Microquimerismo em glândulas salivares labiais de pacientes submetidas a transplante de células tronco hematopoiéticas

Detalhes bibliográficos
Ano de defesa: 2010
Autor(a) principal: Leandro Napier de Souza
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Minas Gerais
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Patologia bucal
Glândulas salivares
Células tronco hematopoiéticas Transplante
Link de acesso: https://hdl.handle.net/1843/ZMRO-8DYMX9
Resumo: OBJECTIVE: Microchimerism represents a small portion of cells or DNA from one individual in another one. It has been extensively investigated in autoimmune diseases, which display similarities with graft-versus-host disease. The aim of this study was to investigate the presence of microchimerism in minor salivary glands of hematopoietic stem cell transplanted patients, one of the targets of graft-versus-host disease. METHODS: Labial salivary glands biopsy specimens from 11 stem cell transplanted patients were analyzed. The samples were grouped in control (5 specimens from a female-to-female transplantation) and study group (5 glands from male-to-female transplantation). One male transplanted patient was used as a positive control. Fluorescence in-situ hybridization with Y-chromosome probe and immunofluorescence anticytokeratin AE1/AE3 were used to identify Y-chromosome positive glandular epithelial cells from these patients who have received allogeneic hematopoietic stem cell transplantation. RESULTS: All samples were positive to Y-chromosome and cytokeratin AE1/AE3, in agreement with the pattern exhibited by male labial salivary gland. None of the samples from control group (female-to-female transplantation) were positive to Y-chromosome despite being positive to cytokeratin AE1/AE3. CONCLUSION: Microchimerism in the labial salivary glands of sex-mismatched stem cell transplanted patients is a real phenomenon. Further studies are necessary to elucidate the impact of this phenomenon on the clinical status of stem cell transplanted patients.
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spelling 2019-08-13T06:02:16Z2025-09-08T23:24:49Z2019-08-13T06:02:16Z2010-07-29https://hdl.handle.net/1843/ZMRO-8DYMX9OBJECTIVE: Microchimerism represents a small portion of cells or DNA from one individual in another one. It has been extensively investigated in autoimmune diseases, which display similarities with graft-versus-host disease. The aim of this study was to investigate the presence of microchimerism in minor salivary glands of hematopoietic stem cell transplanted patients, one of the targets of graft-versus-host disease. METHODS: Labial salivary glands biopsy specimens from 11 stem cell transplanted patients were analyzed. The samples were grouped in control (5 specimens from a female-to-female transplantation) and study group (5 glands from male-to-female transplantation). One male transplanted patient was used as a positive control. Fluorescence in-situ hybridization with Y-chromosome probe and immunofluorescence anticytokeratin AE1/AE3 were used to identify Y-chromosome positive glandular epithelial cells from these patients who have received allogeneic hematopoietic stem cell transplantation. RESULTS: All samples were positive to Y-chromosome and cytokeratin AE1/AE3, in agreement with the pattern exhibited by male labial salivary gland. None of the samples from control group (female-to-female transplantation) were positive to Y-chromosome despite being positive to cytokeratin AE1/AE3. CONCLUSION: Microchimerism in the labial salivary glands of sex-mismatched stem cell transplanted patients is a real phenomenon. Further studies are necessary to elucidate the impact of this phenomenon on the clinical status of stem cell transplanted patients.Universidade Federal de Minas GeraisQuimerismoTransplante de células-tronco hematopoéticasGlândulas salivares menoresPatologia bucalGlândulas salivaresCélulas tronco hematopoiéticas TransplanteMicroquimerismo em glândulas salivares labiais de pacientes submetidas a transplante de células tronco hematopoiéticasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisLeandro Napier de Souzainfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGRicardo Santiago GomezDaniela Rodrigues de Faria BarbosaCarolina Cavalieri GomesJeane de Fatima Correia SilvaJUNIA NORONHA CARVALHAISHÉLDER ANTÔNIO REBELO PONTESObjetivo: O Microquimerismo se refere a uma pequena quantidade de células ou DNA de um indivíduo presentes em outro indivíduo. O seu papel nas doenças autoimunes com semelhenças à doença do enxerto contra o hospedeiro tem sido muito estudado. Este trabalho teve como objetivo determinar se o microquimerismo do cromossomo-Y está presente em glândulas salivares labiais de mulheres submetidas a transplantes de células tronco hematopoiéticas com doadores do gênero masculino. Metodologia: onze amostras de glândulas salivares labiais de pacientes submetidos a transplante de células tronco hematopoiéticas alogênicos foram obtidas dos arquivos do Laboratório de Patologia Bucal da Faculdade de Odontologia da UFMG. Nenhum dos autores teve conhecimento prévio do