Nova abordagem para análise de neurotransmissores em tecidos cerebrais por DESI-imaging
Ano de defesa: | 2021 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Goiás
|
Programa de Pós-Graduação: |
Programa de Pós-graduação em Química (IQ)
|
Departamento: |
Instituto de Química - IQ (RG)
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.bc.ufg.br/tede/handle/tede/11217 |
Resumo: | Mass spectrometry imaging using desorption electrospray ionization (DESI-imaging) is a method with the potential to be applied to investigate the distribution of neurotransmitters (biogenic amines and amino acids) in brain tissues. Despite the low ionization efficiency of neurotransmitters in direct tissue analysis by DESI, due to signal suppression by lipids, neurotransmitters can be analyzed using derivatization protocols that insert a known compound into their structures through a chemical reaction. In this sense, this work aims to apply chemical derivatization protocols in brain tissues to be analyzed by DESI-imaging. The derivatization used the 2,4,6-triphenylpyrylium tetrafluoroborate (TPP+) reagent and was first investigated in a solvent in order to ensure the detection of the expected products. Subsequently, the brain tissue surface was subjected to the derivatization reaction through the application of TPP+ by spraying. Then, the tissue was analyzed by DESI, and the ions derived from glycine (Gly), alanine (Ala), dopamine (DOPA), and gamma-aminobutyric acid (GABA) were detected. In addition, the DESI-imaging analysis demonstrated distinct spatial distributions for these derivatized neurotransmitters. This protocol was applied in the study of neurotransmitters in mice with Parkinson's, and it was possible to verify differences in the intensities and anatomical distribution for these derivatives in control and diseased (affected by Parkinson's disease model) tissues. Thus, the derivatization/DESI-imaging protocol described in this work emerges as a potential tool for analyzing Parkinson's Disease biomarkers in mouse brain tissues. |
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Vaz, Boniek Gontijohttp://lattes.cnpq.br/7814534710550639Pinto, Mauro Cunha Xavierhttp://lattes.cnpq.br/0868250984727943Vaz, Boniek GontijoAbdelnur, Patrícia VerardiPorcari, Andréia de Melohttp://lattes.cnpq.br/6937001124096815Maciel, Lanaia Ítala Louzeiro2021-03-31T14:24:14Z2021-03-31T14:24:14Z2021-02-08MACIEL , L. Í. L. Nova abordagem para análise de neurotransmissores em tecidos cerebrais por DESI-imaging. 2021. 75 f. Dissertação (Mestrado em Química) - Universidade Federal de Goiás, Goiânia, 2021.http://repositorio.bc.ufg.br/tede/handle/tede/11217Mass spectrometry imaging using desorption electrospray ionization (DESI-imaging) is a method with the potential to be applied to investigate the distribution of neurotransmitters (biogenic amines and amino acids) in brain tissues. Despite the low ionization efficiency of neurotransmitters in direct tissue analysis by DESI, due to signal suppression by lipids, neurotransmitters can be analyzed using derivatization protocols that insert a known compound into their structures through a chemical reaction. In this sense, this work aims to apply chemical derivatization protocols in brain tissues to be analyzed by DESI-imaging. The derivatization used the 2,4,6-triphenylpyrylium tetrafluoroborate (TPP+) reagent and was first investigated in a solvent in order to ensure the detection of the expected products. Subsequently, the brain tissue surface was subjected to the derivatization reaction through the application of TPP+ by spraying. Then, the tissue was analyzed by DESI, and the ions derived from glycine (Gly), alanine (Ala), dopamine (DOPA), and gamma-aminobutyric acid (GABA) were detected. In addition, the DESI-imaging analysis demonstrated distinct spatial distributions for these derivatized neurotransmitters. This protocol was applied in the study of neurotransmitters in mice with Parkinson's, and it was possible to verify differences in the intensities and anatomical distribution for these derivatives in control and diseased (affected by Parkinson's disease model) tissues. Thus, the derivatization/DESI-imaging protocol described in this work emerges as a potential tool for analyzing Parkinson's Disease biomarkers in mouse brain tissues.O imageamento por espectrometria de massas utilizando a desorption electrospray ionization (DESI-imaging) é um método com potencial para ser aplicado na investigação da distribuição de neurotransmissores (aminas biogênicas e aminoácidos) em tecidos cerebrais. Apesar da baixa eficiência de ionização dos neurotransmissores em