Utilização de gonadotropina coriônica eqüina e/ou extrato bruto de hipófise de carpa na indução da reprodução de curimba (Prochilodus lineatus)

• Ano de defesa: 2006
• Autor(a) principal: Pereira, Gilmara Junqueira Machado
• Orientador(a): Murgas, Luis David Solis
• Banca de defesa: Pimenta, Carlos José, Ribeiro Filho, Oswaldo Pinto, Zangerônimo, Márcio Gilberto, Logato, Priscila Vieira Rosa
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: UNIVERSIDADE FEDERAL DE LAVRAS
• Programa de Pós-Graduação: DMV - Programa de Pós-graduação; Programa de Pós-Graduação em Ciências Veterinárias
• País: BRASIL
• Palavras-chave em Português: Reproducao animal; Reprodução induzida; Desova; Sêmen; Peixe; Induction of reproduction; Spawning; Sperm; Fishes
• Área do conhecimento CNPq: CNPQ_NÃO_INFORMADO
• Link de acesso: https://repositorio.ufla.br/handle/1/2368

The present study evaluated the effect of the utilization of eCG (equine chorionic gonadotropin) and of the CPE (carp pituitary extract) combined or not, used in the induction of reproduction of males and females of curimba (Prochilodus lineatus). Four different hormonal treatments have been tested, with two doses with interval of 12 h (A: 0,5 CPE mg/kg and 5,0 CPE mg/kg; B: 0,5 CPE mg/kg and 5,0 CPE mg/kg + 500 eCG IU/kg; C: 0,5 CPE mg/kg and 500 eCG IU/kg; D: 500 eCG IU/kg and 500 eCG IU/kg). In the females only the three first treatments have been tested. In the males the evaluated seminal parameters were, volume of the semen, sperm motility, motility duration, spermatozoa concentration and sperm morphology. In the females, samples of oocytes had been collected, five and eight hours after second hormonal application, to analyze the position of the germinative vesicle (nucleus) and the oocytes diameter. Another evaluated parameter was the weight of the spawning on the induced females. For the females, it did not have significant difference on the weight of the spawning of the induced females for the treatments A and the B. The induced females for treatment C had not spawned. In the evaluation of the oocytes diameter, it did not have significant difference on the treatments five hours after the second hormonal application, whereas, eight hours after the second hormonal application treatments B and C had been significant different (P< 0,05) to the treatment A. In the evaluation of the positioning of the germinative vesicle, the treatments A and B had been significant different of treatment C, five hours as much as eight hours after the second hormonal application. For the males, the results had not presented significant difference between the used hormonal protocols, with regard to the volume of the semen, sperm motility, motility duration and spermatozoa concentration (P<0,05). The treatment which used two doses of 500 IU/kg of eCG, was significant different of the others treatment, presenting a lower number of spermatozoa with abnormality. It can be concluded that eCG did not reveal efficient when not combined with the CPE in the second hormonal application, in the induction of the spawning of females of curimba, however for the males, eCG (500 and 500 IU/kg) can substitute the CPE (0,5 and 5,0 mg/kg) without modifying the evaluated seminal characteristics.