Aspectos moleculares da interação patogênica entre Sclerotinia sclerotiorum e Phaseolus vulgaris L.
Ano de defesa: | 2016 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Goiás
|
Programa de Pós-Graduação: |
Programa de Pós-graduação em Ciências Biológicas (ICB)
|
Departamento: |
Instituto de Ciências Biológicas - ICB (RG)
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.bc.ufg.br/tede/handle/tede/9170 |
Resumo: | White mold caused by Sclerotinia sclerotiorum (Lib.) De Bary is one of the most devastating diseases of common bean (Phaseolus vulgaris L.) and others crops. This study aimed to achieving a better understanding of the pathosystem S. sclerotiorum-P. vulgaris identifying genes potentially involved in this interaction. Two different approaches were used to evaluate this pathosystem: 1) susceptible cultivar Pérola (BRS) of P. vulgaris; and 2) treatment of this cultivar with the resistance inducer methyl jasmonate (MeJA). For both situations, a suppression subtractive hybridization (SSH) cDNA library approach was used to identify differentially expressed genes in the pathogen and the host plant. In the interaction between the susceptible cultivar and S. sclerotiorum 979 unigenes (430 contigs and 549 singletons) were inentified with 29% of these unigenes were categorized as fungal genes, related to cell wall degradation, proteins degradation, oxidative stress among other. And 58,3% were categorized as plant genes related to energetic metabolism, protein degradation, response to biotic and abiotic stress. The selection of differential clones of their libraries SSH has been validated by reverse transcription and quantitative real-time PCR (RT-qPCR). Were validated genes related to pathogenicity and virulence factors of S. sclerotiorum, such as genes encoding enzymes that degrade plant cell walls (polygalacturonases, cellulases, hemicellulases and proteases). And besides these, defense-related genes of the host plant as: pathogenesis-relateds genes (PR1, PR2, and PR3), phenylpropanoid pathway (Isof, FPS1, and 4CL), and involved in defense and stress-related categories (Lox, Hiprp, GST, POD, and DOX). Exogenous MeJA application induced partial resistance agains S. sclerotiorum in plants as well as a consistent increase in pathogenesis- related protein activities. Furthermore, the validation of SSH made by RT-qPCR showed differential expression of important unigenes over time, such as: pathogenesis related protein PR3 (chitinase class I), PvCallose (callose synthase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like), and a polygalacturonase inhibitor protein (PGIP). The data obtained in this study have highlighted some of the mechanisms involved in the pathogenicity of the fungus, as well as defense responses produced by the plant in two conditions evaluated, thus contributing to a better understanding of pathosystem. |
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Silva, Silvana Petrofeza dahttp://lattes.cnpq.br/6823998544968373Cunha, Marcos GomesVianello, Rosana PereiraMelo, Hyrandir Cabral deLobo Júnior, Murillohttp://lattes.cnpq.br/7759891178725653Oliveira, Marília Barros2018-12-21T10:11:06Z2016-02-26OLIVEIRA, M. B. Aspectos moleculares da interação patogênica entre Sclerotinia sclerotiorum e Phaseolus vulgaris L. 2016. 115 f. Tese (Doutorado em Biologia) - Universidade Federal de Goiás, Goiânia, 2016.http://repositorio.bc.ufg.br/tede/handle/tede/9170White mold caused by Sclerotinia sclerotiorum (Lib.) De Bary is one of the most devastating diseases of common bean (Phaseolus vulgaris L.) and others crops. This study aimed to achieving a better understanding of the pathosystem S. sclerotiorum-P. vulgaris identifying genes potentially involved in this interaction. Two different approaches were used to evaluate this pathosystem: 1) susceptible cultivar Pérola (BRS) of P. vulgaris; and 2) treatment of this cultivar with the resistance inducer methyl jasmonate (MeJA). For both situations, a suppression subtractive hybridization (SSH) cDNA library approach was used to identify differentially expressed genes in the pathogen and the host plant. In the interaction between the susceptible cultivar and S. sclerotiorum 979 unigenes (430 contigs and 549 singletons) were inentified with 29% of these unigenes were