Análise proteômica comparativa entre Paracoccidioides brasiliensis e Paracoccidioides americana

Detalhes bibliográficos
Ano de defesa: 2019
Autor(a) principal: Portis, Igor Godinho lattes
Orientador(a): Soares, Célia Maria de Almeida lattes
Banca de defesa: Soares, Célia Maria de Almeida, Lima, Patrícia de Sousa, Curcio, Juliana Santana De, Pereira, Maristela, Rocha, Juliana Alves Parente
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Goiás
Programa de Pós-Graduação: Programa de Pós-graduação em Genética e Biologia Molecular (ICB)
Departamento: Instituto de Ciências Biológicas - ICB (RG)
País: Brasil
Palavras-chave em Português:
Fungos
Proteínas
Biologia molecular
Molecular biology
Palavras-chave em Inglês:
Fungi
Proteins
Área do conhecimento CNPq:
CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS
Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/10768
Resumo: The genus Paracoccidioides comprises termo dimorphic fungi that cause paracoccidioidomycosis, the most prevalent systemic mycosis in Latin America. These fungi can grow in yeast at 36 ° C and mycelium at temperatures below 28 ° C. Studies have shown that the fungus Paracoccidioides has molecular differences that allow the classification of species. Initially those fungi were classified in a single species, Paracoccidioides brasiliensis, but with the advancement of some molecular and genomic biology techniques, Paracoccidioides is currently classified into five species, P. lutzii, P. brasiliensis, P. americana, P. restrepiensis and P. venezuelensis. However, different species may have different proteomes and cellular responses. In this way in our study, we performed proteomic analyzes of two species of Paracoccidioides, P. americana and P. brasiliensis. iTRAQ labelling and mass spectrometery allowed the identification of 387 statistically significant proteins; two hundreds and two in P. brasiliensis and 111 proteins in P. americana. The identified proteins have different functions, such as amino acid metabolism, energy, cell defense, among others. The results showed differences in protein responses between P. americana and P. brasiliensis in proteins related to cell defense, mitochondria and energy metabolism, suggesting that these fungi may have different metabolism and pathways for growth, profliferation and virulence.
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spelling Soares, Célia Maria de Almeidahttp://lattes.cnpq.br/8539946335852637Ricart, Carlos André Ornelashttp://lattes.cnpq.br/1271167329803156Soares, Célia Maria de AlmeidaLima, Patrícia de SousaCurcio, Juliana Santana DePereira, MaristelaRocha, Juliana Alves Parentehttp://lattes.cnpq.br/7779174594279592Portis, Igor Godinho2020-09-21T11:06:41Z2020-09-21T11:06:41Z2019-12-19PORTIS, I. G. Análise proteômica comparativa entre Paracoccidioides brasiliensis e Paracoccidioides americana. 2019. 160 f. Tese (Doutorado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2019.http://repositorio.bc.ufg.br/tede/handle/tede/10768The genus Paracoccidioides comprises termo dimorphic fungi that cause paracoccidioidomycosis, the most prevalent systemic mycosis in Latin America. These fungi can grow in yeast at 36 ° C and mycelium at temperatures below 28 ° C. Studies have shown that the fungus Paracoccidioides has molecular differences that allow the classification of species. Initially those fungi were classified in a single species, Paracoccidioides brasiliensis, but with the advancement of some molecular and genomic biology techniques, Paracoccidioides is currently classified into five species, P. lutzii, P. brasiliensis, P. americana, P. restrepiensis and P. venezuelensis. However, different species may have different proteomes and cellular responses. In this way in our study, we performed proteomic analyzes of two species of Paracoccidioides, P. americana and P. brasiliensis. iTRAQ labelling and mass spectrometery allowed the identification of 387 statistically significant proteins; two hundreds and two in P. brasiliensis and 111 proteins in P. americana. The identified proteins have different functions, such as amino acid metabolism, energy, cell defense, among others. The results showed differences in protein responses between P. americana and P. brasiliensis