Avaliação da composição química de indivíduos adultos de araucaria angustifolia (Bert.) kutz. através da RMN aliada a quimiometria visando a distinção sexual

• Ano de defesa: 2016
• Autor(a) principal: Oliveira, Gerlon de Almeida Ribeiro
• Orientador(a): Lião, Luciano Morais
• Banca de defesa: Lião, Luciano Morais, Barison, Andersson, Novotny, Etelvino Henrique, Zanette, Flávio, Venturoli, Fábio
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Química (IQ)
• Departamento: Instituto de Química - IQ (RG)
• País: Brasil
• Palavras-chave em Português: Araucaria; Discriminação sexual; RMN; Espectrometria de massas; Infravermelho
• Palavras-chave em Inglês: Sexual discrimination; NMR; Mass spectrometry; Infrared spectroscopy
• Área do conhecimento CNPq: CIENCIAS EXATAS E DA TERRA::QUIMICA::FISICO-QUIMICA
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/12109

Araucaria angustifolia, popularly known as araucaria or pine of Paraná, is an endemic conifer in the Brazilian southern and southeastern. The preserved areas represent source of income to a great number of families of collectors, due to the interest of the population for the so called “pinhão”, the seed of the araucaria. Araucaria is a dioecious plant, and only the female plants produce the “pinhão”. Determining the sex of the seedlings would be interesting so that a higher number of females than males could be planted, which would increase the production of pinhão and would make this species more attractive for reforestation purposes. There is no technique for knowing the sex of the seedlings before they express their sexuality, which occurs between fifteen and twenty years after planting. The objective of this study was to evaluate the possibility of using nuclear magnetic resonance (NMR), mass spectrometry (MS), and infrared spectroscopy in the non-targeted metabolomic approach for the sexual determination of adult individuals of araucaria. 80 samples of adult plant needles were collected in the state of Paraná, Brazil. The needles were macerated with liquid nitrogen and extracted with deuterated chloroform (NMR), or methanol (MS). Several pre-treatments were evaluated, as well as different non-supervised (clusters analysis and principal component analysis, PCA) and supervised multivariate techniques (partial least squares discriminant analysis – PLS-DA, linear discriminant analysis, LDA, after selection of variables by genetic algorithm). Using the strategy described herein, the chemometric models indicated only some trends of separation of the groups according to sex, which, however, were not generalizable.