DESENVOLVIMENTO DE NANOESTRUTURAS CONTENDO PALMITATO DE ASCORBILA E AVALIAÇÃO DA COMPATIBILIDADE BIOLÓGICA SOBRE DIFERENTES LINHAGENS CELULARES
Ano de defesa: | 2016 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Centro Universitário Franciscano
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Nanociências
|
Departamento: |
Biociências e Nanomateriais
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/569 |
Resumo: | The ascorbyl palmitate (AP) has wide application in pharmaceutical, medical and cosmetic products, however, it is easily degraded when used as a free molecule. To overcome this problem, nanostructures have been developed. With the advancement of new products employing nanotechnology, it becomes increasingly essential that in addition to their physical and chemical activity, biological tests are carried out to assess its efficacy and safety. Thus, this study aimed to develop, characterize and determine the physical-chemical stability of nanostructures containing ascorbyl palmitate and evaluate its biological activity in vitro. Lipid nanoparticles (LNAP) and nanoemulsions (NEAP) containing ascorbyl palmitate, and lipid nanoparticles (LNBC) and nanoemulsions (NEBC) without active, have been developed and characterized, and stability was assessed for 90 days (25 ± 2 °C and 5 ± 2 °C). Inicially, the LNAP have a content of 88.90%, pH 4.34, particle diameter of 86.41 nm and zeta potential of -12.20 mV. The NEAP initially presented with a content of 101.47%, pH 4.07, particle diameter of 236.01 nm and zeta potential of -42.43 mV. The NEAP were still characterized by Transmission Electronic Microscopy (TEM), showing spherical morphology. After, they were incorporated into semi-solid bases of Carbopol® Ultrez 10 NF the active in free form (GAP) and associated with nanostructures (GLNAP and GNEAP) and stored for 90 days, for the stability study, by quantifying the content active, determination of organoleptic characteristics and pH determinations. All formulations initially presented a homogeneous aspect and slightly acid pH, around 5.14 to GLNAP, 5.29 to GNEAP and 4.61 to GAP. We obtained a initial concentration of active to GLNAP of 84.89%, to GNEAP of 97.03% and to GPA of 100.48%. However, we observed a decrease in both formulations, in different temperatures, after 90 days of analysis; where at the end of this period, the GNEAP remained with a higher concentration of AP. Finally, we assessed cell viability (MTT), in peripheral blood mononuclear cells (PBMC), in VERO and B16F10 cell; genotoxicity (mitotic index and nuclear anomalies) in PBMC and melanin content in B16F10, for samples of NEAP, NEBC and free AP, in concentrations of 25; 50; 100 and 200 μM. The cell viability assay (MTT) were expressed as percentage of control. The results of the MTT assay after 72h, showed no reduction in cell viability for all concentrations tested for the NEAP, NEBC and free AP, in PBMC. To VERO and B16F10 cells there was a decrease in cell viability for NEAP and NEBC, at concentrations of 100 and 200 μM. By microscopic analysis did not metanucleares changes and chromosomal instabilities in concentrations of NEAP, NEBC and AP free, thus not showing the genotoxicity test applied were verified. According to the present results, it can be concluded that the NEAP showed a higher partial protection to the AP against the instability, and that the nanostructure non-cytotoxic. There was no chromosomal instability in the samples, not showing so genotoxicity. |
id |
UFN-1_e1cba18651a9f5d9a7dedd70a2a45f9a |
---|---|
oai_identifier_str |
oai:tede.universidadefranciscana.edu.br:UFN-BDTD/569 |
network_acronym_str |
UFN-1 |
network_name_str |
Repositório Institucional Universidade Franciscana |
