Similaridade genética padrões de resistência antimicrobiana de Staphylococcus aureus de equinos sadios em diferentes regiões do Brasil

• Ano de defesa: 2015
• Autor(a) principal: Saraiva, Mauro de Mesquita Souza
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal da Paraíba; Brasil; Zootecnia; Programa de Pós-Graduação em Ciência Animal; UFPB
• Palavras-chave em Português: Identificação de Staphylococcus; MALDI-TOF; MRSA; Multirresistencia; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
• Link de acesso: https://repositorio.ufpb.br/jspui/handle/123456789/15343

Staphylococcus aureus is a major pathogen in veterinary medicine and the increasing emergence of strains resistant to different classes of antimicrobial agents elevates the need for accurate identification and monitoring of this bacterial species. This study aimed to initially evaluate the accuracy of PCR for nuc, femA and coa genes for identification of S. aureus obtained from different animal species samples. A total of 71 Staphylococcus spp. isolates were analyzed through the MALDI-TOF and were identified 12 different species. Among Staphylococcus aureus (34) the femA, nuc and coa genes were identified in 30, 26 and 16 isolates respectively. Although the specificity of the PCR for all markers tested was 100%, the sensitivity was found 88.2%, 76.5% and 46.5% for femA, nuc and coa markers, respectively. The sensitivity was 100% when used the femA and nuc markers simultaneously. The results confirm the PCR technique as a accurate for S. aureus identification and suggests the simultaneous use of femA and nuc primers. Later aimed to evaluate the antimicrobial resistance patterns and genotypic relationship between S. aureus cultured from nasal cavities of healthy horses from two geographically distant states of Brazil (Paraíba and Rio Grande do Sul). A total of 123 Staphylococcus spp. analyzed initially, 21 isolates were identified as Staphylococcus aureus by biochemical tests and PCR using species-specific markers (nuc, femA and coa). The results showed four different resistance patterns. Multidrug resistance was observed in 10 S. aureus isolates. Although none S. aureus has been found harboring mecA gene, 6 methicillin-resistant isolates were found, indicating the presence of methicillin-resistant Staphylococcus aureus (MRSA). Genotyping by Rep-PCR showed that the isolates were not grouped by geographical origin, and indicate an enormous diversity of Staphylococcus aureus strains colonizing healthy horses in Brazil.