Papel protetor das citocinas IL-9/IL-10 em lesões perriradiculares humanas

Detalhes bibliográficos
Ano de defesa: 2017
Autor(a) principal: Lucas Moreira Maia
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Minas Gerais
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Periodontite periapical
Interleucina-9
Quimiocinas
Citocinas
Link de acesso: https://hdl.handle.net/1843/ODON-AZWKZF
Resumo: This study aims to identify the gene expression of a new group of T lymphocytes, Th9 cells, characteristically responsible for producing IL-9 in the periradicular tissues of individuals with endodontic infections submitted to routine endodontic therapy, in the presence and absence of infection as well as the cytokines TNF-, IL-1. IL-9, INF- and IL-10 and CCL-2 / MCP-1 and CCR-6 chemokines in the periapical interstitial fluid of human root canal infections. Samples were collected immediately after cleaning and formatting procedures and 7 days later (after reduction of intracanal microbial load) to characterize the expression of these genes. Real-time polymerase chain reaction demonstrated significantly higher levels of IL-1, IL-9, INF-, TNF- and IL-10 markers at day 7 compared to day 0. In turn, the CCL-2 / MCP-1 and CCR-6 chemokines and IL- 17A cytokine showed no significant differences in mRNA expression between the 2 periods analyzed. In analyzing the clinical variation after endodontic therapy on periapical immune status, this study demonstrated that the cytokine and chemokinemediated proinflammatory response appears to be modulated in a IL-10 / IL-9 dependent manner.
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spelling 2019-08-14T14:13:10Z2025-09-09T00:42:40Z2019-08-14T14:13:10Z2017-07-31https://hdl.handle.net/1843/ODON-AZWKZFThis study aims to identify the gene expression of a new group of T lymphocytes, Th9 cells, characteristically responsible for producing IL-9 in the periradicular tissues of individuals with endodontic infections submitted to routine endodontic therapy, in the presence and absence of infection as well as the cytokines TNF-, IL-1. IL-9, INF- and IL-10 and CCL-2 / MCP-1 and CCR-6 chemokines in the periapical interstitial fluid of human root canal infections. Samples were collected immediately after cleaning and formatting procedures and 7 days later (after reduction of intracanal microbial load) to characterize the expression of these genes. Real-time polymerase chain reaction demonstrated significantly higher levels of IL-1, IL-9, INF-, TNF- and IL-10 markers at day 7 compared to day 0. In turn, the CCL-2 / MCP-1 and CCR-6 chemokines and IL- 17A cytokine showed no significant differences in mRNA expression between the 2 periods analyzed. In analyzing the clinical variation after endodontic therapy on periapical immune status, this study demonstrated that the cytokine and chemokinemediated proinflammatory response appears to be modulated in a IL-10 / IL-9 dependent manner.Universidade Federal de Minas GeraisCitocinaTH9Periodontite ApicalQuimiocinaInterleucina IL-9Periodontite periapicalInterleucina-9QuimiocinasCitocinasPapel protetor das citocinas IL-9/IL-10 em lesões perriradiculares humanasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisLucas Moreira Maiainfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGAntonio Paulino Ribeiro SobrinhoEvandro Neves AbdoGil Moreira JúniorEste estudo tem como objeitvo Identificar a expressao genica de um novo grupo de linfocitos T, as celulas Th9, caracteristicamente responsavel por produzirem a IL-9 nos tecidos perirradiculares de individuos portadores de infeccoes endodonticas submetidos a terapia endodontica de rotina, na presenca e ausencia de infeccao assim como as citocinas TNF-, IL-1. IL-9, INF- e IL-10 e das quimiocinas CCL-2/MCP-1 e CCR-6 no fluido intersticial periapical de infeccoes de canais radiculares humanas. As amostras foram coletadas imediatamente apos os procedimentos de limpeza e formatacao e 7dias mais tarde (apos reducao da carga microbiana intracanal) para caracterizar a expressao destes genes. A reacao em cadeia da polimerase em tempo real demonstrou niveis significativamente maiores de marcadores de IL-1, IL-9, INF-, TNF- e