repository_id_str |
|
spelling |
Rodrigues Junior, Luiz CarlosAlves, Marta PalmaRodrigues, Oscar Endrigo DornelesSilva Júnior, Flávio Manoel Rodrigues daOurique, Aline FerreiraFernandes, Lianda da SilvaSilva, Ana Paula Tasquetto da2018-08-17T20:54:40Z2016-07-26Silva, Ana Paula Tasquetto da. DESENVOLVIMENTO DE NANOESTRUTURAS CONTENDO PALMITATO DE ASCORBILA E AVALIAÇÃO DA COMPATIBILIDADE BIOLÓGICA SOBRE DIFERENTES LINHAGENS CELULARES. 2016. 132f. Tese( Programa de Pós-Graduação em Nanociências) - Centro Universitário Franciscano, Santa Maria - RS .http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/569The ascorbyl palmitate (AP) has wide application in pharmaceutical, medical and cosmetic products, however, it is easily degraded when used as a free molecule. To overcome this problem, nanostructures have been developed. With the advancement of new products employing nanotechnology, it becomes increasingly essential that in addition to their physical and chemical activity, biological tests are carried out to assess its efficacy and safety. Thus, this study aimed to develop, characterize and determine the physical-chemical stability of nanostructures containing ascorbyl palmitate and evaluate its biological activity in vitro. Lipid nanoparticles (LNAP) and nanoemulsions (NEAP) containing ascorbyl palmitate, and lipid nanoparticles (LNBC) and nanoemulsions (NEBC) without active, have been developed and characterized, and stability was assessed for 90 days (25 ± 2 °C and 5 ± 2 °C). Inicially, the LNAP have a content of 88.90%, pH 4.34, particle diameter of 86.41 nm and zeta potential of -12.20 mV. The NEAP initially presented with a content of 101.47%, pH 4.07, particle diameter of 236.01 nm and zeta potential of -42.43 mV. The NEAP were still characterized by Transmission Electronic Microscopy (TEM), showing spherical morphology. After, they were incorporated into semi-solid bases of Carbopol® Ultrez 10 NF the active in free form (GAP) and associated with nanostructures (GLNAP and GNEAP) and stored for 90 days, for the stability study, by quantifying the content active, determination of organoleptic characteristics and pH determinations. All formulations initially presented a homogeneous aspect and slightly acid pH, around 5.14 to GLNAP, 5.29 to GNEAP and 4.61 to GAP. We obtained a initial concentration of active to GLNAP of 84.89%, to GNEAP of 97.03% and to GPA of 100.48%. However, we observed a decrease in both formulations, in different temperatures, after 90 days of analysis; where at the end of this period, the GNEAP remained with a higher concentration of AP. Finally, we assessed cell viability (MTT), in peripheral blood mononuclear cells (PBMC), in VERO and B16F10 cell; genotoxicity (mitotic index and nuclear anomalies) in PBMC and melanin content in B16F10, for samples of NEAP, NEBC and free AP, in concentrations of 25; 50; 100 and 200 μM. The cell viability assay (MTT) were expressed as percentage of control. The results of the MTT assay after 72h, showed no reduction in cell viability for all concentrations tested for the NEAP, NEBC and free AP, in PBMC. To VERO and B16F10 cells there was a decrease in cell viability for NEAP and NEBC, at concentrations of 100 and 200 μM. By microscopic analysis did not metanucleares changes and chromosomal instabilities in concentrations of NEAP, NEBC and AP free, thus not showing the genotoxicity test applied were verified. According to the present results, it can be concluded that the NEAP showed a higher partial protection to the AP against the instability, and that the nanostructure non-cytotoxic. There was no chromosomal instability in the samples, not showing so genotoxicity.O palmitato de