Emprego da Microscopia Eletroquímica de Varredura SECM na investigação de processos interfaciais visando o desenvolvimento de sensores químicos
| Ano de defesa: | 2017 |
|---|---|
| Autor(a) principal: |
Macena, Cleidivan Silva
 |
| Orientador(a): | |
| Banca de defesa: | |
| Tipo de documento: | Dissertação |
| Tipo de acesso: | Acesso aberto |
| Idioma: | por |
| Instituição de defesa: |
Universidade Federal do Maranhão
|
| Programa de Pós-Graduação: |
PROGRAMA DE PÓS-GRADUAÇÃO EM QUÍMICA/CCET
|
| Departamento: |
DEPARTAMENTO DE TECNOLOGIA QUÍMICA/CCET
|
| País: |
Brasil
|
| Palavras-chave em Português: | |
| Palavras-chave em Inglês: | |
| Área do conhecimento CNPq: | |
| Link de acesso: | http://tedebc.ufma.br:8080/jspui/handle/tede/1733 |
Resumo: | The present work demonstrated the potential of Scanning Electrochemical Microscopy (SECM) in the understanding of enzymatic systems using the enzymes glucose oxidase (GOx) and the peroxidase of strong root (HRP) as recognition model. In addition, it evaluated different platforms, such as: Graphene (Gr) and Carbon Nanotubes (CNTs) in the response of enzymatic systems through approximation curves and images. In this sense, the reactivity of the GOx and HRP enzymes was evaluated in the generator / collector mode on an insulating platform. In addition, the HRP was studied comparatively on graphene platform Gr, CNTs and on conductive surface. The use of the SECM technique in the investigation of these systems showed its applicability in the investigation of reactions catalyzed by enzymes and also showed that CNTs were the best platform for immobilization of HRP. In addition, it showed the application of SECM in enzymatic systems for the selective determination of compounds isomers: catechol and hydroquinone. |
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DAMOS, Flávio Santos846.255.703-87032.267.063-22http://lattes.cnpq.br/4159386811870319Macena, Cleidivan Silva2017-07-07T18:52:22Z2017-02-03MACENA, Cleidivan Silva. Emprego da Microscopia Eletroquímica de Varredura SECM na investigação de processos interfaciais visando o desenvolvimento de sensores químicos. 2017. 111 f. Dissertação (Mestrado em Química) - Universidade Federal do Maranhão, São Luís, 2017.http://tedebc.ufma.br:8080/jspui/handle/tede/1733The present work demonstrated the potential of Scanning Electrochemical Microscopy (SECM) in the understanding of enzymatic systems using the enzymes glucose oxidase (GOx) and the peroxidase of strong root (HRP) as recognition model. In addition, it evaluated different platforms, such as: Graphene (Gr) and Carbon Nanotubes (CNTs) in the response of enzymatic systems through approximation curves and images. In this sense, the reactivity of the GOx and HRP enzymes was evaluated in the generator / collector mode on an insulating platform. In addition, the HRP was studied comparatively on graphene platform Gr, CNTs and on conductive surface. The use of the SECM technique in the investigation of these systems showed its applicability in the investigation of reactions catalyzed by enzymes and also showed that CNTs were the best platform for immobilization of HRP. In addition, it showed the application of SECM in enzymatic systems for the selective determination of compounds isomers: catechol and hydroquinone.O presente trabalho demostrou a potencialidade da Microscopia Eletroquímica de Varredura (Scanning Electrochemical Microscopy - SECM) no entendimento de sistemas enzimáticos usando as enzimas glicose oxidase (GOx) e a peroxidase de raiz forte (HRP) como modelo de reconhecimento. Ademais, avaliou diferentes plataformas, como: Grafeno (Gr) e Nanotubos de Carbono (CNTs) na resposta de sistemas enzimáticos através de curvas de aproximação e imagens. Neste sentido, a reatividade das enzimas GOx e HRP foi avaliada no