Toxicidade de aditivos e de formulados de Metarhizium anisopliae s.I. IP46 em adultos de Aedes aegypti e conidiogênese quantitativa na fase saprobiôntica
Ano de defesa: | 2012 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Goiás
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Programa de Pós-Graduação: |
Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)
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Departamento: |
Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
|
País: |
Brasil
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Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.bc.ufg.br/tede/handle/tede/9277 |
Resumo: | Aedes aegypti is both the dengue and urban yellow fever vector in Brazil. Elimination of this mosquito is a strategy taken for the control of dengue. Entomopathogenic fungi such as Metarhizium anisopliae exhibit ovicidal, larvicidal and adulticidal activity on A. aegypti, being therefore of great interest for the integrated control of such mosquito. Water based, oil based, water/oil/powder based fungal formulations may help such fungi overtake environmental adverse effects as well as magnify their entomopathogenic action. Nevertheless, little is known about the effect of additives on A. aegypti adults. This study reports the effect of additives such as diatomaceous earth and oils (both vegetal and mineral), with or without the presence of M. anisopliae conidia IP 46 on A. aegypti adult mosquitoes, the effect of exposure time on accumulated mortality of such mosquitoes as well as the effects of formulations on conidiogenesis on dead adult mosquitoes killed by infection. Graxol ® vegetable oil was tested in two concentrations (0.25 and 0.05 µl/cm 2 ), as well as Naturol ® mineral oil (0,25 µl/cm 2 ) and KeepDry ® diatomaceous earth (0,6 mg/cm 2 ). Such additives were analyzed both separately and in combination (KeepDry ® + Graxol ® ; KeepDry ® + Naturol ® ). Subsequently, oily formulations or combinations of IP 46 at 3.3 x 10 6 conidia/cm 2 + KeepDry ® + oils (Graxol ® or Naturol ® ) were tested. Additives and formulations were applied inside plastic cups. Ten A. aegypti adults were placed in the cup and kept at 25°C, relative humidity of 75% and 12 hours of photophasis with permanent access to a 10% sugar solution. In order to assess the effect of the time of exposure, mosquitoes were exposed to IP 46 conidia (3.3 x 10 6 conidia/cm 2 ) formulations on diatomaceous earth and mineral oil for 0.016 hours (60s), 12 hours, 48 hours, 72 hours and 120 hours. A non-treated control group was kept at the same conditions. Mortality was daily verified for 15 days. Mosquitoes killed by fungal infection were incubated for 10 days in a humid chamber with relative humidity close to saturation (≥ 98%) and then suspended in Tween 80 ® (0,1%) for conidia quantification in Neubauer chamber. Results showed that the additives exert toxicity on A. aegypti adults as well as effect on mortality (F 6 , 35 = 31,9, P = 0,0001). IP 46 conidia combined to pure KeepDry ® or on oil associations took shorter to kill 50% (TL 50 ) of mosquitoes and the IP 46 + KD + N combination took less time to kill 90% (TL 90 ), namely 7.5 days. There was no relevant difference between time of exposure on accumulated mortality of adults exposed to combined and non-combined IP 46conidia. The growth of mycelia on dead adults started within 2 days of humid chamber incubation. New IP 46 conidia where observed from the 3 rd day on, regardless conidia concentration and combination type to which mosquitoes had previously been exposed to. The effect of the combination on quantitative conidiogenesis on dead mosquitoes was significantly different (F 5,18 = 3.8; P = 0.02), a greater number of conidia on dead mosquitoes being found on mosquitoes who were exposed to the IP 46 + KeepDry ® combination. The IP 46 with KeepDry ® and Naturol ® combination proved to be the most recommended one for the control of A. aegypti adults using a mycoinsecticide. |
