Efeitos da farinha liofilizada da semente de abacate (Persea americana) em camundongos alimentados com dieta hiperlipídica
Ano de defesa: | 2022 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Fundação Universidade Federal de Mato Grosso do Sul
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Programa de Pós-Graduação: |
Não Informado pela instituição
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Departamento: |
Não Informado pela instituição
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País: |
Brasil
|
Palavras-chave em Português: | |
Link de acesso: | https://repositorio.ufms.br/handle/123456789/4389 |
Resumo: | Introduction: The reduction of food waste, the use in its entirety and the adoption of efficient conservation processes, such as lyophilization, are themes that represent the basis for sustainability and food safety. In this sense, it is interesting to investigate the Persea americana, or avocado, which has increased waste by discarding the peel and seed. Some authors carried out the characterization of its seed and found the presence of biologically active compounds, which in experimentation showed hypoglycemic, hypolipidemic, anti-inflammatory and antioxidant effects, thus being able to be used as coadjuvants in the treatment of various diseases. Objectives: To evaluate the effects of freeze-dried avocado (Persea americana) seed meal in mice fed a high-fat diet. Methodology: The seeds were freeze-dried and ground to a flour point, with subsequent centesimal characterization. For the experimental design, Swiss mice were divided into control groups (Nuvilab group/Nuvital® diet; AIN93M group/ AIN-93M diet; High Fat group/ high fat diet) and experimental groups (F0.5, F1 and F2/ high fat diet with addition of freeze-dried flour at concentrations of 0.5%, 1% and 2%, respectively), evaluated for 13 weeks. The oral glucose tolerance test and insulin sensitivity test were performed, followed by euthanasia, and material was collected to quantify adiposity, fasting glucose, insulin, total cholesterol and fractions. Histological analysis of the liver, pancreas and epididymal adipose tissue was performed. Results were expressed as mean±standard error (p≤0.05). Results: In the proximate evaluation of the freeze-dried flour, the moisture content was low and the amount of total carbohydrates was high.,And also, it showed having a good amount of protein high amount of protein and high antioxidant activity from bioactive compounds, with low levels of fat. In the composition of fatty acids, there was a predominance of saturated, but a good concentration of polyunsaturated and essential acids. In the experimental design, the F0.5 group obtained greater weight gain (p<0.001) when compared to normolipidic control groups. This result also occurred in the parameter of weight of the cushions (p<0.05) and adiposity index (p<0.05). As for the lipid profile, there was no difference in the triglycerides parameter, but when analyzing total and non-HDL-c cholesterol, higher values (p<0.001) were observed in relation to controls. In the glucose profile, the highest values (p<0.001) belonged to the High Fat control and all experimental groups, similar to the results of the oral glucose tolerance test, where F2 was higher (p<0.05) than Nuvilab and AIN93M in all times, which was repeated in the insulin sensitivity test, but the difference occurred only in relation to Nuvilab (p<0.05). Regarding the area of adipocytes, there was no statistical difference in the comparison between the groups (p=0.12). There were changes in the liver analysis in the experimental groups (steatosis, lobular inflammation, ballooning, apoptosis and Mallory bodies). In the pancreas, only F1 and F2 presented hypotrophy of the islets of Langerhans. Conclusion: The freeze-dried avocado seed flour has an important composition n nutrients and bioactive compounds, but at the concentrations used, it was not possible to observe positive results in the analyzed parameters, thus requiring further studies using higher concentrations. |
