Hipertermia magnética in vivo com nanopartículas de MnFe2O4 no tratamento de tumores sólidos e subcutâneos de Sarcoma 180

• Ano de defesa: 2017
• Autor(a) principal: Rodrigues, Harley Fernandes
• Orientador(a): Bakuzis, Andris Figueiroa
• Banca de defesa: Bakuzis, Andris Figueiroa, Lacerda, Elisângela de Paula Silveira, Silva, Carlos Jacinto da, Reis, Rui Manuel Vieira, Pontes, Renato Borges
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Fisica (IF)
• Departamento: Instituto de Física - IF (RG)
• País: Brasil
• Palavras-chave em Português: Hipertermia magnética; Nanopartículas magnéticas; Sarcoma 180; Termografia por infravermelho; Dose térmica
• Palavras-chave em Inglês: Magnetic hyperthermia; Magnetic nanoparticles; Infrared thermography; Thermal dose
• Área do conhecimento CNPq: CIENCIAS EXATAS E DA TERRA::FISICA
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/7389

In this thesis a methodology of real-time monitoring of magnetic hyperthermia (HM) in vivo was developed in the murine tumor model Sarcoma 180 using infrared thermography technique. Magnetic nanoparticles (NPM) consisted of Mn ferrites capable of generating heat at low magnetic field amplitude at the 300 kHz frequency within the safety limit determined by Atkinson. It has been shown that the apparent surface temperature value measured with the infrared camera underestimates the real skin temperature value of the mice if the camera objective does not form an angle 0 ° with the normal direction to the animal's skin in the region of interest on the tumor, with the error reaching more than 7.0 ° C (for 60 °). A new theoretical model to estimate the error in the temperature of curved surfaces was developed and proved valid even in the case where the surface temperature diverges significantly from the environment. Preclinical treatment results indicated a complete remission condition in animal that was submitted to 150 min of hyperthermia and other cases with partial remission, suggesting that biological response analyzes need to be done in a long time (> 60 days). Measurements of the intratumoral temperature monitored by three fiber-optic thermometers during the therapeutic procedure of HM with NPM indicated an inhomogeneous heat delivery within the tumor. Additionally, a new methodology for calculating the thermal dose (CEM43) evaluated at the surface, considering each pixel of the thermographic image as a thermometer in the tumor region, indicated that the value T10(t) of the temperature detected in vivo at the surface of the skin over subcutaneous tumors can report, with an error of the order of 5%, the mean intratumoral temperature value during the therapeutic procedure of HM.