categorized as fungal genes, related to cell wall degradation, proteins degradation, oxidative stress among other. And 58,3% were categorized as plant genes related to energetic metabolism, protein degradation, response to biotic and abiotic stress. The selection of differential clones of their libraries SSH has been validated by reverse transcription and quantitative real-time PCR (RT-qPCR). Were validated genes related to pathogenicity and virulence factors of S. sclerotiorum, such as genes encoding enzymes that degrade plant cell walls (polygalacturonases, cellulases, hemicellulases and proteases). And besides these, defense-related genes of the host plant as: pathogenesis-relateds genes (PR1, PR2, and PR3), phenylpropanoid pathway (Isof, FPS1, and 4CL), and involved in defense and stress-related categories (Lox, Hiprp, GST, POD, and DOX). Exogenous MeJA application induced partial resistance agains S. sclerotiorum in plants as well as a consistent increase in pathogenesis- related protein activities. Furthermore, the validation of SSH made by RT-qPCR showed differential expression of important unigenes over time, such as: pathogenesis related protein PR3 (chitinase class I), PvCallose (callose synthase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like), and a polygalacturonase inhibitor protein (PGIP). The data obtained in this study have highlighted some of the mechanisms involved in the pathogenicity of the fungus, as well as defense responses produced by the plant in two conditions evaluated, thus contributing to a better understanding of pathosystem.O mofo branco causado pelo fungo Sclerotinia sclerotiorum (Lib.) de Bary é uma das doenças mais devastadoras do feijoeiro comum (Phaseolus vulgaris L.) e de outras culturas. Este trabalho teve como objetivo obter uma melhor compreeensão do patossistema S. sclerotiorum-P. vulgaris identificando os genes potencialmente envolvidos durante essa interação. Duas diferentes abordagens foram utilizadas para avaliar esse patossistema: 1) cultivar suscetível Pérola (BRS) de P. vulgaris; e 2) tratamento dessa cultivar com o indutor de resistência metil jasmonato (MeJA). Para as duas situações, a metodologia de biblioteca de cDNA construída a partir da hibridização subtrativa por supressão (HSS) foi usada para identificar genes diferencialmente expressos no patógeno e na planta hospedeira. Na interaçao entre a cultivar suscetível e S. sclerotiorum 979 unigenes (430 contigs e 549 singletons) foram identificados, sendo que 29% desses unigenes foram categorizados como genes do fungo, relacionados à degradação de parede celular, degradação de proteínas, estresse oxidativo dentre outros. E 58,3 % foram categorizados como genes de plantas relacionados a metabolismo energético, degradação de proteínas e resposta a estresse biótico e abiótico. A seleção dos clones diferenciais das respectivas bibliotecas HSS foi validada por transcrição reversa e PCR quantitativa em Tempo Real (RT-qPCR). Foram validados genes relacionados à patogenicidade e fatores de virulência de S. sclerotiorum, como: genes que codificam para enzimas que degradam parede celular da planta (poligalacturonases, celulases, hemicelulases and proteases). E além desses, genes relacionados à defesa da planta hospedeira como: genes relacionados à patogenicidade (PR1, PR2, e PR3), via dos fenilpropanoides (Isof, FPS1 e 4CL), e genes envolvidos em defesa ou relacionados à estresse (Lox, Hiprp, GST, POD e DOX). A aplicação exógena de MeJA induziu resistência parcial contra S. sclerotiorum em plantas e determinou um consistente aumento na atividade de proteínas relacionadas à patogênese. Além disso, a validação da HSS feita por RT-qPCR demonstrou a expressão diferencial de importantes unigenes ao longo do tempo, tais como: proteínas relacionadas à patogênese PR3 (quitinase classe 1), PvCallose (Calose sintase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like) e proteínas inibidoras de poligalacturonases (Pvpgip1-4). Os dados obtidos neste estudo permitiram evidenciar alguns dos mecanismos envolvidos na patogenicidade do fungo, bem como as respostas de defesa produzidas pela planta nas duas condições avaliadas, contribuindo assim para uma melhor compreensão do patossistema.Submitted by Ana Caroline Costa (ana_caroline212@hotmail.com) on 