Avaliação bioquímica in vivo do tratamento anti-helmíntico de cisticercos de Taenia crassiceps

• Ano de defesa: 2011
• Autor(a) principal: Fraga, Carolina Miguel
• Orientador(a): Vinaud, Marina Clare
• Banca de defesa: Vinaud, Marina Clare, Oliveira, Valéria de, Bezerra, José Clecildo Barreto
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)
• Departamento: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
• País: Brasil
• Palavras-chave em Português: Taenia crassiceps; Tratamento in vivo; Metabolismo energético; Metabolismo respiratório; Ácidos orgânicos
• Palavras-chave em Inglês: In vivo treatment; Energetic metabolism; Respiratory metabolism; Organic acids
• Área do conhecimento CNPq: CIENCIAS BIOLOGICAS::PARASITOLOGIA
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/10471

Taenia crassiceps cysticercosis is a rare disease in humans and domestic animals, however there are reports of cases of humans as paratenic hosts, especially immunocompromised ones which indicates this parasite as of zoonotic risk. Albendazole and praziquantel are widely used drugs in cysticercosis treatment as they are well tolerated by patients. Biochemically, the active forms of albendazole inhibit the glucose uptake which leads to energy production breakdown and death of the parasite. The active forms of praziquantel induce muscle contractions, tegument damages and metabolic alterations. Throughout the life cycle, the energetic metabolism of T. crassiceps varies accordingly to nutrient sources and oxygen tension. Cysticerci use glucose and glycogen as energy source and reserve substances, respectively, and the products generated from their degradation are used in other metabolic pathways. Besides, the parasite is capable of fatty acid oxidation and amino acids breakdown as alternative energy production sources. The aim of this study was to evaluate the biochemical alterations, in vivo, in T. crassiceps cysticerci after treatment with low doses of albendazole and praziquantel. The cysticerci were inoculated into the peritoneal cavity of female BALB/c mice which after 30 days of infection received treatment with one of the following dosages: 5,75 or 11,5 mg/kg of albendazole or 3,83 or 7,67 mg/kg of praziquantel. After 24h the animals were euthanized and the cysticerci removed and classified as belonging to initial, larval or final stage accordingly to their morphologic characteristics. The cysticerci were analyzed through high performance liquid chromatography aiming the detection of organic acids from glycolisis, tricarboxylic acid cycle and fatty acid oxidation, and through spectrophotometry aiming the quantification of glucose, urea and creatinine. The low dosage treatment induced a partial blockage of the glucose uptake by the cysticerci. The concentrations of the drugs used were capable of activation of the tricarboxylic acid cycle which are considered energetically more profitable which was reflected by the increase in the concentrations of citrate, malate and α-cetoglutarato, which was reported in cysticerci for the first time. The used dosages of the drugs induced alterations in the parasite’s metabolism increasing the use of alternative energy production pathways such as the fatty acid oxidation, detected by the presence of propionate and β-hydroxibutyrate, and the aminoacids breakdown, detected by the urea production.