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Luz, Wolf Christianhttp://lattes.cnpq.br/1104009511235835Fernandes, Éverton Kot Kamphttp://lattes.cnpq.br/2135541732341157Luz, Wolf ChristianSantos, Solange Xavier dosRocha, Luiz Fernando Nuneshttp://lattes.cnpq.br/8824033277119685Borges, Priscilla Rodrigues2019-02-07T10:41:46Z2012-02-15BORGES, P. R. Toxicidade de aditivos e de formulados de Metarhizium anisopliae s.I. IP46 em adultos de Aedes aegypti e conidiogênese quantitativa na fase saprobiôntica. 2012. 51 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2012.http://repositorio.bc.ufg.br/tede/handle/tede/9277Aedes aegypti is both the dengue and urban yellow fever vector in Brazil. Elimination of this mosquito is a strategy taken for the control of dengue. Entomopathogenic fungi such as Metarhizium anisopliae exhibit ovicidal, larvicidal and adulticidal activity on A. aegypti, being therefore of great interest for the integrated control of such mosquito. Water based, oil based, water/oil/powder based fungal formulations may help such fungi overtake environmental adverse effects as well as magnify their entomopathogenic action. Nevertheless, little is known about the effect of additives on A. aegypti adults. This study reports the effect of additives such as diatomaceous earth and oils (both vegetal and mineral), with or without the presence of M. anisopliae conidia IP 46 on A. aegypti adult mosquitoes, the effect of exposure time on accumulated mortality of such mosquitoes as well as the effects of formulations on conidiogenesis on dead adult mosquitoes killed by infection. Graxol ® vegetable oil was tested in two concentrations (0.25 and 0.05 µl/cm 2 ), as well as Naturol ® mineral oil (0,25 µl/cm 2 ) and KeepDry ® diatomaceous earth (0,6 mg/cm 2 ). Such additives were analyzed both separately and in combination (KeepDry ® + Graxol ® ; KeepDry ® + Naturol ® ). Subsequently, oily formulations or combinations of IP 46 at 3.3 x 10 6 conidia/cm 2 + KeepDry ® + oils (Graxol ® or Naturol ® ) were tested. Additives and formulations were applied inside plastic cups. Ten A. aegypti adults were placed in the cup and kept at 25°C, relative humidity of 75% and 12 hours of photophasis with permanent access to a 10% sugar solution. In order to assess the effect of the time of exposure, mosquitoes were exposed to IP 46 conidia (3.3 x 10 6 conidia/cm 2 ) formulations on diatomaceous earth and mineral oil for 0.016 hours (60s), 12 hours, 48 hours, 72 hours and 120 hours. A non-treated control group was kept at the same conditions. Mortality was daily verified for 15 days. Mosquitoes killed by fungal infection were incubated for 10 days in a humid chamber with relative humidity close to saturation (≥ 98%) and then suspended in Tween 80 ® (0,1%) for conidia quantification in Neubauer chamber. Results showed that the additives exert toxicity on A. aegypti adults as well as effect on mortality (F 6 , 35 = 31,9, P = 0,0001). IP 46 conidia combined to pure KeepDry ® or on oil associations took shorter to kill 50% (TL 50 ) of mosquitoes and the IP 46 + KD + N combination took less time to kill 90% (TL 90 ), namely 7.5 days. There was no relevant difference between time of exposure on accumulated mortality of adults exposed to combined and non-combined IP 46conidia. The growth of mycelia on dead adults started within 2 days of humid chamber incubation. New IP 46 conidia where observed from the 3 rd day on, regardless conidia concentration and combination type to which mosquitoes had previously been exposed to. The effect of the combination on quantitative conidiogenesis on dead mosquitoes was significantly different (F 5,18 = 3.8; P = 0.02), a greater number of conidia on dead mosquitoes being found on mosquitoes who were exposed to the IP 46 + KeepDry ® combination. The IP 46 with KeepDry ® and Naturol ® combination proved to be the most recommended one for the control of A. aegypti adults using a mycoinsecticide.Aedes aegypti é o vetor da dengue e da febre amarela urbana no Brasil. A eliminação deste mosquito é uma estratégia para o controle da dengue, e medidas como o controle integrado, tem sido adotadas. Fungos entomopatogênicos como Metarhizium