2018-12-17T16:37:33Z No. of bitstreams: 2 Tese - Marília Barros Oliveira - 2016.pdf: 4229302 bytes, checksum: dae90d506cc1da8e2cf2c81e4db4eaed (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2018-12-21T10:11:06Z (GMT) No. of bitstreams: 2 Tese - Marília Barros Oliveira - 2016.pdf: 4229302 bytes, checksum: dae90d506cc1da8e2cf2c81e4db4eaed (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2018-12-21T10:11:06Z (GMT). No. of bitstreams: 2 Tese - Marília Barros Oliveira - 2016.pdf: 4229302 bytes, checksum: dae90d506cc1da8e2cf2c81e4db4eaed (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016-02-26Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciências Biológicas (ICB)UFGBrasilInstituto de Ciências Biológicas - ICB (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessExpressão gênicaFeijão comumHibridização subtrativa por supressãoMofo brancoTranscritos induzidos por estresseCommon beanGene expressionSuppression subtractive hybridizationStress-induced transcriptsWhite moldCIENCIAS BIOLOGICAS::BIOQUIMICAAspectos moleculares da interação patogênica entre Sclerotinia sclerotiorum e Phaseolus vulgaris L.Molecular aspects of the pathogenic interaction between Sclerotinia sclerotiorum and Phaseolus vulgaris L.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-3362730732320261554600600600600-38727721178273734048934779903292661142075167498588264571reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGORIGINALTese - Marília Barros Oliveira - 2016.pdfTese - Marília Barros Oliveira - 2016.pdfapplication/pdf4229302http://repositorio.bc.ufg.br/tede/bitstreams/2ddaad95-ccfc-4248-9879-dfcd85fa0799/downloaddae90d506cc1da8e2cf2c81e4db4eaedMD55LICENSElicense.txtlicense.txttext/plain; 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dc.title.eng.fl_str_mv |
Aspectos moleculares da interação patogênica entre Sclerotinia sclerotiorum e Phaseolus vulgaris L. |
dc.title.alternative.eng.fl_str_mv |
Molecular aspects of the pathogenic interaction between Sclerotinia sclerotiorum and Phaseolus vulgaris L. |
title |
Aspectos moleculares da interação patogênica entre Sclerotinia sclerotiorum e Phaseolus vulgaris L. |
spellingShingle |
Aspectos moleculares da interação patogênica entre Sclerotinia sclerotiorum e Phaseolus vulgaris L. Oliveira, Marília Barros Expressão gênica Feijão comum Hibridização subtrativa por supressão Mofo branco Transcritos induzidos por estresse Common bean Gene expression Suppression subtractive hybridization Stress-induced transcripts White mold CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Aspectos moleculares da interação patogênica entre Sclerotinia sclerotiorum e Phaseolus vulgaris L. |
title_full |
Aspectos moleculares da interação patogênica entre Sclerotinia sclerotiorum e Phaseolus vulgaris L. |
title_fullStr |
Aspectos moleculares da interação patogênica entre Sclerotinia sclerotiorum e Phaseolus vulgaris L. |
title_full_unstemmed |
Aspectos moleculares da interação patogênica entre Sclerotinia sclerotiorum e Phaseolus vulgaris L. |
title_sort |
Aspectos moleculares da interação patogênica entre Sclerotinia sclerotiorum e Phaseolus vulgaris L. |
author |
Oliveira, Marília Barros |
author_facet |
Oliveira, Marília Barros |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silva, Silvana Petrofeza da |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6823998544968373 |
dc.contributor.referee1.fl_str_mv |
Cunha, Marcos Gomes |
dc.contributor.referee2.fl_str_mv |
Vianello, Rosana Pereira |
dc.contributor.referee3.fl_str_mv |
Melo, Hyrandir Cabral de |
dc.contributor.referee4.fl_str_mv |
Lobo Júnior, Murillo |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7759891178725653 |
dc.contributor.author.fl_str_mv |
Oliveira, Marília Barros |
contributor_str_mv |
Silva, Silvana Petrofeza da Cunha, Marcos Gomes Vianello, Rosana Pereira Melo, Hyrandir Cabral de Lobo Júnior, Murillo |
dc.subject.por.fl_str_mv |
Expressão gênica Feijão comum Hibridização subtrativa por supressão Mofo branco Transcritos induzidos por estresse |
topic |
Expressão gênica Feijão comum Hibridização subtrativa por supressão Mofo branco Transcritos induzidos por estresse Common bean Gene expression Suppression subtractive hybridization Stress-induced transcripts White mold CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Common bean Gene expression Suppression subtractive hybridization Stress-induced transcripts White mold |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
