive zeta potential at physiological pH, which enables the adsorption of negatively charged molecules of chromosomal and plasmid DNA on their surfaces. Their hydrodynamic radius were smaller than those reported in the literature (100nm) allowing phagocytosis of the nanostructure. A maghemite/L-Lysine sample was selected for DNA adsorption (chromosomal DNA of Humicola grisea fungus and shRNA and GFP encoding plasmids) and subsequent coating with dextran, for protection of its genetic information. Plasmids and dextran adsorption was evidenced by the increasing of the hydrodynamic radius of the samples, and the presence of DNA and dextran related bands in the infrared.
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spelling Sartoratto, Patrícia Pommé Confessorihttp://lattes.cnpq.br/9777959525393785Lacava, Zulmira Guerrero Marqueshttp://lattes.cnpq.br/8139627239707745Sartoratto, Patrícia Pommé ConfessoriLacava, Zulmira Guerrero MarquesCarneiro, Bruno MoreiraChagas, Rafael Pavão dashttp://lattes.cnpq.br/2204297783924908Silva, Breiner Gabriel Canedo2016-08-03T15:18:38Z2016-07-01CANEDO SILVA, B. G. Preparação e caracterização de fluidos magnéticos de maghemita funcionalizada com o aminoácido L-Lisina e dextrana para carreamento de plasmídeos. 2016. 87 f. Dissertação (Mestrado em Química) - Universidade Federal de Goiás, Goiânia, 2016.http://repositorio.bc.ufg.br/tede/handle/tede/5810In this work magnetic nanoparticles composed of maghemite coated with L-lysine or DL-Histidine (crystallite average diameter between 4.5 to 8.1 nm.) were synthesized via the Fe (II) and Fe (III) coprecipitation method. The synthesized solids were dispersed in water to obtain aqueous suspensions called magnetic fluids. The samples were characterized by X-ray diffraction, infrared spectroscopy, thermogravimetry, UV-Vis spectrophotometry, transmission electron microscopy, electrophoretic and dynamic light scattering. The suspended colloidal nanoparticles showed positive zeta potential at physiological pH, which enables the adsorption of negatively charged molecules of chromosomal and plasmid DNA on their surfaces. Their hydrodynamic radius were smaller than those reported in the literature (100nm) allowing phagocytosis of the nanostructure. A maghemite/L-Lysine sample was selected for DNA adsorption (chromosomal DNA of Humicola grisea fungus and shRNA and GFP encoding plasmids) and subsequent coating with dextran, for protection of its genetic information. Plasmids and dextran adsorption was evidenced by the increasing of the hydrodynamic radius of the samples, and the presence of DNA and dextran related bands in the infrared.Neste trabalho, foram sintetizadas nanopartículas magnéticas constituídas de maghemita funcionalizada com L-Lisina ou DL-Histidina (cristalitos com diâmetro médio de 4,5 a 8,1 nm), pelo método da coprecipitação dos íons Fe (II) e (III). Os sólidos obtidos foram dispersos em água para obtenção de suspensões aquosas denominadas fluidos magnéticos. As amostras obtidas foram caracterizadas pelas técnicas de difração de raios X, espectroscopia de infravermelho, termogravimetria, espectrofotometria UV-Vis, microscopia eletrônica de transmissão e espalhamento de luz dinâmico e eletroforético. As nanopartículas em suspensão apresentaram potencial zeta positivo em pH fisiológico, possibilitando assim adsorção de moléculas de DNA (cromossômico e plasmidial) negativamente carregadas, além de um raio hidrodinâmico inferior ao relatado na literatura (100 nm) para que ocorra a fagocitose das nanoestruturas. Uma amostra maghemita/L-Lisina foi selecionada para adsorção de DNA (cromossômico de fungo Humícola grísea e de plasmídeos codificadores de shRNA e GFP) e posterior recobrimento com dextrana, para proteção de sua informação genética. A adsorção dos plasmídeos e da dextrana foi evidenciada pelo aumento do raio hidrodinâmico das amostras e pela presença das bandas relativas à dextrana e ao DNA nos espectros de infravermelho.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Química (IQ)UFGBrasilInstituto de Química - IQ (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessNanopartículas magnéticasMaghemitaDextranaPlasmídeosMagnetic nanoparticlesMaghemitePlasmidsCIENCIAS EXATAS E DA TERRA::QUIMICAPreparação e caracterização de fluidos magnéticos de maghemita funcionalizada com o aminoácido L-Lisina e dextrana para carreamento de plasmídeosPreparation and characterization of magnetic fluids based on maghemite functionalized with L-lysine amino acid and dextran for plasmids deliveryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis663693921325415158600600600600782606674374119727815717003253031171952075167498588264571reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv Preparação e caracterização de fluidos magnéticos de maghemita funcionalizada com o aminoácido L-Lisina e dextrana para carreamento de plasmídeos
dc.title.alternative.eng.fl_str_mv Preparation and characterization of magnetic fluids based on maghemite functionalized with L-lysine amino acid and dextran for plasmids delivery