modo gerador/coletor sobre plataforma isolante. Adicionalmente, a HRP foi estudada comparativamente sobre plataforma de grafeno Gr, CNTs e sobre superfície condutora. O uso da técnica SECM na investigação desses sistemas mostrou sua aplicabilidade na investigação de reações catalisadas por enzimas e mostrou ainda que os CNTs fora a melhor plataforma para imobilização da HRP. Além disso, mostrou a aplicação da SECM em sistemas enzimáticos para a determinação seletiva de compostos isômeros: catecol e hidroquinona.Submitted by Rosivalda Pereira (mrs.pereira@ufma.br) on 2017-07-07T18:52:22Z No. of bitstreams: 1 CleidivanMacena.pdf: 2189360 bytes, checksum: 599a50d965fad9ff9559783fe4574feb (MD5)Made available in DSpace on 2017-07-07T18:52:22Z (GMT). No. of bitstreams: 1 CleidivanMacena.pdf: 2189360 bytes, checksum: 599a50d965fad9ff9559783fe4574feb (MD5) Previous issue date: 2017-02-03Fundação de Amparo à Pesquisa e ao Desenvolvimento Científico e Tecnológico do Maranhão (FAPEMA)application/pdfporUniversidade Federal do MaranhãoPROGRAMA DE PÓS-GRADUAÇÃO EM QUÍMICA/CCETUFMABrasilDEPARTAMENTO DE TECNOLOGIA QUÍMICA/CCETMicroscopia Eletroquímica de VarreduraPeroxidase de raiz forteGlicose oxidaseGrafenoNanotubos de carbonoScanning Electrochemical MicroscopyGlucose oxidasePeroxidase of strong rootGrapheneCarbon NanotubesQuímica AnalíticaEmprego da Microscopia Eletroquímica de Varredura SECM na investigação de processos interfaciais visando o desenvolvimento de sensores químicosScanning Electrochemical Microscopy in the investigation of interfacial processes aimed at development of chemical sensorsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFMAinstname:Universidade Federal do Maranhão (UFMA)instacron:UFMAORIGINALCleidivanMacena.pdfCleidivanMacena.pdfapplication/pdf2189360http://tedebc.ufma.br:8080/bitstream/tede/1733/2/CleidivanMacena.pdf599a50d965fad9ff9559783fe4574febMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82255http://tedebc.ufma.br:8080/bitstream/tede/1733/1/license.txt97eeade1fce43278e63fe063657f8083MD51tede/17332017-12-07 13:57:28.859oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttps://tedebc.ufma.br/jspui/PUBhttp://tedebc.ufma.br:8080/oai/requestrepositorio@ufma.br||repositorio@ufma.bropendoar:21312017-12-07T16:57:28Biblioteca Digital de Teses e Dissertações da UFMA - Universidade Federal do Maranhão (UFMA)false |
| dc.title.por.fl_str_mv |
Emprego da Microscopia Eletroquímica de Varredura SECM na investigação de processos interfaciais visando o desenvolvimento de sensores químicos |
| dc.title.alternative.por.fl_str_mv |
Scanning Electrochemical Microscopy in the investigation of interfacial processes aimed at development of chemical sensors |
| title |
Emprego da Microscopia Eletroquímica de Varredura SECM na investigação de processos interfaciais visando o desenvolvimento de sensores químicos |
| spellingShingle |
Emprego da Microscopia Eletroquímica de Varredura SECM na investigação de processos interfaciais visando o desenvolvimento de sensores químicos Macena, Cleidivan Silva Microscopia Eletroquímica de Varredura Peroxidase de raiz forte Glicose oxidase Grafeno Nanotubos de carbono Scanning Electrochemical Microscopy Glucose oxidase Peroxidase of strong root Graphene Carbon Nanotubes Química Analítica |
| title_short |
Emprego da Microscopia Eletroquímica de Varredura SECM na investigação de processos interfaciais visando o desenvolvimento de sensores químicos |
| title_full |
Emprego da Microscopia Eletroquímica de Varredura SECM na investigação de processos interfaciais visando o desenvolvimento de sensores químicos |
| title_fullStr |
Emprego da Microscopia Eletroquímica de Varredura SECM na investigação de processos interfaciais visando o desenvolvimento de sensores químicos |
| title_full_unstemmed |