anisopliae possuem atividade ovicida, larvicida e adulticida em A. aegypti, por isso, são de grande interesse para um controle deste mosquito. Formulações fúngicas à base de água, óleo, água-óleo e pó, podem auxiliar o fungo na superação de efeitos adversos no ambiente e potencializar sua ação entomopatogênica. Entretanto, pouco se sabe sobre o efeito de aditivos nas formulações em adultos de A. aegypti. O presente estudo relata o efeito de terra diatomácea e óleos (vegetal e mineral), formulados ou não com conídios de M. anisopliae IP 46 em adultos de A. aegypti, o efeito de diferentes tempos de exposição sobre a mortalidade acumulada destes mosquitos e o efeito de formulados sobre conidiogênese quantitativa em adultos mortos por infecção. Foram testados óleo vegetal Graxol ® em duas concentrações (0,25 e 0,05 µl/cm 2 ), óleo mineral Naturol ® a 0,25 µl/cm 2 e terra diatomácea KeepDry ® a 0,6 mg/cm 2 . Primeiramente os aditivos foram avaliados separadamente e também em combinações (KeepDry ® + Graxol ® ; KeepDry ® + Naturol ® ). Posteriormente formulações oleosas ou combinações de IP 46 a 3,3 x 10 6 conídios/cm 2 + KeepDry ® + óleos (Graxol ® ou Naturol ® ) foram testadas. Aditivos e formulações foram aplicados na área interna de copos plásticos. Dez adultos de A aegypti foram colocados no copo e mantidos a 25°C, 75% de umidade relativa e fotofase de 12 h com acesso permanente a solução açucarada a 10%. Para avaliar o efeito do tempo de exposição, mosquitos foram expostos a conídios de IP 46 (3,3 x 10 6 conídios/cm 2 ) formulados em terra diatomácea e óleo mineral durante 0,016 h (60s), 12 h, 48 h, 72 h e 120 h. Um grupo controle sem tratamento foi mantido nas mesmas condições. A mortalidade foi avaliada, diariamente, por 15 dias. Mosquitos mortos por infecção fúngica foram incubados por 10 dias em câmara úmida com umidades relativas perto de saturação (≥ 98%) e em seguida suspensos em Tween 80 ® (0,1%) para quantificação de conídios em câmara de Neubauer. Os resultados mostraram que os aditivos possuem toxicidade para adultos de A. aegypti e efeito sobre a mortalidade acumulada (F 6 , 35 = 31,9, P = 0,0001). Conídios de IP 46 combinados com KeepDry ® puro ou em associações com óleos apresentaram menor tempo para matar 50% (TL 50 ) dos mosquitos e o formulado IP 46 + KD + N, levou menos tempo para matar 90% (TL 90 ) que foi 7,5 dias. Não houve diferença significativa do tempo de exposição sobre a mortalidade acumulada de adultos expostos a conídios de IP 46 formulados ou não. O crescimento de micélio sobre adultos mortos teve início com 2 dias de incubação em câmara úmida. Novos conídios de IP 46 foram visualizados a partir do 3° dia independentemente da concentração de conídios e do tipo de formulado em que os mosquitos tinham sido expostos anteriormente. O efeito do formualado sobre a conidiogênese quantitativa em mosquitos mortos foi significativamente diferente (F 5,18 = 3,8; P = 0,02), sendo encontrado maior número de conídios em mosquitos que foram expostos ao formulado IP 46 + KeepDry ® . A combinação de conídios de IP 46 com KeepDry ® e Naturol ® , mostrou-se a mais indicada para um controle de adultos A. aegypti com um micoinseticida.Submitted by Ana Caroline Costa (ana_caroline212@hotmail.com) on 2019-02-06T19:16:58Z No. of bitstreams: 2 Dissertação - Priscilla Rodrigues Borges - 2012.pdf: 2626312 bytes, checksum: d4d0afa453a704040a0ddde6c8e96533 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2019-02-07T10:41:46Z (GMT) No. of bitstreams: 2 Dissertação - Priscilla Rodrigues Borges - 2012.pdf: 2626312 bytes, checksum: d4d0afa453a704040a0ddde6c8e96533 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2019-02-07T10:41:46Z (GMT). No. of bitstreams: 2 Dissertação - Priscilla Rodrigues Borges - 2012.pdf: 2626312 bytes, checksum: d4d0afa453a704040a0ddde6c8e96533 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2012-02-15Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)UFGBrasilInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessFungos entomopatogênicosControle de Aedes aegyptiToxicidade de aditivosDiferentes tempos de formulaçãoEntomopathogenic fungiControl of Aedes aegyptiToxicity of additivesDifferent formulation timesCIENCIAS BIOLOGICAS::PARASITOLOGIAToxicidade de aditivos e de formulados de Metarhizium anisopliae s.I. 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dc.title.eng.fl_str_mv |
