Desenvolvimento de método LC/MS/MS para análise multirresíduo de agrotóxicos em café 

• Ano de defesa: 2012
• Autor(a) principal: Claudia Maria Dias Paes
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais
• Palavras-chave em Português: Química analítica; Análise ambiental; Produtos químicos agrícolas; Extração (Quimica); Espectrometria de massa; Cafe
• Link de acesso: https://hdl.handle.net/1843/SFSA-8YBQJP

Coffee is a major tropical agricultural commodity and represents a significant fraction to the economy of many countries. However, unwanted species of plants or animals that cause damage or interfere with the production, transport and storage of coffee can harm their trade. A solution to this is the use of pesticides, which in some cases harm to human health and the environment.Considering environmental risks and food security, it is necessary the development of performing methodologies to the determination of pesticides residues in coffee. An ideal analytical method must be able to analyze the large number of existing pesticides, without interference of the matriz, to detect and quantify unequivocally the analytes involved at low levels of concentration, accurately and with a small number of measurement that provide time-saving.This work consisted in the development of a multirresidue method for analysis of pesticides in coffee by high performance liquid chromatography coupled to mass spectrometry (LC/MS/MS). The extraction procedure used was the so-called QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe). The optimizations were carried out in order that the extraction of coffee was efficient and selective.The following parameters were evaluated: selectivity, analytical range, linearity, limit of detection (LOD), limit of quantification (LOQ), precision (RSD) and recovery of the method. 160 analytes were studied and 128 were validated. The results showed that the method is selective. The best working range was between 10.0 and 100.0 g kg-1. The method presented good linearity with determination coefficients R2 between 0.9922 and 0.9999. The sensitivity, recovery and precision were adequate for the multirresidue analysis of pesticides in coffee. The LOD varied from 0.05-18.48 ìg kg-1 and LOQ varied from 0.11-40.12 ìg kg-1 . The recovery varied between 70 and 120% with values of RSD < 20% .The method was applied to the analyses of 15 coffee samples