Atividade de inibição de protease da lectina de sementes de Abelmoschus esculentus l. (Moench) e seus efeitos sobre Ceratitis capitata, Meloidogyne javanica e Meloidogyne incognita

• Ano de defesa: 2015
• Autor(a) principal: Lacerda, José Thalles Jocelino Gomes de
• Orientador(a): Santi-Gadelha, Tatiane
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal da Paraíba
• Programa de Pós-Graduação: Programa de Pós-Graduação em Biologia Celular e Molecular
• Departamento: Biologia Celular e Molecular
• País: Brasil
• Palavras-chave em Português: Lectina Abelmoschus esculentus - AEL; Atividade catalítica; Ceratitis capitata; Nematóide de galha; Fungos
• Palavras-chave em Inglês: Lectin Abelmoschus esculentus - AEL; Catalytic activity; Ceratitis capitata; Root knot nematodes; Fungi
• Área do conhecimento CNPq: CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: https://repositorio.ufpb.br/jspui/handle/tede/9444

The lectins are proteins capable of recognize and bond reversibly to carbohydrates without modifying them by a non-catalytic domain. In vegetables, they are synthetized as resistance factors against phytopathogens, and since they recognize structures present in the parasites, for their structural, catalytic and carbohydrate-specific characteristics give information on the action mechanisms against different parasitic species. Our study feature its catalytic activity of the lectin extracted from Abelmoschus esculentus (AEL) and evaluated their effects on phytophagous and phytopathogens for its application as a potential as crop protection and. We performed peptide analysis from AEL using mass spectrometry to determine conservative domains inherent to its molecular properties and protease activity inhibition toxicity, essays toxicity on the fruit fly Ceratitis capitata and root knot nematodes Meloidogyne javanica and Meloidogyne incognita on their different development stages through topical application, fungistatic activity on phytopathogenic fungi. AEL presents in its composition regions that are homologous to kunitz-type protease inhibitors, with the identification of conserved peptides for this class of proteins and protease inhibition activity (92%), which reveal its catalytic function and direct their action mechanisms on the different phytopathogens. It´s displayed insecticide effect on 3rd instar larvae and pupae, nematicide effect for the root knot nematodes acting mainly in larve hatched but did not presented fungistatic activity.