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2022-02-25T03:17:24Z2022-02-25T03:17:24Z2022https://repositorio.ufms.br/handle/123456789/4389Introduction: The reduction of food waste, the use in its entirety and the adoption of efficient conservation processes, such as lyophilization, are themes that represent the basis for sustainability and food safety. In this sense, it is interesting to investigate the Persea americana, or avocado, which has increased waste by discarding the peel and seed. Some authors carried out the characterization of its seed and found the presence of biologically active compounds, which in experimentation showed hypoglycemic, hypolipidemic, anti-inflammatory and antioxidant effects, thus being able to be used as coadjuvants in the treatment of various diseases. Objectives: To evaluate the effects of freeze-dried avocado (Persea americana) seed meal in mice fed a high-fat diet. Methodology: The seeds were freeze-dried and ground to a flour point, with subsequent centesimal characterization. For the experimental design, Swiss mice were divided into control groups (Nuvilab group/Nuvital® diet; AIN93M group/ AIN-93M diet; High Fat group/ high fat diet) and experimental groups (F0.5, F1 and F2/ high fat diet with addition of freeze-dried flour at concentrations of 0.5%, 1% and 2%, respectively), evaluated for 13 weeks. The oral glucose tolerance test and insulin sensitivity test were performed, followed by euthanasia, and material was collected to quantify adiposity, fasting glucose, insulin, total cholesterol and fractions. Histological analysis of the liver, pancreas and epididymal adipose tissue was performed. Results were expressed as mean±standard error (p≤0.05). Results: In the proximate evaluation of the freeze-dried flour, the moisture content was low and the amount of total carbohydrates was high.,And also, it showed having a good amount of protein high amount of protein and high antioxidant activity from bioactive compounds, with low levels of fat. In the composition of fatty acids, there was a predominance of saturated, but a good concentration of polyunsaturated and essential acids. In the experimental design, the F0.5 group obtained greater weight gain (p<0.001) when compared to normolipidic control groups. This result also occurred in the parameter of weight of the cushions (p<0.05) and adiposity index (p<0.05). As for the lipid profile, there was no difference in the triglycerides parameter, but when analyzing total and non-HDL-c cholesterol, higher values (p<0.001) were observed in relation to controls. In the glucose profile, the highest values (p<0.001) belonged to the High Fat control and all experimental groups, similar to the results of the oral glucose tolerance test, where F2 was higher (p<0.05) than Nuvilab and AIN93M in all times, which was repeated in the insulin sensitivity test, but the difference occurred only in relation to Nuvilab (p<0.05). Regarding the area of adipocytes, there was no statistical difference in the comparison between the groups (p=0.12). There were changes in the liver analysis in the experimental groups (steatosis, lobular inflammation, ballooning, apoptosis and Mallory bodies). In the pancreas, only F1 and F2 presented hypotrophy of the islets of Langerhans. Conclusion: The freeze-dried avocado seed flour has an important composition n nutrients and bioactive compounds, but at the concentrations used, it was not possible to observe positive results in the analyzed parameters, thus requiring further studies using higher concentrations.Introdução: A redução do desperdício de alimentos, a utilização em sua totalidade e a adoção de processos eficientes de conservação, como a liofilização, são temáticas que representam a base no quesito sustentabilidade e segurança alimentar. Nesse sentido, torna-se interessante investigar a Persea americana, o qual tem elevado o desperdício ao se descartarem a casca e semente. Alguns autores realizaram a caracterização de sua semente e encontraram a presença de compostos biologicamente ativos, que em experimentação apresentaram efeitos hipoglicemiantes, hipolipidêmicos, anti-inflamatórios e antioxidantes, podendo dessa forma ser aproveitada como coadjuvantes no tratamento de diversas doenças. Objetivos: Avaliar os efeitos da farinha liofilizada da semente do abacate (Persea americana) em camundongos alimentados com dieta hiperlipídica. Metodologia: As sementes foram liofilizadas e moídas a ponto de farinha, com posterior caracterização centesimal. Para o delineamento experimental foram utilizados camundongos Swiss divididos em grupos controles (grupo Nuvilab/dieta Nuvital®; grupo AIN93M/ dieta AIN-93M; grupo High Fat/ dieta hiperlipídica) e grupos experimentais (F0,5, F1 e F2/ dieta hiperlipídica com adição de farinha liofilizada nas concentrações 0,5%, 1% e 2%, respectivamente), avaliados por 13 semanas. Foi realizado o teste de tolerância oral à glicose e teste de sensibilidade