White mold caused by Sclerotinia sclerotiorum (Lib.) De Bary is one of the most devastating diseases of common bean (Phaseolus vulgaris L.) and others crops. This study aimed to achieving a better understanding of the pathosystem S. sclerotiorum-P. vulgaris identifying genes potentially involved in this interaction. Two different approaches were used to evaluate this pathosystem: 1) susceptible cultivar Pérola (BRS) of P. vulgaris; and 2) treatment of this cultivar with the resistance inducer methyl jasmonate (MeJA). For both situations, a suppression subtractive hybridization (SSH) cDNA library approach was used to identify differentially expressed genes in the pathogen and the host plant. In the interaction between the susceptible cultivar and S. sclerotiorum 979 unigenes (430 contigs and 549 singletons) were inentified with 29% of these unigenes were categorized as fungal genes, related to cell wall degradation, proteins degradation, oxidative stress among other. And 58,3% were categorized as plant genes related to energetic metabolism, protein degradation, response to biotic and abiotic stress. The selection of differential clones of their libraries SSH has been validated by reverse transcription and quantitative real-time PCR (RT-qPCR). Were validated genes related to pathogenicity and virulence factors of S. sclerotiorum, such as genes encoding enzymes that degrade plant cell walls (polygalacturonases, cellulases, hemicellulases and proteases). And besides these, defense-related genes of the host plant as: pathogenesis-relateds genes (PR1, PR2, and PR3), phenylpropanoid pathway (Isof, FPS1, and 4CL), and involved in defense and stress-related categories (Lox, Hiprp, GST, POD, and DOX). Exogenous MeJA application induced partial resistance agains S. sclerotiorum in plants as well as a consistent increase in pathogenesis- related protein activities. Furthermore, the validation of SSH made by RT-qPCR showed differential expression of important unigenes over time, such as: pathogenesis related protein PR3 (chitinase class I), PvCallose (callose synthase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like), and a polygalacturonase inhibitor protein (PGIP). The data obtained in this study have highlighted some of the mechanisms involved in the pathogenicity of the fungus, as well as defense responses produced by the plant in two conditions evaluated, thus contributing to a better understanding of pathosystem. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-02-26 |
dc.date.accessioned.fl_str_mv |
2018-12-21T10:11:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, M. B. Aspectos moleculares da interação patogênica entre Sclerotinia sclerotiorum e Phaseolus vulgaris L. 2016. 115 f. Tese (Doutorado em Biologia) - Universidade Federal de Goiás, Goiânia, 2016. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/9170 |
identifier_str_mv |
OLIVEIRA, M. B. Aspectos moleculares da interação patogênica entre Sclerotinia sclerotiorum e Phaseolus vulgaris L. 2016. 115 f. Tese (Doutorado em Biologia) - Universidade Federal de Goiás, Goiânia, 2016. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/9170 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
-3362730732320261554 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
-3872772117827373404 |
dc.relation.cnpq.fl_str_mv |
893477990329266114 |
dc.relation.sponsorship.fl_str_mv |
2075167498588264571 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
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UFG |
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Brasil |
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Instituto de Ciências Biológicas - ICB (RG) |
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Universidade Federal de Goiás |
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Repositório Institucional da UFG |
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Repositório Institucional da UFG |
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Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
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