análises diretas de tecidos por DESI, em virtude do efeito de supressão de sinal pelos lipídeos, os neurotransmissores podem ser analisados utilizando protocolos de derivatização que inserem um composto conhecido em suas estruturas através de uma reação química. Nesse sentido, este trabalho tem como objetivo a aplicação de protocolos de derivatização química em tecidos cerebrais para análises por DESI-imaging. A derivatização empregada neste estudo utiliza o reagente tetrafluoroborato de 2,4,6 trifenilpirílio (TPP+), e foi primeiramente investigada em solvente com o intuito de garantir a detecção dos produtos esperados. Posteriormente, a superfície do tecido cerebral foi submetida à reação de derivatização através da aplicação do TPP+ em forma de spray com o auxílio de um pulverizador. Em seguida, o tecido foi submetido à análise por DESI, sendo possível detectar íons derivados de glicina (Gly), alanina (Ala), dopamina (DOPA) e ácido gama- aminobutírico (GABA). Além disso, os estudos de imageamento químico por DESI-imaging demonstraram distribuições espaciais distintas para estes neurotransmissores derivatizados. O presente protocolo foi aplicado ao modelo de camundongos da Doença de Parkinson, no qual, foi possível verificar diferenças de intensidades e distribuição anatômica para esses derivados em tecidos controles e doentes (submetidos ao modelo). Deste modo, o protocolo de derivatização/DESI-imaging descrito neste trabalho emerge como uma ferramenta em potencial para análise de biomarcadores da Doença de Parkinson em tecidos de cerebrais de camundongos.Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2021-03-30T21:31:06Z No. of bitstreams: 2 Dissertação - Lanaia Ítala Louzeiro Maciel - 2021.pdf: 3267695 bytes, checksum: fa9387b0db068cde830521dc36bee716 (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2021-03-31T14:24:14Z (GMT) No. of bitstreams: 2 Dissertação - Lanaia Ítala Louzeiro Maciel - 2021.pdf: 3267695 bytes, checksum: fa9387b0db068cde830521dc36bee716 (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5)Made available in DSpace on 2021-03-31T14:24:14Z (GMT). No. of bitstreams: 2 Dissertação - Lanaia Ítala Louzeiro Maciel - 2021.pdf: 3267695 bytes, checksum: fa9387b0db068cde830521dc36bee716 (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5) Previous issue date: 2021-02-08Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de GoiásPrograma de Pós-graduação em Química (IQ)UFGBrasilInstituto de Química - IQ (RG)Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessImageamento químicoChemical imagingCIENCIAS EXATAS E DA TERRA::QUIMICA::FISICO-QUIMICANova abordagem para análise de neurotransmissores em tecidos cerebrais por DESI-imagingA new approach for analysis of neurotransmitters in brain tissue by DESI-imaginginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis805005005005002910921reponame:Biblioteca Digital de Teses e Dissertações da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGORIGINALDissertação - Lanaia Ítala Louzeiro Maciel - 2021.pdfDissertação - Lanaia Ítala Louzeiro Maciel - 2021.pdfapplication/pdf3267695http://repositorio.bc.ufg.br/tede/bitstreams/d923bfeb-d3b8-40c5-bdc9-13dfe2b4230e/downloadfa9387b0db068cde830521dc36bee716MD53LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.bc.ufg.br/tede/bitstreams/0788f427-d097-40c1-843a-c80486f413d8/download8a4605be74aa9ea9d79846c1fba20a33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.bc.ufg.br/tede/bitstreams/e07da697-b6bd-4ca9-9424-79cf8dc42cf9/download4460e5956bc1d1639be9ae6146a50347MD52tede/112172021-03-31 11:24:14.943http://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internationalopen.accessoai:repositorio.bc.ufg.br:tede/11217http://repositorio.bc.ufg.br/tedeBiblioteca Digital de Teses e Dissertaçõeshttp://repositorio.bc.ufg.br/PUBhttps://repositorio.bc.ufg.br/tede_oai/requesttesesdissertacoes.bc@ufg.br ||tesesdissertacoes.bc@ufg.bropendoar:32082021-03-31T14:24:14Biblioteca Digital de Teses e Dissertações da UFG - Universidade Federal de Goiás (UFG)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 |
dc.title.pt_BR.fl_str_mv |
Nova abordagem para análise de neurotransmissores em tecidos cerebrais por DESI-imaging |
dc.title.alternative.eng.fl_str_mv |
A new approach for analysis of neurotransmitters in brain tissue by DESI-imaging |
title |
Nova abordagem para análise de neurotransmissores em tecidos cerebrais por DESI-imaging |
spellingShingle |
Nova abordagem para análise de neurotransmissores em tecidos cerebrais por DESI-imaging Maciel, Lanaia Ítala Louzeiro Imageamento químico Chemical imaging CIENCIAS EXATAS E DA TERRA::QUIMICA::FISICO-QUIMICA |