à insulina, e em seguida a eutanásia, sendo coletado material para quantificação da adiposidade, glicemia de jejum, insulina, colesterol total e frações. Foi realizada análise histológica do fígado, pâncreas e tecido adiposo epididimal. Os resultados foram expressos em média±desviopadrão (p≤0,05). Resultados: Na avaliação centesimal da farinha liofilizada foi baixa a umidade e elevada a quantidade de carboidratos totais. Apresentou boa quantidade de proteínas e alta atividade antioxidante a partir de compostos bioativos, com níveis de gordura pouco elevados. Na composição em ácidos graxos, houve predominância dos saturados, todavia boa concentração de ácidos poliinsaturados e essenciais. No delineamento experimental, o grupo F0,5 obteve maior ganho de peso (p<0,001) quando comparado aos grupos controles normolipídicos. Esse resultado também ocorreu no parâmetro de peso dos coxins (p<0,05) e índice de adiposidade (p<0,05). Quanto ao perfil lipídico, não houve diferença no parâmetro de triglicerídeos, mas ao analisar colesterol total e não-HDL-c observa-se valores maiores (p<0,001) em relação aos controles. No perfil glicídico, os maiores valores (p<0,001) pertenceram ao controle High Fat e todos os grupos experimentais, semelhante aos resultados do teste de tolerância oral a glicose, onde F2 foi maior (p<0,05) que Nuvilab e AIN93M em todos os tempos, o que se repetiu no teste de sensibilidade a insulina, porém a diferença ocorreu somente em relação ao Nuvilab (p<0,05). Em relação à área dos adipócitos não houve diferença estatística na comparação entre os grupos (p=0,12). Na análise hepática ocorreram alterações nos grupos experimentais (esteatose, inflamação lobular, balonização, apoptose e corpúsculos de Mallory). No pâncreas, somente AIN93M e F0,5 não apresentaram hipotrofia das ilhotas de langerhans. Conclusão: A farinha liofilizada da semente de abacate apresenta importante composição em nutrientes e compostos bioativos, porém nas concentrações utilizadas não foi possível observar resultados positivos nos parâmetros analisados, havendo assim necessidade de estudos posteriores que utilizem maiores concentrações.Fundação Universidade Federal de Mato Grosso do SulUFMSBrasilCamundongosDieta HiperlipídicaFarinhaPerseaDiet, High-FatFlourMiceEfeitos da farinha liofilizada da semente de abacate (Persea americana) em camundongos alimentados com dieta hiperlipídicainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisHiane, Priscila AikoMarques, Amanda Ribeiroinfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFMSinstname:Universidade Federal de Mato Grosso do Sul (UFMS)instacron:UFMSTHUMBNAILDissertação Pronta Amanda.pdf.jpgDissertação Pronta Amanda.pdf.jpgGenerated Thumbnailimage/jpeg1179https://repositorio.ufms.br/bitstream/123456789/4389/3/Disserta%c3%a7%c3%a3o%20Pronta%20Amanda.pdf.jpg4eef1ce0a75175f81f1cbf1a0dd5c5bcMD53TEXTDissertação Pronta Amanda.pdf.txtDissertação Pronta Amanda.pdf.txtExtracted texttext/plain150778https://repositorio.ufms.br/bitstream/123456789/4389/2/Disserta%c3%a7%c3%a3o%20Pronta%20Amanda.pdf.txtcde1bc0a2f72c0456a3706592c31c883MD52ORIGINALDissertação Pronta Amanda.pdfDissertação Pronta Amanda.pdfapplication/pdf2453679https://repositorio.ufms.br/bitstream/123456789/4389/1/Disserta%c3%a7%c3%a3o%20Pronta%20Amanda.pdf1ed1fe86e7d177b62e6a0ef45cd819f9MD51123456789/43892023-02-13 08:17:35.823oai:repositorio.ufms.br:123456789/4389Repositório InstitucionalPUBhttps://repositorio.ufms.br/oai/requestri.prograd@ufms.bropendoar:21242023-02-13T12:17:35Repositório Institucional da UFMS - Universidade Federal de Mato Grosso do Sul (UFMS)false |
dc.title.pt_BR.fl_str_mv |
Efeitos da farinha liofilizada da semente de abacate (Persea americana) em camundongos alimentados com dieta hiperlipídica |
title |
Efeitos da farinha liofilizada da semente de abacate (Persea americana) em camundongos alimentados com dieta hiperlipídica |
spellingShingle |
Efeitos da farinha liofilizada da semente de abacate (Persea americana) em camundongos alimentados com dieta hiperlipídica Marques, Amanda Ribeiro Camundongos Dieta Hiperlipídica Farinha Persea Diet, High-Fat Flour Mice |
title_short |
Efeitos da farinha liofilizada da semente de abacate (Persea americana) em camundongos alimentados com dieta hiperlipídica |
title_full |
Efeitos da farinha liofilizada da semente de abacate (Persea americana) em camundongos alimentados com dieta hiperlipídica |
title_fullStr |
Efeitos da farinha liofilizada da semente de abacate (Persea americana) em camundongos alimentados com dieta hiperlipídica |
