Avaliação de vacinas contra Clostridium perfringens tipos C e D

Detalhes bibliográficos
Ano de defesa: 1997
Autor(a) principal: Edisio Oliveira de Azevedo
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Minas Gerais
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Clostridiose Vacinas
Link de acesso: https://hdl.handle.net/1843/BUOS-8PQKNM
Resumo: Seven commercial vaccines against clostridiosis. which had in their composition type C and/or D Closmdzum pemingens toxoids. have been evaluated for sterility, residual toxicity and potency. Six vaccines were produced in Brazil and one was imported, a bivalent toxoid standard of C. perjiingens type C and D has been used as a test control. All tested vaccines were sterile when cultivated in bacteria and fungi growth media. and were innocuous when administrated to mice via intraperitoneal route. ln relation to the potency, evaluated by mice semm neutralization tests using immunized rabbits sera pooled the imported vaccine and the standard toxoid have presented higher serum antibodies titres than the required minimum level of 10 and 5 IU/mL for beta and epsilon toxins produced from type C and D C. peyiirigens, respectively. The brazilian made vaccines were ineffective in stimulating serum antibodies levels specetie to beta and epilon toxins, according to the recommended test level for quality control. Four out of six brazilian made toxoids were also tested in goats but did not presefnt detectable antitoxins levels within the test level of L+ or L+/W used for beta and epsilon toxins. respectively. The toxins utilized have been produced cultivating C. pemingens within in dialisys membrane.
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spelling 2019-08-10T05:17:58Z2025-09-09T00:09:22Z2019-08-10T05:17:58Z1997-02-17https://hdl.handle.net/1843/BUOS-8PQKNMSeven commercial vaccines against clostridiosis. which had in their composition type C and/or D Closmdzum pemingens toxoids. have been evaluated for sterility, residual toxicity and potency. Six vaccines were produced in Brazil and one was imported, a bivalent toxoid standard of C. perjiingens type C and D has been used as a test control. All tested vaccines were sterile when cultivated in bacteria and fungi growth media. and were innocuous when administrated to mice via intraperitoneal route. ln relation to the potency, evaluated by mice semm neutralization tests using immunized rabbits sera pooled the imported vaccine and the standard toxoid have presented higher serum antibodies titres than the required minimum level of 10 and 5 IU/mL for beta and epsilon toxins produced from type C and D C. peyiirigens, respectively. The brazilian made vaccines were ineffective in stimulating serum antibodies levels specetie to beta and epilon toxins, according to the recommended test level for quality control. Four out of six brazilian made toxoids were also tested in goats but did not presefnt detectable antitoxins levels within the test level of L+ or L+/W used for beta and epsilon toxins. respectively. The toxins utilized have been produced cultivating C. pemingens within in dialisys membrane.Universidade Federal de Minas GeraisToxinasEnterotoxemiaVacinasC perfringensClostridiose VacinasAvaliação de vacinas contra Clostridium perfringens tipos C e Dinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisEdisio Oliveira de Azevedoinfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGFrancisco Carlos Faria LobatoVera Lucia Viegas de AbreuPedro Lucio Lithg PereiraNelson Rodrigo da Silva MartinsSete vacinas comerciais contra clostridioses, que continham em sua composição toxóides de Clostridium perfringens tipos C e/ou D, foram avaliadas quanto à esterilidade, inocuidade c eficiência. Seis vacinas foram produzidas no Brasil e uma foi importada. Como controle dos testes, empregou-se um toxóide bivalente padrão de C. perfringens C e D. Todas as vacinas testadas foram estéreis quando semeadas em meios para pesquisa dc bactérias e fungos e mostram-se inócuas quando administradas por via intraperitoneal em camundongos. Quanto à eficiência, determinada pelo teste de soro-neutralização em