Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110

Detalhes bibliográficos
Ano de defesa: 2008
Autor(a) principal: SANTOS, Lorena Cristina lattes
Orientador(a): KIPNIS, André lattes
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
dARK ID: ark:/38995/0013000003s1s
Idioma: por
Instituição de defesa: Universidade Federal de Goiás
Programa de Pós-Graduação: Mestrado em Medicina Tropical
Departamento: Medicina
País: BR
Palavras-chave em Português:
Tuberculose
RFLP-IS6110
1. Tuberculose Pacientes - Goiânia(GO) 2. Tuberculose Tratamento. 3. RFLP-IS6110
Palavras-chave em Inglês:
Tuberculosis
RFLP-IS6110
Área do conhecimento CNPq:
CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA
Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tde/1826
Resumo: Tuberculosis is a serious public health problem all over the world. It has been demonstrated that different M. tuberculosis strains, characterized by the RFLP-IS6110 standard technique, have different virulence properties and antibiotic resistance. The aim of this study was to characterize M. tuberculosis strains isolated from patients attending two state reference hospitals of Goiânia-Goiás, using the RFLP-IS6110 technique. Positive cultures of M. tuberculosis sampled and isolated from January 2006 to June 2007 had their DNA extracted. A total of 142 viable DNA samples were analyzed, of which 126 samples presented an RFLP-IS6110 profile. Similarities comparisons between samples, as well as with literature reported profiles, were done with Bionumerics software (version 4.0). Forty three percent of the samples could be grouped in 24 clusters, when analyzed by the RFLP method, suggesting recent transmission among individuals belonging to the same cluster, however those patients did not present any epidemiological relationship, suggesting possible casual transmission between members of the same cluster. When compared with strain profile of the MDR-TB, three samples had grouped in the clade formed by families virulent. This study strengthens the importance of determining transmission routes not only within the state of Goiás but in the entire country, since there is the possibility of resistant strains transmissions.
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spelling KIPNIS, Andréhttp://lattes.cnpq.br/4434965360286741KIPNIS, Ana Paula Junqueirahttp://lattes.cnpq.br/1252262903952987http://lattes.cnpq.br/6790551551363826SANTOS, Lorena Cristina2014-07-29T15:30:41Z2009-08-032008-02-29SANTOS, Lorena Cristina. Molecular characterization of Mycobacterium tuberculosis isolates from patients in the city of Goiânia-GO by the technique of RFLP-IS6110. 2008. 90 f. Dissertação (Mestrado em Medicina) - Universidade Federal de Goiás, Goiânia, 2008.http://repositorio.bc.ufg.br/tede/handle/tde/1826ark:/38995/0013000003s1sTuberculosis is a serious public health problem all over the world. It has been demonstrated that different M. tuberculosis strains, characterized by the RFLP-IS6110 standard technique, have different virulence properties and antibiotic resistance. The aim of this study was to characterize M. tuberculosis strains isolated from patients attending two state reference hospitals of Goiânia-Goiás, using the RFLP-IS6110 technique. Positive cultures of M. tuberculosis sampled and isolated from January 2006 to June 2007 had their DNA extracted. A total of 142 viable DNA samples were analyzed, of which 126 samples presented an RFLP-IS6110 profile. Similarities comparisons between samples, as well as with literature reported profiles, were done with Bionumerics software (version 4.0). Forty three percent of the samples could be grouped in 24 clusters, when analyzed by the RFLP method, suggesting recent transmission among individuals belonging to the same cluster, however those patients did not present any epidemiological relationship, suggesting possible casual transmission between members of the same cluster. When compared with strain profile of the MDR-TB, three samples had grouped in the clade formed by families virulent. This study strengthens the importance of determining transmission routes not only within the state of Goiás but in the entire country, since there is the possibility of resistant strains transmissions.A tuberculose é um grave problema de saúde pública em todo o mundo. Foi demonstrado que diferentes cepas de M. tuberculosis, caracterizadas pela técnica padrão mundial, RFLP-IS6110, possuem diferentes graus de virulência e resistência a antibióticos. O objetivo deste trabalho foi caracterizar as cepas de M. tuberculosis isoladas de pacientes atendidos em dois serviços de referência na cidade de Goiânia-Goiás no período de janeiro de 2006 a junho de 2007, utilizando a técnica RFLP-IS6110. Foram processadas para cultura 175 amostras de escarro com baciloscopias positivas e um total de 142 culturas positivas para M. tuberculosis tiveram seus DNA extraídos, estes foram posteriormente submetidos a técnica de southern blotting para obtenção de seus perfis de bandas de RFLP-IS6110. Destas, 126 amostras apresentaram um perfil de RFLP-IS6110 de fácil comparação de similaridade no programa BioNumerics (versão 4.0). As amostras analisadas pelo RFLP-IS6110 permitiram o agrupamento de 43% das amostras em 24 clusters, sugerindo transmissão recente entre indivíduos agrupados, contudo estes pacientes não apresentaram nenhuma relação epidemiológica, sugerindo possíveis transmissões casuais entre membros de um mesmo cluster. Quando comparamos as amostras com linhagens MDR descritas na literatura, três se agruparam no clado formado pelas famílias virulentas. Este estudo fortaleceu a importância de se traçar cadeias de transmissões bem como a necessidade de se manter um controle de imigrantes e emigrantes visto que há a possibilidade de disseminação de cepas resistentes.application/pdfhttp://repositorio.bc.ufg.br/TEDE/retrieve/4728/Dissertacao%20Lorena%20Cristina.pdf.jpgporUniversidade Federal de GoiásMestrado em Medicina TropicalUFGBRMedicinaTuberculoseRFLP-IS61101. Tuberculose Pacientes - Goiânia(GO) 2. Tuberculose Tratamento. 