title_full_unstemmed |
Efeitos da farinha liofilizada da semente de abacate (Persea americana) em camundongos alimentados com dieta hiperlipídica |
title_sort |
Efeitos da farinha liofilizada da semente de abacate (Persea americana) em camundongos alimentados com dieta hiperlipídica |
author |
Marques, Amanda Ribeiro |
author_facet |
Marques, Amanda Ribeiro |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Hiane, Priscila Aiko |
dc.contributor.author.fl_str_mv |
Marques, Amanda Ribeiro |
contributor_str_mv |
Hiane, Priscila Aiko |
dc.subject.por.fl_str_mv |
Camundongos Dieta Hiperlipídica Farinha Persea Diet, High-Fat Flour Mice |
topic |
Camundongos Dieta Hiperlipídica Farinha Persea Diet, High-Fat Flour Mice |
description |
Introduction: The reduction of food waste, the use in its entirety and the adoption of efficient conservation processes, such as lyophilization, are themes that represent the basis for sustainability and food safety. In this sense, it is interesting to investigate the Persea americana, or avocado, which has increased waste by discarding the peel and seed. Some authors carried out the characterization of its seed and found the presence of biologically active compounds, which in experimentation showed hypoglycemic, hypolipidemic, anti-inflammatory and antioxidant effects, thus being able to be used as coadjuvants in the treatment of various diseases. Objectives: To evaluate the effects of freeze-dried avocado (Persea americana) seed meal in mice fed a high-fat diet. Methodology: The seeds were freeze-dried and ground to a flour point, with subsequent centesimal characterization. For the experimental design, Swiss mice were divided into control groups (Nuvilab group/Nuvital® diet; AIN93M group/ AIN-93M diet; High Fat group/ high fat diet) and experimental groups (F0.5, F1 and F2/ high fat diet with addition of freeze-dried flour at concentrations of 0.5%, 1% and 2%, respectively), evaluated for 13 weeks. The oral glucose tolerance test and insulin sensitivity test were performed, followed by euthanasia, and material was collected to quantify adiposity, fasting glucose, insulin, total cholesterol and fractions. Histological analysis of the liver, pancreas and epididymal adipose tissue was performed. Results were expressed as mean±standard error (p≤0.05). Results: In the proximate evaluation of the freeze-dried flour, the moisture content was low and the amount of total carbohydrates was high.,And also, it showed having a good amount of protein high amount of protein and high antioxidant activity from bioactive compounds, with low levels of fat. In the composition of fatty acids, there was a predominance of saturated, but a good concentration of polyunsaturated and essential acids. In the experimental design, the F0.5 group obtained greater weight gain (p<0.001) when compared to normolipidic control groups. This result also occurred in the parameter of weight of the cushions (p<0.05) and adiposity index (p<0.05). As for the lipid profile, there was no difference in the triglycerides parameter, but when analyzing total and non-HDL-c cholesterol, higher values (p<0.001) were observed in relation to controls. In the glucose profile, the highest values (p<0.001) belonged to the High Fat control and all experimental groups, similar to the results of the oral glucose tolerance test, where F2 was higher (p<0.05) than Nuvilab and AIN93M in all times, which was repeated in the insulin sensitivity test, but the difference occurred only in relation to Nuvilab (p<0.05). Regarding the area of adipocytes, there was no statistical difference in the comparison between the groups (p=0.12). There were changes in the liver analysis in the experimental groups (steatosis, lobular inflammation, ballooning, apoptosis and Mallory bodies). In the pancreas, only F1 and F2 presented hypotrophy of the islets of Langerhans. Conclusion: The freeze-dried avocado seed flour has an important composition n nutrients and bioactive compounds, but at the concentrations used, it was not possible to observe positive results in the analyzed parameters, thus requiring further studies using higher concentrations. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-02-25T03:17:24Z |
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2022-02-25T03:17:24Z |
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2022 |
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Brasil |
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