Modulação de células do câncer de mama (MCF-7) e cocultura com nanodoses de Anacardium humile adsorvidos em microesferas de polietilenoglicol

• Ano de defesa: 2018
• Autor(a) principal: Savazzi, Suiane
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Mato Grosso; Brasil; Instituto de Ciências Exatas e da Terra (ICET) – Araguaia; UFMT CUA - Araguaia; Programa de Pós-Graduação em Ciência de Materiais
• Palavras-chave em Português: Polietilenoglicol; Cajuzinho-do-cerrado; Espécies reativas de oxigênio; Estresse oxidativo; CNPQ::CIENCIAS EXATAS E DA TERRA; Polyethylene glycol; Anacardium humile; Microspheres; Oxidative stress
• Link de acesso: http://ri.ufmt.br/handle/1/3732

Breast cancer is the most incident cancer in women worldwide after non-melanoma skin cancer. The use of microparticles for modified drugs release has been shown to be an effective alternative to enhance and/or prolong the therapeutic effect, add action target specificity and decrease toxicity. Therefore, polyethylene glycol (PEG) polymer microspheres are capable of adsorbing organic compounds, which can ensure modified release of the bioactive compounds present in medicinal plant extracts, protecting them from degradation and increasing the rate of diffusion. The aim of this work was to evaluate the oxidative stress of peripheral blood mononuclear cells (MN), cell lines of breast cancer (MCF-7) and MN and MCF-7 cell culture in the presence of ethanolic extract nanodoses (ECCC) (Anacardium humile) adsorbed onto the polyethylene glycol (PEG) microspheres. The EECC was obtained by maceration in ethyl alcohol followed by distillation. PEG microspheres 20% were prepared and then the solutions were adjusted (PEG microsphere and EECC, v.v -1 ), and incubated at 37°C for 30 minutes and were characterized by flow cytometry. Blood rheological parameters and possible changes in blood viscosity were determined in the presence of EECC adsorbed or not to PEG. Cell viability was also analyzed by counting cells in fluorescence microscopy. The evaluation of oxidative stress was determined by the release of the superoxide anion (O2- ), using the chromogen Ferricitochrome C (2 mg.mL-1 ), in two incubation times (1 and 24 h) and by the production of the enzyme superoxide dismutase (Cu- Zn-SOD) which was measured by the initial rate of reduction of Nitro Blue Tetrazolium (NBT). The antioxidant capacity of the EECC (100mg.mL-1 ) by free radical capture methods: DPPH, ABTS and FRAP, as well as the concentrations of total phenolic compounds (CFT) and total carotenoids were analyzed. The characterization revealed the PEG microspheres as spherical structures, capable of adsorbing EECC and show an average size corresponding to 6.3 µm. There was no reduction in cell viability and changes in blood viscosity. The indices of DPPH (22.0 μmol Trolox .g1 ), ABTS (45.2 μmol Trolox .g-1 ), FRAP (163.4 μmol Fe2SO4.g-1 ) carotenoids (69.0 μg β-carotene. g-1 ) and CFT (189.8 mg gallic acid.100g-1 ) were high as well as the SOD and O2 - results, indicating that EECC adsorbed or not on the microspheres of PEG was able to modulate the oxidative stress of human blood cells and co-culture MCF-7 + MN cells, acting as an effective antioxidant. Thus, it suggests a good application as a novel functional material with antitumor activity on MCF-7 cells.