title Preparação e caracterização de fluidos magnéticos de maghemita funcionalizada com o aminoácido L-Lisina e dextrana para carreamento de plasmídeos
spellingShingle Preparação e caracterização de fluidos magnéticos de maghemita funcionalizada com o aminoácido L-Lisina e dextrana para carreamento de plasmídeos
Silva, Breiner Gabriel Canedo
Nanopartículas magnéticas
Maghemita
Dextrana
Plasmídeos
Magnetic nanoparticles
Maghemite
Plasmids
CIENCIAS EXATAS E DA TERRA::QUIMICA
title_short Preparação e caracterização de fluidos magnéticos de maghemita funcionalizada com o aminoácido L-Lisina e dextrana para carreamento de plasmídeos
title_full Preparação e caracterização de fluidos magnéticos de maghemita funcionalizada com o aminoácido L-Lisina e dextrana para carreamento de plasmídeos
title_fullStr Preparação e caracterização de fluidos magnéticos de maghemita funcionalizada com o aminoácido L-Lisina e dextrana para carreamento de plasmídeos
title_full_unstemmed Preparação e caracterização de fluidos magnéticos de maghemita funcionalizada com o aminoácido L-Lisina e dextrana para carreamento de plasmídeos
title_sort Preparação e caracterização de fluidos magnéticos de maghemita funcionalizada com o aminoácido L-Lisina e dextrana para carreamento de plasmídeos
author Silva, Breiner Gabriel Canedo
author_facet Silva, Breiner Gabriel Canedo
author_role author
dc.contributor.advisor1.fl_str_mv Sartoratto, Patrícia Pommé Confessori
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/9777959525393785
dc.contributor.advisor-co1.fl_str_mv Lacava, Zulmira Guerrero Marques
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/8139627239707745
dc.contributor.referee1.fl_str_mv Sartoratto, Patrícia Pommé Confessori
dc.contributor.referee2.fl_str_mv Lacava, Zulmira Guerrero Marques
dc.contributor.referee3.fl_str_mv Carneiro, Bruno Moreira
dc.contributor.referee4.fl_str_mv Chagas, Rafael Pavão das
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/2204297783924908
dc.contributor.author.fl_str_mv Silva, Breiner Gabriel Canedo
contributor_str_mv Sartoratto, Patrícia Pommé Confessori
Lacava, Zulmira Guerrero Marques
Sartoratto, Patrícia Pommé Confessori
Lacava, Zulmira Guerrero Marques
Carneiro, Bruno Moreira
Chagas, Rafael Pavão das
dc.subject.por.fl_str_mv Nanopartículas magnéticas
Maghemita
Dextrana
Plasmídeos
topic Nanopartículas magnéticas
Maghemita
Dextrana
Plasmídeos
Magnetic nanoparticles
Maghemite
Plasmids
CIENCIAS EXATAS E DA TERRA::QUIMICA
dc.subject.eng.fl_str_mv Magnetic nanoparticles
Maghemite
Plasmids
dc.subject.cnpq.fl_str_mv CIENCIAS EXATAS E DA TERRA::QUIMICA
description In this work magnetic nanoparticles composed of maghemite coated with L-lysine or DL-Histidine (crystallite average diameter between 4.5 to 8.1 nm.) were synthesized via the Fe (II) and Fe (III) coprecipitation method. The synthesized solids were dispersed in water to obtain aqueous suspensions called magnetic fluids. The samples were characterized by X-ray diffraction, infrared spectroscopy, thermogravimetry, UV-Vis spectrophotometry, transmission electron microscopy, electrophoretic and dynamic light scattering. The suspended colloidal nanoparticles showed positive zeta potential at physiological pH, which enables the adsorption of negatively charged molecules of chromosomal and plasmid DNA on their surfaces. Their hydrodynamic radius were smaller than those reported in the literature (100nm) allowing phagocytosis of the nanostructure. A maghemite/L-Lysine sample was selected for DNA adsorption (chromosomal DNA of Humicola grisea fungus and shRNA and GFP encoding plasmids) and subsequent coating with dextran, for protection of its genetic information. Plasmids and dextran adsorption was evidenced by the increasing of the hydrodynamic radius of the samples, and the presence of DNA and dextran related bands in the infrared.
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-08-03T15:18:38Z
dc.date.issued.fl_str_mv 2016-07-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv CANEDO SILVA, B. G. Preparação e caracterização de fluidos magnéticos de maghemita funcionalizada com o aminoácido L-Lisina e dextrana para carreamento de plasmídeos. 2016. 87 f. Dissertação (Mestrado em Química) - Universidade Federal de Goiás, Goiânia, 2016.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/5810
identifier_str_mv CANEDO SILVA, B. G. Preparação e caracterização de fluidos magnéticos de maghemita funcionalizada com o aminoácido L-Lisina e dextrana para carreamento de plasmídeos. 2016. 87 f. Dissertação (Mestrado em Química) - Universidade Federal de Goiás, Goiânia, 2016.
url http://repositorio.bc.ufg.br/tede/handle/tede/5810
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dc.publisher.none.fl_str_mv Universidade Federal de Goiás
dc.publisher.program.fl_str_mv Programa de Pós-graduação em Química (IQ)
dc.publisher.initials.fl_str_mv UFG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Instituto de Química - IQ (RG)
publisher.none.fl_str_mv Universidade Federal de Goiás
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