Toxicidade de aditivos e de formulados de Metarhizium anisopliae s.I. IP46 em adultos de Aedes aegypti e conidiogênese quantitativa na fase saprobiôntica |
title |
Toxicidade de aditivos e de formulados de Metarhizium anisopliae s.I. IP46 em adultos de Aedes aegypti e conidiogênese quantitativa na fase saprobiôntica |
spellingShingle |
Toxicidade de aditivos e de formulados de Metarhizium anisopliae s.I. IP46 em adultos de Aedes aegypti e conidiogênese quantitativa na fase saprobiôntica Borges, Priscilla Rodrigues Fungos entomopatogênicos Controle de Aedes aegypti Toxicidade de aditivos Diferentes tempos de formulação Entomopathogenic fungi Control of Aedes aegypti Toxicity of additives Different formulation times CIENCIAS BIOLOGICAS::PARASITOLOGIA |
title_short |
Toxicidade de aditivos e de formulados de Metarhizium anisopliae s.I. IP46 em adultos de Aedes aegypti e conidiogênese quantitativa na fase saprobiôntica |
title_full |
Toxicidade de aditivos e de formulados de Metarhizium anisopliae s.I. IP46 em adultos de Aedes aegypti e conidiogênese quantitativa na fase saprobiôntica |
title_fullStr |
Toxicidade de aditivos e de formulados de Metarhizium anisopliae s.I. IP46 em adultos de Aedes aegypti e conidiogênese quantitativa na fase saprobiôntica |
title_full_unstemmed |
Toxicidade de aditivos e de formulados de Metarhizium anisopliae s.I. IP46 em adultos de Aedes aegypti e conidiogênese quantitativa na fase saprobiôntica |
title_sort |
Toxicidade de aditivos e de formulados de Metarhizium anisopliae s.I. IP46 em adultos de Aedes aegypti e conidiogênese quantitativa na fase saprobiôntica |
author |
Borges, Priscilla Rodrigues |
author_facet |
Borges, Priscilla Rodrigues |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Luz, Wolf Christian |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1104009511235835 |
dc.contributor.advisor-co1.fl_str_mv |
Fernandes, Éverton Kot Kamp |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/2135541732341157 |
dc.contributor.referee1.fl_str_mv |
Luz, Wolf Christian |
dc.contributor.referee2.fl_str_mv |
Santos, Solange Xavier dos |
dc.contributor.referee3.fl_str_mv |
Rocha, Luiz Fernando Nunes |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8824033277119685 |
dc.contributor.author.fl_str_mv |
Borges, Priscilla Rodrigues |
contributor_str_mv |
Luz, Wolf Christian Fernandes, Éverton Kot Kamp Luz, Wolf Christian Santos, Solange Xavier dos Rocha, Luiz Fernando Nunes |
dc.subject.por.fl_str_mv |
Fungos entomopatogênicos Controle de Aedes aegypti Toxicidade de aditivos Diferentes tempos de formulação |
topic |
Fungos entomopatogênicos Controle de Aedes aegypti Toxicidade de aditivos Diferentes tempos de formulação Entomopathogenic fungi Control of Aedes aegypti Toxicity of additives Different formulation times CIENCIAS BIOLOGICAS::PARASITOLOGIA |
dc.subject.eng.fl_str_mv |
Entomopathogenic fungi Control of Aedes aegypti Toxicity of additives Different formulation times |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::PARASITOLOGIA |
description |
Aedes aegypti is both the dengue and urban yellow fever vector in Brazil. Elimination of this mosquito is a strategy taken for the control of dengue. Entomopathogenic fungi such as Metarhizium anisopliae exhibit ovicidal, larvicidal and adulticidal activity on A. aegypti, being therefore of great interest for the integrated control of such mosquito. Water based, oil based, water/oil/powder based fungal formulations may help such fungi overtake environmental adverse effects as well as magnify their entomopathogenic action. Nevertheless, little is known about the effect of additives on A. aegypti adults. This study reports the effect of additives such as diatomaceous earth and oils (both vegetal and mineral), with or without the presence of M. anisopliae conidia IP 