Isolamento, tipificação e genotipagem de Brucella abortus isoladas de bovinos no Brasil

Detalhes bibliográficos
Ano de defesa: 2009
Autor(a) principal: Silvia Minharro Barbosa
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Minas Gerais
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Bovino Doenças
Brucelose em bovino
Brucella abortus
B abortus
tipificação
diagnóstico
Link de acesso: https://hdl.handle.net/1843/LGPD-7SUNFX
Resumo: The aims of the present study were (i) to evaluate isolation methods for Brucella spp. and (ii) to biotype and genotype B. abortus isolates from cattle in Brazil, in order to support the Programa Nacional de Controle e Erradicação de Brucelose e Tuberculose (National Program on the Control and Erradication of Brucellosis and Tuberculosis) (PCNEBT). Enrichment using tryptose broth supplemented with Farrel antimicrobials increased the isolation rate of Brucella spp. 51.36% in clinical material from slaughterhouses (74/187) versus direct plating (36/187). Most of isolates were obtained after 7-day enrichment. From 137 Brazilian B. abortus isolates (from 1977 to 2008) biovars 1, 2, and 3 were confirmed and biovars 4 and 6 were identified for the first time. All biovar 3 isolates were classified as subgroup 3b by AMOS-ERY PCR. Three biovar 3 isolates from State of Pará amplified in AMOS PCR and could become an epidemiological marker. MLVA16 panel 1 identified two cluster (I and II): one that mainly clusters around genotype 40 strains and another that clusters around genotype 28. MLVA16 panels 2A and 2B showed high diversity among the 137 studied strains, identifying 89 genotypes. MLVA16 helped knowing the distribution of genotypes and will help to build a Brazilian B. abortus genotype database
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spelling Isolamento, tipificação e genotipagem de Brucella abortus isoladas de bovinos no BrasilBovino DoençasBrucelose em bovinoBrucella abortusB abortustipificaçãodiagnósticoThe aims of the present study were (i) to evaluate isolation methods for Brucella spp. and (ii) to biotype and genotype B. abortus isolates from cattle in Brazil, in order to support the Programa Nacional de Controle e Erradicação de Brucelose e Tuberculose (National Program on the Control and Erradication of Brucellosis and Tuberculosis) (PCNEBT). Enrichment using tryptose broth supplemented with Farrel antimicrobials increased the isolation rate of Brucella spp. 51.36% in clinical material from slaughterhouses (74/187) versus direct plating (36/187). Most of isolates were obtained after 7-day enrichment. From 137 Brazilian B. abortus isolates (from 1977 to 2008) biovars 1, 2, and 3 were confirmed and biovars 4 and 6 were identified for the first time. All biovar 3 isolates were classified as subgroup 3b by AMOS-ERY PCR. Three biovar 3 isolates from State of Pará amplified in AMOS PCR and could become an epidemiological marker. MLVA16 panel 1 identified two cluster (I and II): one that mainly clusters around genotype 40 strains and another that clusters around genotype 28. MLVA16 panels 2A and 2B showed high diversity among the 137 studied strains, identifying 89 genotypes. MLVA16 helped knowing the distribution of genotypes and will help to build a Brazilian B. abortus genotype databaseUniversidade Federal de Minas Gerais2019-08-11T10:06:47Z2025-09-09T01:29:07Z2019-08-11T10:06:47Z2009-02-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttps://hdl.handle.net/1843/LGPD-7SUNFXSilvia Minharro Barbosainfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG2025-09-09T18:55:48Zoai:repositorio.ufmg.br:1843/LGPD-7SUNFXRepositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2025-09-09T18:55:48Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Isolamento, tipificação e genotipagem de Brucella abortus isoladas de bovinos no Brasil
title Isolamento, tipificação e genotipagem de Brucella abortus isoladas de bovinos no Brasil
spellingShingle Isolamento, tipificação e genotipagem de Brucella abortus isoladas de bovinos no Brasil
Silvia Minharro Barbosa
Bovino Doenças
Brucelose em bovino
Brucella abortus
B abortus
tipificação
diagnóstico
title_short Isolamento, tipificação e genotipagem de Brucella abortus isoladas de bovinos no Brasil
title_full Isolamento, tipificação e genotipagem de Brucella abortus isoladas de bovinos no Brasil
title_fullStr Isolamento, tipificação e genotipagem de Brucella abortus isoladas de bovinos no Brasil
title_full_unstemmed Isolamento, tipificação e genotipagem de Brucella abortus isoladas de bovinos no Brasil
title_sort Isolamento, tipificação e genotipagem de Brucella abortus isoladas de bovinos no Brasil
author Silvia Minharro Barbosa
author_facet Silvia Minharro Barbosa
author_role author
dc.contributor.author.fl_str_mv Silvia Minharro Barbosa
dc.subject.por.fl_str_mv Bovino Doenças
Brucelose em bovino
Brucella abortus
B abortus
tipificação
diagnóstico
topic Bovino Doenças
Brucelose em bovino
Brucella abortus
B abortus
tipificação
diagnóstico
description The aims of the present study were (i) to evaluate isolation methods for Brucella spp. and (ii) to biotype and genotype B. abortus isolates from cattle in Brazil, in order to support the Programa Nacional de Controle e Erradicação de Brucelose e Tuberculose (National Program on the Control and Erradication of Brucellosis and Tuberculosis) (PCNEBT). Enrichment using tryptose broth supplemented with Farrel antimicrobials increased the isolation rate of Brucella spp. 51.36% in clinical material from slaughterhouses (74/187) versus direct plating (36/187). Most of isolates were obtained after 7-day enrichment. From 137 Brazilian B. abortus isolates (from 1977 to 2008) biovars 1, 2, and 3 were confirmed and biovars 4 and 6 were identified for the first time. All biovar 3 isolates were classified as subgroup 3b by AMOS-ERY PCR. Three biovar 3 isolates from State of Pará amplified in AMOS PCR and could become an epidemiological marker. MLVA16 panel 1 identified two cluster (I and II): one that mainly clusters around genotype 40 strains and another that clusters around genotype 28. MLVA16 panels 2A and 2B showed high diversity among the 137 studied strains, identifying 89 genotypes. MLVA16 helped knowing the distribution of genotypes and will help to build a Brazilian B. abortus genotype database
publishDate 2009
dc.date.none.fl_str_mv 2009-02-17
2019-08-11T10:06:47Z
2019-08-11T10:06:47Z
2025-09-09T01:29:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1843/LGPD-7SUNFX
url https://hdl.handle.net/1843/LGPD-7SUNFX
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
repository.name.fl_str_mv Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv repositorio@ufmg.br
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