ascorbila (PA) tem ampla aplicação em produtos farmacêuticos, médicos e cosméticos, no entanto, é facilmente degradado quando utilizado como uma molécula livre. Para superar este problema, nanoestruturas têm sido desenvolvidas. Com o avanço de novos produtos empregando a nanotecnologia, torna-se cada vez mais essencial que, além das suas propriedades físico-químicas, ensaios biológicos sejam realizados a fim de avaliar sua eficácia e segurança. Desta forma, o presente trabalho teve como objetivo desenvolver, caracterizar e determinar a estabilidade físico-química de nanoestruturas contendo palmitato de ascorbila e avaliar sua atividade biológica in vitro. Foram desenvolvidas e caracterizadas nanopartículas lipídicas (NLPA) e nanoemulsões (NEPA) contendo palmitato de ascorbila e nanopartículas lipídicas (NLBC) e nanoemulsões (NEBC) sem o ativo, sendo que a estabilidade foi avaliada durante 90 dias (25 ºC ± 2 ºC e 5 ºC ± 2 ºC). As NLPA apresentaram inicialmente um teor de 88,90%, pH 4,34, diâmetro de partícula de 86,41 nm e potencial zeta de -12,20 mV. As NEPA apresentaram inicialmente um teor de 101,47%, pH 4,07, diâmetro de partícula de 236,01 nm e potencial zeta de -42,43 mV. As NEPA ainda foram caracterizadas por Microscopia Eletrônica de Transmissão (MET), demonstrando morfologia esférica. Posteriormente, foram incorporados em bases semissólidas de gel de Carbopol® Ultrez 10 NF o ativo na forma livre (GPA) e associado às nanoestruturas (GNLPA e GNEPA), sendo armazenados, por 90 dias, para estudo de estabilidade, através da quantificação do teor de ativo, determinação das características organolépticas e determinações de pH. Todas as formulações apresentaram inicialmente um aspecto homogêneo e pH levemente ácido, em torno de 5,14 para o GNLPA, 5,29 para o GNEPA e 4,61 para o GPA. Obteve-se uma concentração inicial de ativo para o GNLPA de 84,89%, para o GNEPA de 97,03%, e para o GPA de 100,48%. Contudo, foi possível observar uma diminuição em ambas as formulações, nas diferentes temperaturas, após 90 dias de análise; sendo que, ao final deste período, o GNEPA permaneceu com uma maior concentração de PA. Por fim, foram avaliados a viabilidade celular (MTT), em células mononucleares de sangue periférico (CMSP), células VERO e B16F10; genotoxicidade (índice mitótico e anomalias nucleares) em CMSP e o conteúdo de melanina, em B16F10, para as amostras de NEPA, NEBC e PA livre, nas concentrações de 25; 50; 100 e 200 μM. O ensaio de viabilidade celular (MTT) foi expresso em porcentagem do controle. Os resultados decorrentes do ensaio MTT, após 72 h, não demonstraram redução da viabilidade celular para todas as concentrações testadas de NEPA, NEBC e PA livre, em CMSP. Para as células VERO e B16F10 houve uma diminuição na viabilidade celular com as NEPA e NEBC, nas concentrações de 100 e 200 μM. Pela análise microscópica, não foram verificadas alterações metanucleares e instabilidades cromossômicas nas concentrações testadas das NEPA, NEBC e PA livre, não evidenciando assim genotoxicidade pelo teste aplicado. De acordo com os resultados obtidos neste trabalho, pode-se concluir que as NEPA demonstraram uma maior proteção parcial ao PA frente à instabilidade, bem como não demonstraram efeitos citotóxicos. Não foi verificada instabilidade cromossômica nas amostras, não evidenciando assim, genotoxicidade.Submitted by MARCIA ROVADOSCHI (marciar@unifra.br) on 2018-08-17T20:54:40Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Tese_AnaPaulaTasquettodaSilva.pdf: 2539140 bytes, checksum: 82d4a7a56c7dc9a95260cfcf38baddca (MD5)Made available in DSpace on 2018-08-17T20:54:40Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Tese_AnaPaulaTasquettodaSilva.pdf: 2539140 bytes, checksum: 