Emprego da Microscopia Eletroquímica de Varredura SECM na investigação de processos interfaciais visando o desenvolvimento de sensores químicos |
| title_sort |
Emprego da Microscopia Eletroquímica de Varredura SECM na investigação de processos interfaciais visando o desenvolvimento de sensores químicos |
| author |
Macena, Cleidivan Silva |
| author_facet |
Macena, Cleidivan Silva |
| author_role |
author |
| dc.contributor.advisor1.fl_str_mv |
DAMOS, Flávio Santos |
| dc.contributor.advisor1ID.fl_str_mv |
846.255.703-87 |
| dc.contributor.authorID.fl_str_mv |
032.267.063-22 |
| dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4159386811870319 |
| dc.contributor.author.fl_str_mv |
Macena, Cleidivan Silva |
| contributor_str_mv |
DAMOS, Flávio Santos |
| dc.subject.por.fl_str_mv |
Microscopia Eletroquímica de Varredura Peroxidase de raiz forte Glicose oxidase Grafeno Nanotubos de carbono |
| topic |
Microscopia Eletroquímica de Varredura Peroxidase de raiz forte Glicose oxidase Grafeno Nanotubos de carbono Scanning Electrochemical Microscopy Glucose oxidase Peroxidase of strong root Graphene Carbon Nanotubes Química Analítica |
| dc.subject.eng.fl_str_mv |
Scanning Electrochemical Microscopy Glucose oxidase Peroxidase of strong root Graphene Carbon Nanotubes |
| dc.subject.cnpq.fl_str_mv |
Química Analítica |
| description |
The present work demonstrated the potential of Scanning Electrochemical Microscopy (SECM) in the understanding of enzymatic systems using the enzymes glucose oxidase (GOx) and the peroxidase of strong root (HRP) as recognition model. In addition, it evaluated different platforms, such as: Graphene (Gr) and Carbon Nanotubes (CNTs) in the response of enzymatic systems through approximation curves and images. In this sense, the reactivity of the GOx and HRP enzymes was evaluated in the generator / collector mode on an insulating platform. In addition, the HRP was studied comparatively on graphene platform Gr, CNTs and on conductive surface. The use of the SECM technique in the investigation of these systems showed its applicability in the investigation of reactions catalyzed by enzymes and also showed that CNTs were the best platform for immobilization of HRP. In addition, it showed the application of SECM in enzymatic systems for the selective determination of compounds isomers: catechol and hydroquinone. |
| publishDate |
2017 |
| dc.date.accessioned.fl_str_mv |
2017-07-07T18:52:22Z |
| dc.date.issued.fl_str_mv |
2017-02-03 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
MACENA, Cleidivan Silva. Emprego da Microscopia Eletroquímica de Varredura SECM na investigação de processos interfaciais visando o desenvolvimento de sensores químicos. 2017. 111 f. Dissertação (Mestrado em Química) - Universidade Federal do Maranhão, São Luís, 2017. |
| dc.identifier.uri.fl_str_mv |
http://tedebc.ufma.br:8080/jspui/handle/tede/1733 |
| identifier_str_mv |
MACENA, Cleidivan Silva. Emprego da Microscopia Eletroquímica de Varredura SECM na investigação de processos interfaciais visando o desenvolvimento de sensores químicos. 2017. 111 f. Dissertação (Mestrado em Química) - Universidade Federal do Maranhão, São Luís, 2017. |
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por |
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por |
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Universidade Federal do Maranhão |
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PROGRAMA DE PÓS-GRADUAÇÃO EM QUÍMICA/CCET |
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UFMA |
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Brasil |
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DEPARTAMENTO DE TECNOLOGIA QUÍMICA/CCET |
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Universidade Federal do Maranhão |
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