Óleos essenciais de Lippia sidoides Cham., Alomia fastigiata (Gardner) Benth, Ocotea odorifera (Vell.) Rohwer, Mikania glauca Mart. e Cordia verbenacea D.C.: identificação e quantificação química, caracterização das estruturas secretoras, atividades antioxidante e antibacteriana

• Ano de defesa: 2010
• Autor(a) principal: Guimarães, Luiz Gustavo de Lima
• Orientador(a): Cardoso, Maria das Graças
• Banca de defesa: Castro, Evaristo Mauro de, Lucas, Esther Maria Ferreira, Nelson, David Lee, Alves, Péricles Barreto
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: UNIVERSIDADE FEDERAL DE LAVRAS
• Programa de Pós-Graduação: DQI - Programa de Pós-graduação
• País: BRASIL
• Palavras-chave em Português: Metabólitos secundários; Ensaios antioxidantes; Atividade biológica; Estruturas secretoras; Secondary metabolites; Antioxidant assays; Biological activity; Secretory structures
• Área do conhecimento CNPq: CNPQ_NÃO_INFORMADO
• Link de acesso: https://repositorio.ufla.br/handle/1/2182

Because of the losses to the food industry generated by the oxidation of fats and oils, the damage caused to various crops by pathogens, and damage to the health of consumers caused by synthetic compounds used as antioxidants and agrochemicals, the identification and quantification of the essential oils from Lippia sidoides Cham., Alomia fastigiata (Gardner) Benth, Ocotea odorifera (Vell.) Rohwer, Mikania glauca Mart.and Cordia verbenacea (Verbenaceae) were undertaken. Their antioxidant and antibacterial activities were accessed, and the secretory structures for the essential oils were examined. The essential oils were extracted by steam distillation using a modified Clevenger apparatus, and were subsequently analyzed by GC-MS and GC-FID. The major constituent of the essential oil of Cordia verbenacea was isolated by column chromatography and subjected to analysis by one- and two-dimensional 1H and 13C NMR experiments. The antioxidant activity was determined by oxidation of the β-carotene/linoleic acid system, by reaction with reactive species of thiobarbituric acid (TBARS), and by the reduction of diphenylpicrylhydrazyl (DPPH) free radical. Biological tests for antibacterial activity against the Clavibacter michiganensis subsp. michiganensis, Xanthomonas vesicatoria, and Pseudomonas syringae pv. tomato phytobacteria were performed using the method of diffusion in an agar cavity. The characterization of secretory structures was accomplished by scanning electron microscopy (SEM) and by histochemical tests with Nadi and Sudan III reagents. Analysis of the essential oils by GC-MS demonstrated the presence of several terpene compounds (monoterpenes and sesquiterpenes) and some phenylpropanoids. The analysis by 1H and 13C NMR spectroscopy revealed the presence of an isomer of α-bisabolol, which was designated as isobisabolol, as the major constituent of the essential oil of C.verbenacea. The essential oils of the species used in this study and some of the major constituents presented relevant antioxidant and antibacterial activities. The highest activities in both biological assays were presented by the essential oil from L. sidoides and by its major constituent carvacrol. The characterization of the secretory structures for the essential oils demonstrated the presence of trichomem, secretory canals, and oleifera cells, which varied according to the species of plant.