82d4a7a56c7dc9a95260cfcf38baddca (MD5) Previous issue date: 2016-07-26application/pdfhttp://www.tede.universidadefranciscana.edu.br:8080/retrieve/2250/Tese_AnaPaulaTasquettodaSilva.pdf.jpgporCentro Universitário FranciscanoPrograma de Pós-Graduação em NanociênciasUNIFRABrasilBiociências e Nanomateriaishttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessavaliação biológica, estabilidade, nanopartículas lipídicas, nanoemulsãobiological assay, stability, lipid nanoparticles, nanoemulsionBiociências e NanomateriaisDESENVOLVIMENTO DE NANOESTRUTURAS CONTENDO PALMITATO DE ASCORBILA E AVALIAÇÃO DA COMPATIBILIDADE BIOLÓGICA SOBRE DIFERENTES LINHAGENS CELULARESinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisreponame:Repositório Institucional Universidade Franciscanainstname:Universidade Franciscana (UFN)instacron:UFNLICENSElicense.txtlicense.txttext/plain; charset=utf-8309http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/569/1/license.txte4ae80c7384074d77a55dabcdffdf13aMD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/569/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/569/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/569/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54ORIGINALTese_AnaPaulaTasquettodaSilva.pdfTese_AnaPaulaTasquettodaSilva.pdfapplication/pdf2539140http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/569/5/Tese_AnaPaulaTasquettodaSilva.pdf82d4a7a56c7dc9a95260cfcf38baddcaMD55THUMBNAILTese_AnaPaulaTasquettodaSilva.pdf.jpgTese_AnaPaulaTasquettodaSilva.pdf.jpgimage/jpeg3510http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/569/6/Tese_AnaPaulaTasquettodaSilva.pdf.jpga2baf806b38cc9535fd60dd00bf0ee1eMD56TEXTTese_AnaPaulaTasquettodaSilva.pdf.txtTese_AnaPaulaTasquettodaSilva.pdf.txttext/plain288766http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/569/7/Tese_AnaPaulaTasquettodaSilva.pdf.txt4a26b14091ae14b0d548aa8430c5c605MD57UFN-BDTD/5692018-08-19 01:00:44.063oai:tede.universidadefranciscana.edu.br:UFN-BDTD/569RXN0ZSB0cmFiYWxobyBzZXLDoSBsaWNlbmNpYWRvIHNvYiBhIExpY2Vuw6dhIEF0cmlidWnDp8Ojby1Ow6NvQ29tZXJjaWFsLVNlbURlcml2YcOnw7VlcyA0LjAgSW50ZXJuYWNpb25hbCBDcmVhdGl2ZSBDb21tb25zLiBQYXJhIHZpc3VhbGl6YXIgdW1hIGPDs3BpYSBkZXN0YSBsaWNlbsOnYSwgdmlzaXRlIGh0dHA6Ly9jcmVhdGl2ZWNvbW1vbnMub3JnL2xpY2Vuc2VzL2J5LW5jLW5kLzQuMC8gb3UgbWFuZGUgdW1hIGNhcnRhIHBhcmEgQ3JlYXRpdmUgQ29tbW9ucywgUE8gQm94IDE4NjYsIE1vdW50YWluIFZpZXcsIENBIDk0MDQyLCBVU0EuRepositório de Publicaçõeshttp://www.tede.universidadefranciscana.edu.br:8080/http://www.tede.universidadefranciscana.edu.br:8080/oai/requestopendoar:2018-08-19T04:00:44Repositório Institucional Universidade Franciscana - Universidade Franciscana (UFN)false |
dc.title.por.fl_str_mv |
DESENVOLVIMENTO DE NANOESTRUTURAS CONTENDO PALMITATO DE ASCORBILA E AVALIAÇÃO DA COMPATIBILIDADE BIOLÓGICA SOBRE DIFERENTES LINHAGENS CELULARES |
title |
DESENVOLVIMENTO DE NANOESTRUTURAS CONTENDO PALMITATO DE ASCORBILA E AVALIAÇÃO DA COMPATIBILIDADE BIOLÓGICA SOBRE DIFERENTES LINHAGENS CELULARES |
spellingShingle |
DESENVOLVIMENTO DE NANOESTRUTURAS CONTENDO PALMITATO DE ASCORBILA E AVALIAÇÃO DA COMPATIBILIDADE BIOLÓGICA SOBRE DIFERENTES LINHAGENS CELULARES Silva, Ana Paula Tasquetto da avaliação biológica, estabilidade, nanopartículas lipídicas, nanoemulsão biological assay, stability, lipid nanoparticles, nanoemulsion Biociências e Nanomateriais |
title_short |
DESENVOLVIMENTO DE NANOESTRUTURAS CONTENDO PALMITATO DE ASCORBILA E AVALIAÇÃO DA COMPATIBILIDADE BIOLÓGICA SOBRE DIFERENTES LINHAGENS CELULARES |
title_full |
DESENVOLVIMENTO DE NANOESTRUTURAS CONTENDO PALMITATO DE ASCORBILA E AVALIAÇÃO DA COMPATIBILIDADE BIOLÓGICA SOBRE DIFERENTES LINHAGENS CELULARES |
title_fullStr |
