Constipação induzida por loperamida em ratos : efeitos sobre trânsito alimentar e aspectos hepáticos

• Ano de defesa: 2025
• Autor(a) principal: Ali, Agrea dos Santos Ibrahim
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Mato Grosso; Brasil; Instituto de Ciências Biológicas e da Saúde (ICBS) – Araguaia; UFMT CUA - Araguaia; Programa de Pós-Graduação em Imunologia e Parasitologia Básicas e Aplicadas
• Palavras-chave em Português: BAC; Constipação; Esvaziamento gástrico; Modelo experimental; Trânsito gastrintestinal; CNPQ::CIENCIAS BIOLOGICAS; ACB; Constipation; Gastric emptying; Experimental model; Gastrointestinal transit
• Link de acesso: http://ri.ufmt.br/handle/1/7182

The gastrointestinal tract (GIT) is a complex and coordinated system, essential for digestion, nutrient absorption and waste excretion. Constipation is one of the most common gastrointestinal disorders in the world population, affecting all age groups, and its mechanisms need to be better understood. The objective of this study was to establish an experimental model of constipation in Wistar rats using loperamide hydrochloride. Male Wistar rats were divided into: control (CONT, n=12) and loperamide (LOP, n=12), which received loperamide at a dose of 10 mg/kg, orally, for 12 days of treatment. In the experiment, water and food consumption, body mass, weight, moisture and morphology of fecal pellets were determined. Gastric emptying and gastrointestinal transit records were performed by means of Alternating Current Biosusceptometry (ACB). After death, samples were collected to determine serum enzyme activities (ALT and AST) and the relative mass of the organs was also determined. Results with p ≤ 0.05 were considered statistically significant. The results showed a slowing of colonic transit, with indicators of constipation, such as a reduction in the number of fecal pellets, smaller size, and dry appearance. Additionally, there was a reduction in body mass, food consumption, and fluid intake. There was also a reduction in periepididymal fat and liver mass, although without changes in hepatic enzyme levels ALT and AST. We conclude that the proposed model of constipation induction by loperamide was successfully implemented and slowed gastrointestinal transit without apparently causing liver damage.