camundongos a partir do `pool" de soros de coelhos imunizados, a vacina importada e o toxóide padrão, apresentaram títulos de anticorpos séricos superiores aos níveis mínimos exigidos de 10 e 5 UI/mL para as toxinas beta e épsilon, produzidas por C. perfringens tipos C e D, respectivamente. As vacinas produzidas no Brasil foram ineficientes em estimular níveis sorológicos de beta e épsilon antitoxinas compatíveis com os níveis de teste recomendados para controle destes produtos. Quatro dos seis toxóides de origem nacional, testados em caprinos, não apresentaram níveis de antitoxinas detectáveis com os níveis de testes de L+ ou L+/10 para beta e épsilon toxinas, respectivamente. As toxinas utilizadas para realização dos testes foram produzidas em membrana de diálise.UFMGORIGINALdisserta__o_de_mestrado_de_edisio_oliveira_de_azevedo.pdfapplication/pdf10943343https://repositorio.ufmg.br//bitstreams/cf7f3eb9-4764-4790-8e30-4a19edc02a73/download8bbabcc2ceb27d537b0d1e77935702dbMD51trueAnonymousREADTEXTdisserta__o_de_mestrado_de_edisio_oliveira_de_azevedo.pdf.txttext/plain50https://repositorio.ufmg.br//bitstreams/04d7309c-5e47-4d24-a833-4404969702c5/downloada0fa1f4054dc8804363acb0c624caf3aMD52falseAnonymousREAD1843/BUOS-8PQKNM2025-09-08 21:09:22.749open.accessoai:repositorio.ufmg.br:1843/BUOS-8PQKNMhttps://repositorio.ufmg.br/Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2025-09-09T00:09:22Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Avaliação de vacinas contra Clostridium perfringens tipos C e D
title Avaliação de vacinas contra Clostridium perfringens tipos C e D
spellingShingle Avaliação de vacinas contra Clostridium perfringens tipos C e D
Edisio Oliveira de Azevedo
Clostridiose Vacinas
Toxinas
Enterotoxemia
Vacinas
C perfringens
title_short Avaliação de vacinas contra Clostridium perfringens tipos C e D
title_full Avaliação de vacinas contra Clostridium perfringens tipos C e D
title_fullStr Avaliação de vacinas contra Clostridium perfringens tipos C e D
title_full_unstemmed Avaliação de vacinas contra Clostridium perfringens tipos C e D
title_sort Avaliação de vacinas contra Clostridium perfringens tipos C e D
author Edisio Oliveira de Azevedo
author_facet Edisio Oliveira de Azevedo
author_role author
dc.contributor.author.fl_str_mv Edisio Oliveira de Azevedo
dc.subject.por.fl_str_mv Clostridiose Vacinas
topic Clostridiose Vacinas
Toxinas
Enterotoxemia
Vacinas
C perfringens
dc.subject.other.none.fl_str_mv Toxinas
Enterotoxemia
Vacinas
C perfringens
description Seven commercial vaccines against clostridiosis. which had in their composition type C and/or D Closmdzum pemingens toxoids. have been evaluated for sterility, residual toxicity and potency. Six vaccines were produced in Brazil and one was imported, a bivalent toxoid standard of C. perjiingens type C and D has been used as a test control. All tested vaccines were sterile when cultivated in bacteria and fungi growth media. and were innocuous when administrated to mice via intraperitoneal route. ln relation to the potency, evaluated by mice semm neutralization tests using immunized rabbits sera pooled the imported vaccine and the standard toxoid have presented higher serum antibodies titres than the required minimum level of 10 and 5 IU/mL for beta and epsilon toxins produced from type C and D C. peyiirigens, respectively. The brazilian made vaccines were ineffective in stimulating serum antibodies levels specetie to beta and epilon toxins, according to the recommended test level for quality control. Four out of six brazilian made toxoids were also tested in goats but did not presefnt detectable antitoxins levels within the test level of L+ or L+/W used for beta and epsilon toxins. respectively. The toxins utilized have been produced cultivating C. pemingens within in dialisys membrane.
publishDate 1997
dc.date.issued.fl_str_mv 1997-02-17
dc.date.accessioned.fl_str_mv 2019-08-10T05:17:58Z
2025-09-09T00:09:22Z
dc.date.available.fl_str_mv 2019-08-10T05:17:58Z
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dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
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