DESENVOLVIMENTO DE NANOESTRUTURAS CONTENDO PALMITATO DE ASCORBILA E AVALIAÇÃO DA COMPATIBILIDADE BIOLÓGICA SOBRE DIFERENTES LINHAGENS CELULARES |
title_full_unstemmed |
DESENVOLVIMENTO DE NANOESTRUTURAS CONTENDO PALMITATO DE ASCORBILA E AVALIAÇÃO DA COMPATIBILIDADE BIOLÓGICA SOBRE DIFERENTES LINHAGENS CELULARES |
title_sort |
DESENVOLVIMENTO DE NANOESTRUTURAS CONTENDO PALMITATO DE ASCORBILA E AVALIAÇÃO DA COMPATIBILIDADE BIOLÓGICA SOBRE DIFERENTES LINHAGENS CELULARES |
author |
Silva, Ana Paula Tasquetto da |
author_facet |
Silva, Ana Paula Tasquetto da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Rodrigues Junior, Luiz Carlos |
dc.contributor.advisor-co1.fl_str_mv |
Alves, Marta Palma |
dc.contributor.referee1.fl_str_mv |
Rodrigues, Oscar Endrigo Dorneles |
dc.contributor.referee2.fl_str_mv |
Silva Júnior, Flávio Manoel Rodrigues da |
dc.contributor.referee3.fl_str_mv |
Ourique, Aline Ferreira |
dc.contributor.referee4.fl_str_mv |
Fernandes, Lianda da Silva |
dc.contributor.author.fl_str_mv |
Silva, Ana Paula Tasquetto da |
contributor_str_mv |
Rodrigues Junior, Luiz Carlos Alves, Marta Palma Rodrigues, Oscar Endrigo Dorneles Silva Júnior, Flávio Manoel Rodrigues da Ourique, Aline Ferreira Fernandes, Lianda da Silva |
dc.subject.por.fl_str_mv |
avaliação biológica, estabilidade, nanopartículas lipídicas, nanoemulsão |
topic |
avaliação biológica, estabilidade, nanopartículas lipídicas, nanoemulsão biological assay, stability, lipid nanoparticles, nanoemulsion Biociências e Nanomateriais |
dc.subject.eng.fl_str_mv |
biological assay, stability, lipid nanoparticles, nanoemulsion |
dc.subject.cnpq.fl_str_mv |
Biociências e Nanomateriais |
description |
The ascorbyl palmitate (AP) has wide application in pharmaceutical, medical and cosmetic products, however, it is easily degraded when used as a free molecule. To overcome this problem, nanostructures have been developed. With the advancement of new products employing nanotechnology, it becomes increasingly essential that in addition to their physical and chemical activity, biological tests are carried out to assess its efficacy and safety. Thus, this study aimed to develop, characterize and determine the physical-chemical stability of nanostructures containing ascorbyl palmitate and evaluate its biological activity in vitro. Lipid nanoparticles (LNAP) and nanoemulsions (NEAP) containing ascorbyl palmitate, and lipid nanoparticles (LNBC) and nanoemulsions (NEBC) without active, have been developed and characterized, and stability was assessed for 90 days (25 ± 2 °C and 5 ± 2 °C). Inicially, the LNAP have a content of 88.90%, pH 4.34, particle diameter of 86.41 nm and zeta potential of -12.20 mV. The NEAP initially presented with a content of 101.47%, pH 4.07, particle diameter of 236.01 nm and zeta potential of -42.43 mV. The NEAP were still characterized by Transmission Electronic Microscopy (TEM), showing spherical morphology. After, they were incorporated into semi-solid bases of Carbopol® Ultrez 10 NF the active in free form (GAP) and associated with nanostructures (GLNAP and GNEAP) and stored for 90 days, for the stability study, by quantifying the content active, determination of organoleptic characteristics and pH determinations. All formulations initially presented a homogeneous aspect and slightly acid pH, around 5.14 to GLNAP, 5.29 to GNEAP and 4.61 to GAP. We obtained a initial concentration of active to GLNAP of 84.89%, to GNEAP of 97.03% and to GPA of 100.48%. However, we observed a decrease in both formulations, in different temperatures, after 90 days of analysis; where at the end of this period, the GNEAP remained with a higher concentration of AP. Finally, we assessed cell viability (MTT), in peripheral blood