IL-10 no dia 7 quando comparado com ao dia 0. Por sua vez, as quimiocinas CCL-2/MCP-1 e CCR-6 e a citocina IL-17A nao apresentaram diferencas significativas na expressao de mRNA entre os 2 periodos analisados. Ao analisar a variacao clinica pos terapia endodontica sobre a condicao imune periapical, este estudo demonstrou que a resposta pro-inflamatoria mediada por citocinas e quimiocinas parece ser modulada de forma IL-10/IL-9 dependente.UFMGORIGINALpapel_protetor_das_citocinas_il_9il_10_em_leso_es_perriradiculares__humanas.pdfapplication/pdf528540https://repositorio.ufmg.br//bitstreams/63de50b3-7a27-4b0d-aaa4-ec8b5c46fa11/download87b239e6d39f59fc7b6e3f8204004249MD51trueAnonymousREADTEXTpapel_protetor_das_citocinas_il_9il_10_em_leso_es_perriradiculares__humanas.pdf.txttext/plain55472https://repositorio.ufmg.br//bitstreams/fa8eea59-f8c2-4507-bf24-4b3d70c93f64/downloadce6a41d16c229dd691c837dbee2569f0MD52falseAnonymousREAD1843/ODON-AZWKZF2025-09-08 21:42:40.029open.accessoai:repositorio.ufmg.br:1843/ODON-AZWKZFhttps://repositorio.ufmg.br/Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2025-09-09T00:42:40Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Papel protetor das citocinas IL-9/IL-10 em lesões perriradiculares humanas
title Papel protetor das citocinas IL-9/IL-10 em lesões perriradiculares humanas
spellingShingle Papel protetor das citocinas IL-9/IL-10 em lesões perriradiculares humanas
Lucas Moreira Maia
Periodontite periapical
Interleucina-9
Quimiocinas
Citocinas
Citocina
TH9
Periodontite Apical
Quimiocina
Interleucina IL-9
title_short Papel protetor das citocinas IL-9/IL-10 em lesões perriradiculares humanas
title_full Papel protetor das citocinas IL-9/IL-10 em lesões perriradiculares humanas
title_fullStr Papel protetor das citocinas IL-9/IL-10 em lesões perriradiculares humanas
title_full_unstemmed Papel protetor das citocinas IL-9/IL-10 em lesões perriradiculares humanas
title_sort Papel protetor das citocinas IL-9/IL-10 em lesões perriradiculares humanas
author Lucas Moreira Maia
author_facet Lucas Moreira Maia
author_role author
dc.contributor.author.fl_str_mv Lucas Moreira Maia
dc.subject.por.fl_str_mv Periodontite periapical
Interleucina-9
Quimiocinas
Citocinas
topic Periodontite periapical
Interleucina-9
Quimiocinas
Citocinas
Citocina
TH9
Periodontite Apical
Quimiocina
Interleucina IL-9
dc.subject.other.none.fl_str_mv Citocina
TH9
Periodontite Apical
Quimiocina
Interleucina IL-9
description This study aims to identify the gene expression of a new group of T lymphocytes, Th9 cells, characteristically responsible for producing IL-9 in the periradicular tissues of individuals with endodontic infections submitted to routine endodontic therapy, in the presence and absence of infection as well as the cytokines TNF-, IL-1. IL-9, INF- and IL-10 and CCL-2 / MCP-1 and CCR-6 chemokines in the periapical interstitial fluid of human root canal infections. Samples were collected immediately after cleaning and formatting procedures and 7 days later (after reduction of intracanal microbial load) to characterize the expression of these genes. Real-time polymerase chain reaction demonstrated significantly higher levels of IL-1, IL-9, INF-, TNF- and IL-10 markers at day 7 compared to day 0. In turn, the CCL-2 / MCP-1 and CCR-6 chemokines and IL- 17A cytokine showed no significant differences in mRNA expression between the 2 periods analyzed. In analyzing the clinical variation after endodontic therapy on periapical immune status, this study demonstrated that the cytokine and chemokinemediated proinflammatory response appears to be modulated in a IL-10 / IL-9 dependent manner.
publishDate 2017
dc.date.issued.fl_str_mv 2017-07-31
dc.date.accessioned.fl_str_mv 2019-08-14T14:13:10Z
2025-09-09T00:42:40Z
dc.date.available.fl_str_mv 2019-08-14T14:13:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1843/ODON-AZWKZF
url https://hdl.handle.net/1843/ODON-AZWKZF
dc.language.iso.fl_str_mv por
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
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institution UFMG
reponame_str Repositório Institucional da UFMG
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