Resposta olfativa de Telenomus podisi (Hymenoptera: Platygastridae) para voláteis de plantas induzidos por herbivoria e oviposição de Euschistus heros (Hemiptera: Pentatomidae)

• Ano de defesa: 2015
• Autor(a) principal: Dias, Aline Moreira
• Orientador(a): Piaggio, Martín Francisco Pareja
• Banca de defesa: Sampaio, Marcus Vinicius, Silveira, Luís Cláudio Paterno
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: UNIVERSIDADE FEDERAL DE LAVRAS
• Programa de Pós-Graduação: DEN - Departamento de Entomologia
• País: BRASIL
• Palavras-chave em Português: Parasitoide de ovos; Percevejo marrom; Voláteis de plantas; Egg parasitoid; Sting bug; Plant volatile
• Área do conhecimento CNPq: CNPQ_NÃO_INFORMADO
• Link de acesso: https://repositorio.ufla.br/handle/1/9605

Many chemical and morphological characteristics of plants influence insect foraging. Plants that suffer damage caused by herbivory and oviposition release different volatile compounds that attract parasitoids. This interaction is widely studied and used to enhance the biological control of pests in crops. In this study, we aimed at evaluating the olfactory response of the Telenomus podisi (Hymenoptera: Platygastridae) egg parasitoid to volatiles induced by Euschistus heros (Hemiptera, Pentatomidae) in three different plant species: maize (Zea mays), sunflower (Helianthus annuus) and pigeon pea (Cajanus cajan), as well as characterizing the chemical profile of these plants. In order to achieve this, we evaluated the response of parasitoid T. podisi to volatiles of each plant species, damaged or not by E. heros, later characterizing the profile of the volatiles of sunflower, pigeon pea and maize plants submitted to stinkbug attack. Each plant was treated with three E. heros virgin females (for herbivory) and four mated females (for oviposition), left for 72 hours to do damage. We tested the following combinations in Y-tube olfactometer bioassays: plants without damage against air, damaged plants against air, damaged plants against plants without damage and plants with herbivory damage against plant with herbivory + oviposition damage. The volatile profiles of the plants was identified by means of headspace dynamic collection and analysis in gas chromatography coupled to mass spectrometer. There was no difference in the responses of the parasitoid T. podisi to the combination of plants without damage against air and plants with herbivory damage against plant with herbivory + oviposition damage in any of the three plant species. In the treatment of damaged plants against air, there was a higher number of parasitoid females preferring the odor of maize plants with herbivory damage, and higher residence time in the olfactometer arm with the pigeon pea odor and herbivory damage. When contrasting damaged plants to plants without damage, the parasitoid showed higher number of initial choices for the odor of sunflower plants with herbivory damage, maize plants with herbivory and herbivory + oviposition damage, and greater residence time on pigeon pea plants with herbivory damage. The chemical profile of the plants was identified and showed qualitative difference between the plant compounds with and without damage. We can conclude that sunflower, maize and pigeon pea plants, when damaged by E. heros, release volatiles that attract the parasitoid T. podisi.