mononuclear cells (PBMC), in VERO and B16F10 cell; genotoxicity (mitotic index and nuclear anomalies) in PBMC and melanin content in B16F10, for samples of NEAP, NEBC and free AP, in concentrations of 25; 50; 100 and 200 μM. The cell viability assay (MTT) were expressed as percentage of control. The results of the MTT assay after 72h, showed no reduction in cell viability for all concentrations tested for the NEAP, NEBC and free AP, in PBMC. To VERO and B16F10 cells there was a decrease in cell viability for NEAP and NEBC, at concentrations of 100 and 200 μM. By microscopic analysis did not metanucleares changes and chromosomal instabilities in concentrations of NEAP, NEBC and AP free, thus not showing the genotoxicity test applied were verified. According to the present results, it can be concluded that the NEAP showed a higher partial protection to the AP against the instability, and that the nanostructure non-cytotoxic. There was no chromosomal instability in the samples, not showing so genotoxicity. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-07-26 |
dc.date.accessioned.fl_str_mv |
2018-08-17T20:54:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Silva, Ana Paula Tasquetto da. DESENVOLVIMENTO DE NANOESTRUTURAS CONTENDO PALMITATO DE ASCORBILA E AVALIAÇÃO DA COMPATIBILIDADE BIOLÓGICA SOBRE DIFERENTES LINHAGENS CELULARES. 2016. 132f. Tese( Programa de Pós-Graduação em Nanociências) - Centro Universitário Franciscano, Santa Maria - RS . |
dc.identifier.uri.fl_str_mv |
http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/569 |
identifier_str_mv |
Silva, Ana Paula Tasquetto da. DESENVOLVIMENTO DE NANOESTRUTURAS CONTENDO PALMITATO DE ASCORBILA E AVALIAÇÃO DA COMPATIBILIDADE BIOLÓGICA SOBRE DIFERENTES LINHAGENS CELULARES. 2016. 132f. Tese( Programa de Pós-Graduação em Nanociências) - Centro Universitário Franciscano, Santa Maria - RS . |
url |
http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/569 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Centro Universitário Franciscano |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Nanociências |
dc.publisher.initials.fl_str_mv |
UNIFRA |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Biociências e Nanomateriais |
publisher.none.fl_str_mv |
Centro Universitário Franciscano |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional Universidade Franciscana instname:Universidade Franciscana (UFN) instacron:UFN |
instname_str |
Universidade Franciscana (UFN) |
instacron_str |
UFN |
institution |
UFN |
reponame_str |
Repositório Institucional Universidade Franciscana |
collection |
Repositório Institucional Universidade Franciscana |
bitstream.url.fl_str_mv |
http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/569/1/license.txt http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/569/2/license_url http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/569/3/license_text http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/569/4/license_rdf http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/569/5/Tese_AnaPaulaTasquettodaSilva.pdf http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/569/6/Tese_AnaPaulaTasquettodaSilva.pdf.jpg http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/569/7/Tese_AnaPaulaTasquettodaSilva.pdf.txt |
bitstream.checksum.fl_str_mv |
e4ae80c7384074d77a55dabcdffdf13a 4afdbb8c545fd630ea7db775da747b2f d41d8cd98f00b204e9800998ecf8427e d41d8cd98f00b204e9800998ecf8427e 82d4a7a56c7dc9a95260cfcf38baddca a2baf806b38cc9535fd60dd00bf0ee1e 4a26b14091ae14b0d548aa8430c5c605 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional Universidade Franciscana - Universidade Franciscana (UFN) |
repository.mail.fl_str_mv |
|
_version_ |
1797147544064622592 |