Atividade antimicrobiana de óleos essenciais de Piper aduncum, Piper hispidinervum e Syzygium aromaticum e desenvolvimento de um antisséptico com princípio ativo natural

• Ano de defesa: 2010
• Autor(a) principal: Giviziez, Christiane Ricaldoni
• Orientador(a): Batista, Luis Roberto
• Banca de defesa: Silveira, Ivana Aparecida da, Nascimento, Andréia Peraro do
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: UNIVERSIDADE FEDERAL DE LAVRAS
• Programa de Pós-Graduação: DCA - Programa de Pós-graduação
• País: BRASIL
• Palavras-chave em Português: Atividade microbiana; Antissépticos; Antimicrobial activity; Antiseptic
• Área do conhecimento CNPq: CNPQ_NÃO_INFORMADO
• Link de acesso: https://repositorio.ufla.br/handle/1/2794

Essential oils have shown biological activity and serve as an alternative source of antimicrobial agents. Therefore, this study aimed to evaluate the biocide potential of essential oils of Piper hispidinervum, Piper aduncum and Syzygium aromaticum, as well to develop and to evaluate the profile of sensitivity of the antiseptic with active principle of essential oil of Syzygium aromaticum against pathogenic bacteria in vitro and in reducing the natural bacterial contamination of the hands. The obtainment of essential oil was performed by hydrodistillation technique using the modified Clevenger apparatus, and the identification and quantification of the constituents by analysis in GC / MS and GC-FID. The biological activity of essential oils on pathogenic bacteria was determined by Agar Perforating technique. For the inhibitory effect of filamentous fungi, it was used the disk diffusion test. To check the sensitivity to the antiseptic developed with 6.3% of essential oil of S. aromaticum, it was used in vitro stage, where it was detected the antimicrobial action on the bacteria Aeromonas hydrophila, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella typhi and Staphylococcus aureus. This also happened in the hand test, which was applied antiseptic formulated with essential oil of S. aromaticum on naturally contaminated hands. In chromatographic analyzes, the major constituents found in essential oil of Piper aduncum were (E)-nerolidol (19.30%) and trans-sabinene hydrate (18.79%), in the oil of Piper hispidinervum were safrole (73.44%) and p-mint-2,4(8)-diene (18.78%) and in the Syzygium aromaticum oil, eugenol (89.08%). The result of Minimum Inhibitory Concentration showed that all tested microorganisms showed sensibility to the oil of S. aromaticum. The oil of P. hispidinervum was more efficient for the fungi and the essential oil of P. aduncum showed the lower antimicrobial activity. In the data obtained in vitro and in antisepsis of the hands, the antiseptic with active principle of essential oil of S. aromaticum was effective against the microorganisms in different evaluated time periods.