in proteins related to cell defense, mitochondria and energy metabolism, suggesting that these fungi may have different metabolism and pathways for growth, profliferation and virulence.O gênero Paracoccidioides compreende fungos termo dimórficos, causadores da paracoccidioidomicose, micose sistêmica mais prevalente da América latina. Esses fungos apresentam capacidade de crescer na forma de levedura a 36°C e na forma de micélio a temperaturas inferiores a 28°C. Estudos mostraram que o fungo Paracoccidioides, possui diferenças moleculares que permitem a sua classificação em espécies. Inicialmente classificava-se apenas em uma única espécie, Paracoccidioides brasiliensis; com o avanço de algumas técnicas de biologia molecular e genômica, o gênero Paracoccidioides é classificado atualmente em cinco espécies, P. lutzii, P. brasiliensis, P. americana, P. restrepiensis e P. venezuelensis. Espécies diferentes podem apresentar proteomas e respostas celulares diferentes; em função disso, em nosso estudo, realizamos as análises proteômicas de duas espécies de Paracoccidioides, P. americana e P. brasiliensis, utilizando a marcação com o reagente iTRAQ e espectrometria de massas. Trezentas e oitenta e sete proteínas foram diferencialmente expressas, sendo duzentos e duas no P. brasiliensis e cento e onze em P. americana. As proteínas identificadas possuem funções diversas, como metabolismo de aminoácidos, energia, defesa celular, entre outras. Os resultados obtidos evidenciam proteínas diferenciais em processos de crescimento, profliferação e virulência.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de GoiásPrograma de Pós-graduação em Genética e Biologia Molecular (ICB)UFGBrasilInstituto de Ciências Biológicas - ICB (RG)Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessFungosProteínasBiologia molecularMolecular biologyFungiProteinsCIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOSAnálise proteômica comparativa entre Paracoccidioides brasiliensis e Paracoccidioides americanaComparative proteomic analysis between Paracoccidioides brasiliensis and Paracoccidioides americanainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis55500500500500236491reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.bc.ufg.br/tede/bitstreams/7bb38fd0-7147-46ee-a8de-72feb04adb18/download8a4605be74aa9ea9d79846c1fba20a33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811http://repositorio.bc.ufg.br/tede/bitstreams/b62cc13b-e453-4797-8a33-c4a70a3a1ce0/downloade39d27027a6cc9cb039ad269a5db8e34MD52ORIGINALTese - Igor Godinho Portis - 2019.pdfTese - Igor Godinho Portis - 2019.pdfapplication/pdf4214800http://repositorio.bc.ufg.br/tede/bitstreams/ef4ce48e-cd97-4193-b2db-ec1df60ae74c/downloadd7e8e6be9ea03de897ef5f7f687b542eMD53tede/107682020-09-21 08:06:41.439http://creativecommons.org/licenses/by-nc-nd/3.0/br/Attribution-NonCommercial-NoDerivs 3.0 Brazilopen.accessoai:repositorio.bc.ufg.br:tede/10768http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttps://repositorio.bc.ufg.br/tedeserver/oai/requestgrt.bc@ufg.bropendoar:oai:repositorio.bc.ufg.br:tede/12342020-09-21T11:06:41Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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
dc.title.pt_BR.fl_str_mv Análise proteômica comparativa entre Paracoccidioides brasiliensis e Paracoccidioides americana
dc.title.alternative.eng.fl_str_mv Comparative proteomic analysis between Paracoccidioides brasiliensis and Paracoccidioides americana
title Análise proteômica comparativa entre Paracoccidioides brasiliensis e Paracoccidioides americana
spellingShingle Análise proteômica comparativa entre Paracoccidioides brasiliensis e Paracoccidioides americana
Portis, Igor Godinho
Fungos
Proteínas
Biologia molecular
Molecular biology
Fungi
Proteins
CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS
title_short Análise proteômica comparativa entre Paracoccidioides brasiliensis e Paracoccidioides americana
title_full Análise proteômica comparativa entre Paracoccidioides brasiliensis e Paracoccidioides americana
title_fullStr Análise proteômica comparativa entre Paracoccidioides brasiliensis e Paracoccidioides americana
title_full_unstemmed Análise proteômica comparativa entre Paracoccidioides brasiliensis e Paracoccidioides americana
