Avaliação microbiana das manchas dentárias extrínsecas negras em pacientes submetidos a tratamento ortodôntico

• Ano de defesa: 2011
• Autor(a) principal: COSTA, Marília Teixeira
• Orientador(a): PIMENTA, Fabiana Cristina
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Doutorado em Ciencias da Saude
• Departamento: Ciencias da Saude
• País: BR
• Palavras-chave em Português: Manchas dentárias extrínsec negras-paciente-tratamento ortodônt-avaliaç microbiana; Bactérias pigmentadoras de negro; PCR; Manchas dentárias negras; Aparelhos ortodônticos
• Palavras-chave em Inglês: Black pigmented bacteria; PCR; Black extrinsic tooth stains; Orthodontics braces
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tde/1513

Black extrinsic tooth stains appear on the smooth surfaces of teeth, parallel to the marginal gum and are considered a specific form of biofilm due to the high content of calcium, phosphate and insoluble iron salt. This study evaluated the profile of bacterial biofilms in patients with black extrinsic tooth stain with or without fixed metal braces installed. Four bacteria were investigated using conventional multiplex PCR (Polymerase Chain Reaction), black-pigmented bacteria: Prevotella nigrescens and Prevotella intermedia and non pigmented bacteria: Streptococcus mutans and Actinomyces spp. The study enrolled 52 patients, 25 females and 27 males. They were divided into two groups: Group I: 26 patients with black extrinsic tooth stains and Group II: 26 without stains. Twenty patients had fixed orthodontic brackets of stainless steel. The results showed that in both group (I and II) 61.5% of patients had at least one of the bacteria investigated. The P. nigrescens was the most frequently bacteria detected [Group I (30.7%) and Group II (46.1%)], P. intermedia was detected in 3.8% in Group I and 11.5% in Group II. The non pigmented bacteria were found in similar rates in Groups I and II, 19.2% vs 11.5% for Actinomyces spp and 23.1% vs 30.7% for S. mutans. There were no statistically significant differences when comparing the frequencies of each bacterium between groups, indicating that bacterial profiles were similar between patients with or without black extrinsic stains. When analyzed the frequencies of bacteria in the biofilm in black extrinsic tooth stain in patients with braces results indicated that it did not alter the frequency of bacteria found, however, it was observed that the presence of fixed orthodontic appliance significantly reduces the associations between bacteria in the biofilm of black extrinsic stains (p <0.05).