grau de cGVHD das mesmas. Destas amostras, cinco eram de transplante entre gêneros feminino-feminino (grupo controle) e cinco entre masculino-feminino (grupo estudo), além de uma do gênero masculino (controle positivo). Hibridização in situ por fluorescência com sonda para cromossomo-Y e imunofluorescência para anticitoqueratina AE1/AE3 foram utilizadas para identificar células epiteliais glandulares positivas para cromossomo-Y. Resultados: Todas as pacientes do grupo estudado tiveram marcação positiva para o cromossomo-Y e para citoqueratina AE1/AE3 quando comparadas com o controle positivo. Nenhuma amostra do grupo controle se mostrou positiva para cromossomo-Y. Conclusão: O microquimerismo nas glândulas salivares labiais de pacientes submetidas a transplante de células tronco hematopoiéticas com doadores do gênero masculino é um fenômeno real.UFMGORIGINALtese_doutorado_leandro_napier_de_souza.pdfapplication/pdf13432219https://repositorio.ufmg.br//bitstreams/ab645f9d-cf66-4ced-b02b-ab0287e6a438/download07947252b036dac52c66057c2a91856eMD51trueAnonymousREADTEXTtese_doutorado_leandro_napier_de_souza.pdf.txttext/plain113572https://repositorio.ufmg.br//bitstreams/0ef9c381-5012-4784-9034-21b3fc1cfdff/download8380742768ed18007116ebc8280e8d21MD52falseAnonymousREAD1843/ZMRO-8DYMX92025-09-08 20:24:49.053open.accessoai:repositorio.ufmg.br:1843/ZMRO-8DYMX9https://repositorio.ufmg.br/Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2025-09-08T23:24:49Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Microquimerismo em glândulas salivares labiais de pacientes submetidas a transplante de células tronco hematopoiéticas
title Microquimerismo em glândulas salivares labiais de pacientes submetidas a transplante de células tronco hematopoiéticas
spellingShingle Microquimerismo em glândulas salivares labiais de pacientes submetidas a transplante de células tronco hematopoiéticas
Leandro Napier de Souza
Patologia bucal
Glândulas salivares
Células tronco hematopoiéticas Transplante
Quimerismo
Transplante de células-tronco hematopoéticas
Glândulas salivares menores
title_short Microquimerismo em glândulas salivares labiais de pacientes submetidas a transplante de células tronco hematopoiéticas
title_full Microquimerismo em glândulas salivares labiais de pacientes submetidas a transplante de células tronco hematopoiéticas
title_fullStr Microquimerismo em glândulas salivares labiais de pacientes submetidas a transplante de células tronco hematopoiéticas
title_full_unstemmed Microquimerismo em glândulas salivares labiais de pacientes submetidas a transplante de células tronco hematopoiéticas
title_sort Microquimerismo em glândulas salivares labiais de pacientes submetidas a transplante de células tronco hematopoiéticas
author Leandro Napier de Souza
author_facet Leandro Napier de Souza
author_role author
dc.contributor.author.fl_str_mv Leandro Napier de Souza
dc.subject.por.fl_str_mv Patologia bucal
Glândulas salivares
Células tronco hematopoiéticas Transplante
topic Patologia bucal
Glândulas salivares
Células tronco hematopoiéticas Transplante
Quimerismo
Transplante de células-tronco hematopoéticas
Glândulas salivares menores
dc.subject.other.none.fl_str_mv Quimerismo
Transplante de células-tronco hematopoéticas
Glândulas salivares menores
description OBJECTIVE: Microchimerism represents a small portion of cells or DNA from one individual in another one. It has been extensively investigated in autoimmune diseases, which display similarities with graft-versus-host disease. The aim of this study was to investigate the presence of microchimerism in minor salivary glands of hematopoietic stem cell transplanted patients, one of the targets of graft-versus-host disease. METHODS: Labial salivary glands biopsy specimens from 11 stem cell transplanted patients were analyzed. The samples were grouped in control (5 specimens from a female-to-female transplantation) and study group (5 glands from male-to-female transplantation). One male transplanted patient was used as a positive control. Fluorescence in-situ hybridization with Y-chromosome probe and immunofluorescence anticytokeratin AE1/AE3 were used to identify Y-chromosome positive glandular epithelial cells from these patients who have received allogeneic hematopoietic stem cell transplantation. RESULTS: All samples were positive to Y-chromosome and cytokeratin AE1/AE3, in agreement with the pattern exhibited by male labial salivary gland. None of the samples from control group (female-to-female transplantation) were positive to Y-chromosome despite being positive to cytokeratin AE1/AE3. CONCLUSION: Microchimerism in the labial salivary glands of sex-mismatched stem cell transplanted patients is a real phenomenon. Further studies are necessary to elucidate the impact of this phenomenon on the clinical status of stem cell transplanted patients.
publishDate 2010
dc.date.issued.fl_str_mv 2010-07-29
dc.date.accessioned.fl_str_mv 2019-08-13T06:02:16Z
2025-09-08T23:24:49Z
dc.date.available.fl_str_mv 2019-08-13T06:02:16Z
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
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