title_short |
Nova abordagem para análise de neurotransmissores em tecidos cerebrais por DESI-imaging |
title_full |
Nova abordagem para análise de neurotransmissores em tecidos cerebrais por DESI-imaging |
title_fullStr |
Nova abordagem para análise de neurotransmissores em tecidos cerebrais por DESI-imaging |
title_full_unstemmed |
Nova abordagem para análise de neurotransmissores em tecidos cerebrais por DESI-imaging |
title_sort |
Nova abordagem para análise de neurotransmissores em tecidos cerebrais por DESI-imaging |
author |
Maciel, Lanaia Ítala Louzeiro |
author_facet |
Maciel, Lanaia Ítala Louzeiro |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Vaz, Boniek Gontijo |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7814534710550639 |
dc.contributor.advisor-co1.fl_str_mv |
Pinto, Mauro Cunha Xavier |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://lattes.cnpq.br/0868250984727943 |
dc.contributor.referee1.fl_str_mv |
Vaz, Boniek Gontijo |
dc.contributor.referee2.fl_str_mv |
Abdelnur, Patrícia Verardi |
dc.contributor.referee3.fl_str_mv |
Porcari, Andréia de Melo |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6937001124096815 |
dc.contributor.author.fl_str_mv |
Maciel, Lanaia Ítala Louzeiro |
contributor_str_mv |
Vaz, Boniek Gontijo Pinto, Mauro Cunha Xavier Vaz, Boniek Gontijo Abdelnur, Patrícia Verardi Porcari, Andréia de Melo |
dc.subject.por.fl_str_mv |
Imageamento químico |
topic |
Imageamento químico Chemical imaging CIENCIAS EXATAS E DA TERRA::QUIMICA::FISICO-QUIMICA |
dc.subject.eng.fl_str_mv |
Chemical imaging |
dc.subject.cnpq.fl_str_mv |
CIENCIAS EXATAS E DA TERRA::QUIMICA::FISICO-QUIMICA |
description |
Mass spectrometry imaging using desorption electrospray ionization (DESI-imaging) is a method with the potential to be applied to investigate the distribution of neurotransmitters (biogenic amines and amino acids) in brain tissues. Despite the low ionization efficiency of neurotransmitters in direct tissue analysis by DESI, due to signal suppression by lipids, neurotransmitters can be analyzed using derivatization protocols that insert a known compound into their structures through a chemical reaction. In this sense, this work aims to apply chemical derivatization protocols in brain tissues to be analyzed by DESI-imaging. The derivatization used the 2,4,6-triphenylpyrylium tetrafluoroborate (TPP+) reagent and was first investigated in a solvent in order to ensure the detection of the expected products. Subsequently, the brain tissue surface was subjected to the derivatization reaction through the application of TPP+ by spraying. Then, the tissue was analyzed by DESI, and the ions derived from glycine (Gly), alanine (Ala), dopamine (DOPA), and gamma-aminobutyric acid (GABA) were detected. In addition, the DESI-imaging analysis demonstrated distinct spatial distributions for these derivatized neurotransmitters. This protocol was applied in the study of neurotransmitters in mice with Parkinson's, and it was possible to verify differences in the intensities and anatomical distribution for these derivatives in control and diseased (affected by Parkinson's disease model) tissues. Thus, the derivatization/DESI-imaging protocol described in this work emerges as a potential tool for analyzing Parkinson's Disease biomarkers in mouse brain tissues. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-03-31T14:24:14Z |
dc.date.available.fl_str_mv |
2021-03-31T14:24:14Z |
dc.date.issued.fl_str_mv |
2021-02-08 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MACIEL , L. Í. L. Nova abordagem para análise de neurotransmissores em tecidos cerebrais por DESI-imaging. 2021. 75 f. Dissertação (Mestrado em Química) - Universidade Federal de Goiás, Goiânia, 2021. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/11217 |
identifier_str_mv |
MACIEL , L. Í. L. Nova abordagem para análise de neurotransmissores em tecidos cerebrais por DESI-imaging. 2021. 75 f. Dissertação (Mestrado em Química) - Universidade Federal de Goiás, Goiânia, 2021. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/11217 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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80 |
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500 500 500 500 |
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29 |
dc.relation.cnpq.fl_str_mv |
1092 |
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1 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Química (IQ) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Química - IQ (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
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