46 on A. aegypti adult mosquitoes, the effect of exposure time on accumulated mortality of such mosquitoes as well as the effects of formulations on conidiogenesis on dead adult mosquitoes killed by infection. Graxol ® vegetable oil was tested in two concentrations (0.25 and 0.05 µl/cm 2 ), as well as Naturol ® mineral oil (0,25 µl/cm 2 ) and KeepDry ® diatomaceous earth (0,6 mg/cm 2 ). Such additives were analyzed both separately and in combination (KeepDry ® + Graxol ® ; KeepDry ® + Naturol ® ). Subsequently, oily formulations or combinations of IP 46 at 3.3 x 10 6 conidia/cm 2 + KeepDry ® + oils (Graxol ® or Naturol ® ) were tested. Additives and formulations were applied inside plastic cups. Ten A. aegypti adults were placed in the cup and kept at 25°C, relative humidity of 75% and 12 hours of photophasis with permanent access to a 10% sugar solution. In order to assess the effect of the time of exposure, mosquitoes were exposed to IP 46 conidia (3.3 x 10 6 conidia/cm 2 ) formulations on diatomaceous earth and mineral oil for 0.016 hours (60s), 12 hours, 48 hours, 72 hours and 120 hours. A non-treated control group was kept at the same conditions. Mortality was daily verified for 15 days. Mosquitoes killed by fungal infection were incubated for 10 days in a humid chamber with relative humidity close to saturation (≥ 98%) and then suspended in Tween 80 ® (0,1%) for conidia quantification in Neubauer chamber. Results showed that the additives exert toxicity on A. aegypti adults as well as effect on mortality (F 6 , 35 = 31,9, P = 0,0001). IP 46 conidia combined to pure KeepDry ® or on oil associations took shorter to kill 50% (TL 50 ) of mosquitoes and the IP 46 + KD + N combination took less time to kill 90% (TL 90 ), namely 7.5 days. There was no relevant difference between time of exposure on accumulated mortality of adults exposed to combined and non-combined IP 46conidia. The growth of mycelia on dead adults started within 2 days of humid chamber incubation. New IP 46 conidia where observed from the 3 rd day on, regardless conidia concentration and combination type to which mosquitoes had previously been exposed to. The effect of the combination on quantitative conidiogenesis on dead mosquitoes was significantly different (F 5,18 = 3.8; P = 0.02), a greater number of conidia on dead mosquitoes being found on mosquitoes who were exposed to the IP 46 + KeepDry ® combination. The IP 46 with KeepDry ® and Naturol ® combination proved to be the most recommended one for the control of A. aegypti adults using a mycoinsecticide. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-02-15 |
dc.date.accessioned.fl_str_mv |
2019-02-07T10:41:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BORGES, P. R. Toxicidade de aditivos e de formulados de Metarhizium anisopliae s.I. IP46 em adultos de Aedes aegypti e conidiogênese quantitativa na fase saprobiôntica. 2012. 51 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/9277 |
identifier_str_mv |
BORGES, P. R. Toxicidade de aditivos e de formulados de Metarhizium anisopliae s.I. IP46 em adultos de Aedes aegypti e conidiogênese quantitativa na fase saprobiôntica. 2012. 51 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2012. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/9277 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
6085308344741430434 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
-7769011444564556288 |
dc.relation.cnpq.fl_str_mv |
-4544576747271574306 |
dc.relation.sponsorship.fl_str_mv |
2075167498588264571 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
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UFG |
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UFG |
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Biblioteca Digital de Teses e Dissertações da UFG |
collection |
Biblioteca Digital de Teses e Dissertações da UFG |
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repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tesesdissertacoes.bc@ufg.br ||tesesdissertacoes.bc@ufg.br |
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