title_sort Análise proteômica comparativa entre Paracoccidioides brasiliensis e Paracoccidioides americana
author Portis, Igor Godinho
author_facet Portis, Igor Godinho
author_role author
dc.contributor.advisor1.fl_str_mv Soares, Célia Maria de Almeida
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/8539946335852637
dc.contributor.advisor-co1.fl_str_mv Ricart, Carlos André Ornelas
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/1271167329803156
dc.contributor.referee1.fl_str_mv Soares, Célia Maria de Almeida
dc.contributor.referee2.fl_str_mv Lima, Patrícia de Sousa
dc.contributor.referee3.fl_str_mv Curcio, Juliana Santana De
dc.contributor.referee4.fl_str_mv Pereira, Maristela
dc.contributor.referee5.fl_str_mv Rocha, Juliana Alves Parente
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7779174594279592
dc.contributor.author.fl_str_mv Portis, Igor Godinho
contributor_str_mv Soares, Célia Maria de Almeida
Ricart, Carlos André Ornelas
Soares, Célia Maria de Almeida
Lima, Patrícia de Sousa
Curcio, Juliana Santana De
Pereira, Maristela
Rocha, Juliana Alves Parente
dc.subject.por.fl_str_mv Fungos
Proteínas
Biologia molecular
Molecular biology
topic Fungos
Proteínas
Biologia molecular
Molecular biology
Fungi
Proteins
CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS
dc.subject.eng.fl_str_mv Fungi
Proteins
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS
description The genus Paracoccidioides comprises termo dimorphic fungi that cause paracoccidioidomycosis, the most prevalent systemic mycosis in Latin America. These fungi can grow in yeast at 36 ° C and mycelium at temperatures below 28 ° C. Studies have shown that the fungus Paracoccidioides has molecular differences that allow the classification of species. Initially those fungi were classified in a single species, Paracoccidioides brasiliensis, but with the advancement of some molecular and genomic biology techniques, Paracoccidioides is currently classified into five species, P. lutzii, P. brasiliensis, P. americana, P. restrepiensis and P. venezuelensis. However, different species may have different proteomes and cellular responses. In this way in our study, we performed proteomic analyzes of two species of Paracoccidioides, P. americana and P. brasiliensis. iTRAQ labelling and mass spectrometery allowed the identification of 387 statistically significant proteins; two hundreds and two in P. brasiliensis and 111 proteins in P. americana. The identified proteins have different functions, such as amino acid metabolism, energy, cell defense, among others. The results showed differences in protein responses between P. americana and P. brasiliensis in proteins related to cell defense, mitochondria and energy metabolism, suggesting that these fungi may have different metabolism and pathways for growth, profliferation and virulence.
publishDate 2019
dc.date.issued.fl_str_mv 2019-12-19
dc.date.accessioned.fl_str_mv 2020-09-21T11:06:41Z
dc.date.available.fl_str_mv 2020-09-21T11:06:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
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dc.identifier.citation.fl_str_mv PORTIS, I. G. Análise proteômica comparativa entre Paracoccidioides brasiliensis e Paracoccidioides americana. 2019. 160 f. Tese (Doutorado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2019.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/10768
identifier_str_mv PORTIS, I. G. Análise proteômica comparativa entre Paracoccidioides brasiliensis e Paracoccidioides americana. 2019. 160 f. Tese (Doutorado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2019.
url http://repositorio.bc.ufg.br/tede/handle/tede/10768
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http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
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dc.publisher.none.fl_str_mv Universidade Federal de Goiás
dc.publisher.program.fl_str_mv Programa de Pós-graduação em Genética e Biologia Molecular (ICB)
dc.publisher.initials.fl_str_mv UFG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Instituto de Ciências Biológicas - ICB (RG)
publisher.none.fl_str_mv Universidade Federal de Goiás
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