3. RFLP-IS6110TuberculosisRFLP-IS6110CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIACaracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110Molecular characterization of Mycobacterium tuberculosis isolates from patients in the city of Goiânia-GO by the technique of RFLP-IS6110info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGORIGINALDissertacao Lorena Cristina.pdfapplication/pdf430117http://repositorio.bc.ufg.br/tede/bitstreams/3383921c-5220-4e4c-b511-97c75af1fc9d/download09642b3a4726659137a90dcbf634ba90MD51TEXTDissertacao Lorena Cristina.pdf.txtDissertacao Lorena Cristina.pdf.txtExtracted Texttext/plain113558http://repositorio.bc.ufg.br/tede/bitstreams/d47b035e-e357-4365-83ae-db7bf992806e/downloaddc64cd5455d2cb9ad969195534af6bb5MD52THUMBNAILDissertacao Lorena Cristina.pdf.jpgDissertacao Lorena Cristina.pdf.jpgGenerated Thumbnailimage/jpeg1943http://repositorio.bc.ufg.br/tede/bitstreams/858e7183-928e-4ba9-a795-0fc360b76d31/downloadcc73c4c239a4c332d642ba1e7c7a9fb2MD53tde/18262014-07-30 03:16:08.92open.accessoai:repositorio.bc.ufg.br:tde/1826http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttps://repositorio.bc.ufg.br/tedeserver/oai/requestgrt.bc@ufg.bropendoar:oai:repositorio.bc.ufg.br:tede/12342014-07-30T06:16:08Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)false
dc.title.por.fl_str_mv Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110
dc.title.alternative.eng.fl_str_mv Molecular characterization of Mycobacterium tuberculosis isolates from patients in the city of Goiânia-GO by the technique of RFLP-IS6110
title Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110
spellingShingle Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110
SANTOS, Lorena Cristina
Tuberculose
RFLP-IS6110
1. Tuberculose Pacientes - Goiânia(GO) 2. Tuberculose Tratamento. 3. RFLP-IS6110
Tuberculosis
RFLP-IS6110
CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA
title_short Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110
title_full Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110
title_fullStr Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110
title_full_unstemmed Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110
title_sort Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110
author SANTOS, Lorena Cristina
author_facet SANTOS, Lorena Cristina
author_role author
dc.contributor.advisor1.fl_str_mv KIPNIS, André
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/4434965360286741
dc.contributor.advisor-co1.fl_str_mv KIPNIS, Ana Paula Junqueira
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/1252262903952987
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/6790551551363826
dc.contributor.author.fl_str_mv SANTOS, Lorena Cristina
contributor_str_mv KIPNIS, André
KIPNIS, Ana Paula Junqueira
dc.subject.por.fl_str_mv Tuberculose
RFLP-IS6110
1. Tuberculose Pacientes - Goiânia(GO) 2. Tuberculose Tratamento. 3. RFLP-IS6110
topic Tuberculose
RFLP-IS6110
1. Tuberculose Pacientes - Goiânia(GO) 2. Tuberculose Tratamento. 3. RFLP-IS6110
Tuberculosis
RFLP-IS6110
CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA
dc.subject.eng.fl_str_mv Tuberculosis
RFLP-IS6110
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA
description Tuberculosis is a serious public health problem all over the world. It has been demonstrated that different M. tuberculosis strains, characterized by the RFLP-IS6110 standard technique, have different virulence properties and antibiotic resistance. The aim of this study was to characterize M. tuberculosis strains isolated from patients attending two state reference hospitals of Goiânia-Goiás, using the RFLP-IS6110 technique. Positive cultures of M. tuberculosis sampled and isolated from January 2006 to June 2007 had their DNA extracted. A total of 142 viable DNA samples were analyzed, of which 126 samples presented an RFLP-IS6110 profile. Similarities comparisons between samples, as well as with literature reported profiles, were done with Bionumerics software (version 4.0). Forty three percent of the samples could be grouped in 24 clusters, when analyzed by the RFLP method, suggesting recent transmission among individuals belonging to the same cluster, however those patients did not present any epidemiological relationship, suggesting possible casual transmission between members of the same cluster. When compared with strain profile of the MDR-TB, three samples had grouped in the clade formed by families virulent. This study strengthens the importance of determining transmission routes not only within the state of Goiás but in the entire country, since there is the possibility of resistant strains transmissions.
publishDate 2008
dc.date.issued.fl_str_mv 2008-02-29
dc.date.available.fl_str_mv 2009-08-03
dc.date.accessioned.fl_str_mv 2014-07-29T15:30:41Z
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dc.identifier.citation.fl_str_mv SANTOS, Lorena Cristina. Molecular characterization of Mycobacterium tuberculosis isolates from patients in the city of Goiânia-GO by the technique of RFLP-IS6110. 2008. 90 f. Dissertação (Mestrado em Medicina) - Universidade Federal de Goiás, Goiânia, 2008.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tde/1826
dc.identifier.dark.fl_str_mv ark:/38995/0013000003s1s
identifier_str_mv SANTOS, Lorena Cristina. Molecular characterization of Mycobacterium tuberculosis isolates from patients in the city of Goiânia-GO by the technique of RFLP-IS6110. 2008. 90 f. Dissertação (Mestrado em Medicina) - Universidade Federal de Goiás, Goiânia, 2008.
ark:/38995/0013000003s1s
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dc.publisher.program.fl_str_mv Mestrado em Medicina Tropical
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dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Medicina
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