Análise do efeito imunomodulador da ouabaína na inflamação e nocicepção

Detalhes bibliográficos
Ano de defesa: 2011
Autor(a) principal: Vasconcelos, Danielle Ingrid Bezerra de lattes
Orientador(a): Mascarenhas, Sandra Rodrigues lattes
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal da Paraí­ba
Programa de Pós-Graduação: Programa de Pós-Graduação em Biologia Celular e Molecular
Departamento: Biologia Celular e Molecular
País: Brasil
Palavras-chave em Português:
Ouabaína
Anti-inflamatória
Anti-nociceptiva
Palavras-chave em Inglês:
Ouabain
Antiinflammatory
Antinociceptive
Área do conhecimento CNPq:
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
Link de acesso: https://repositorio.ufpb.br/jspui/handle/tede/3640
Resumo: Ouabain, known as a cardiotonic glycoside capable of inhibiting the Na+/K+ ATPase, was widely used for heart failure treatment. Identified as an endogenous substance, ouabain is capable of interfering with various physiological functions, including immune system modulation. Besides that, little is known about the involvement of this substance in nociceptive and inflammatory processes. The present study investigated the role of ouabain in acute peripheral inflammation induced by intraplantar administrartion of different phlogistic agents (carrageenan, compound 48/80, histamine, bradykinin, and PGE2) and in nociceptive processes (abdominal writhing induced by acetic acid and hot plate). Ouabain produced a significant reduction in the mouse paw edema induced by carrageenan and compound 48/80. This antiinflammatory effect of ouabain is associated to the inhibition of PGE2, bradykinin, and mast-cell degranulation, but not to histamine. Ouabain also presented a central and peripheral anti-nociceptive activity. This analgesic potential might be related to the inhibition of inflammatory mediators and to activation of opioid receptors, since it was reversed by naloxone, an opioid antagonist. Additionally, the analgesic effect of ouabain was not related to sedative effect or to motor function. Taken together, the present work demonstrated for the first time, in vivo, the antiinflammatory and analgesic potential of ouabain
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spelling 2015-04-01T14:16:00Z2018-07-20T23:35:51Z2012-01-192018-07-20T23:35:51Z2011-09-30VASCONCELOS, Danielle Ingrid Bezerra de. Análise do efeito imunomodulador da ouabaína na inflamação e nocicepção. 2011. 144 f. Dissertação (Mestrado em Biologia Celular e Molecular) - Universidade Federal da Paraí­ba, João Pessoa, 2011.https://repositorio.ufpb.br/jspui/handle/tede/3640Ouabain, known as a cardiotonic glycoside capable of inhibiting the Na+/K+ ATPase, was widely used for heart failure treatment. Identified as an endogenous substance, ouabain is capable of interfering with various physiological functions, including immune system modulation. Besides that, little is known about the involvement of this substance in nociceptive and inflammatory processes. The present study investigated the role of ouabain in acute peripheral inflammation induced by intraplantar administrartion of different phlogistic agents (carrageenan, compound 48/80, histamine, bradykinin, and PGE2) and in nociceptive processes (abdominal writhing induced by acetic acid and hot plate). Ouabain produced a significant reduction in the mouse paw edema induced by carrageenan and compound 48/80. This antiinflammatory effect of ouabain is associated to the inhibition of PGE2, bradykinin, and mast-cell degranulation, but not to histamine. Ouabain also presented a central and peripheral anti-nociceptive activity. This analgesic potential might be related to the inhibition of inflammatory mediators and to activation of opioid receptors, since it was reversed by naloxone, an opioid antagonist. Additionally, the analgesic effect of ouabain was not related to sedative effect or to motor function. Taken together, the present work demonstrated for the first time, in vivo, the antiinflammatory and analgesic potential of ouabainA Ouabaína é um glicosídeo cardiotônico, inibidor da Na+/K+-ATPase, utilizada na clínica para o tratamento de insuficiência cardíaca. Atualmente, sabe-se que essa substância é endógena, e capaz de interferir em várias funções fisiológicas, inclusive em diversos aspectos do sistema imunológico. Apesar disso, pouco se sabe sobre seu envolvimento em processos inflamatórios e nociceptivos. Neste trabalho, foi avaliada a atividade da Ouabaína na inflamação aguda desencadeada pela administração de diversos agentes flogísticos (carragenina, composto 48/80, histamina, PGE2 e bradicinina) e em modelos nociceptivos (contorções abdominais induzidas por ácido acético e placa quente). A Ouabaína produziu uma redução no edema de pata produzido por carragenina e pelo composto 48/80. Esse potencial anti-inflamatório está relacionado ao bloqueio da degranulação de mastócitos, bem como pela inibição da via da PGE2 e da bradicinina, porém é independente da via da histamina. A Ouabaína também apresentou uma atividade anti-nociceptiva central e periférica. Esse efeito está vinculado à inibição da via dos mediadores inflamatórios e a mecanismos opióides, visto que foi revertido pela administração da naloxona, um inibidor dos receptores opióides. Adicionalmente, foi descrito que a inibição da dor pela Ouabaína não possui envolvimento com sedação ou diminuição da capacidade motora. O conjunto desses dados demonstra pela primeira vez, in vivo, a atividade anti-inflamatória e anti-nociceptiva da Ouabaína.Made available in DSpace on 2015-04-01T14:16:00Z (GMT). No. of bitstreams: 1 arquivototal.pdf: 5574506 bytes, checksum: a2d785f5339e8cb5e573693458ab5abd (MD5) Previous issue date: 2011-09-30Made available in DSpace on 2018-07-20T23:35:51Z (GMT). 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dc.title.por.fl_str_mv Análise do efeito imunomodulador da ouabaína na inflamação e nocicepção
title Análise do efeito imunomodulador da ouabaína na inflamação e nocicepção
spellingShingle Análise do efeito imunomodulador da ouabaína na inflamação e nocicepção
Vasconcelos, Danielle Ingrid Bezerra de
Ouabaína
Anti-inflamatória
Anti-nociceptiva
Ouabain
Antiinflammatory
Antinociceptive
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
title_short Análise do efeito imunomodulador da ouabaína na inflamação e nocicepção
title_full Análise do efeito imunomodulador da ouabaína na inflamação e nocicepção
title_fullStr Análise do efeito imunomodulador da ouabaína na inflamação e nocicepção
title_full_unstemmed Análise do efeito imunomodulador da ouabaína na inflamação e nocicepção
title_sort Análise do efeito imunomodulador da ouabaína na inflamação e nocicepção
author Vasconcelos, Danielle Ingrid Bezerra de
author_facet Vasconcelos, Danielle Ingrid Bezerra de
author_role author
dc.contributor.advisor1.fl_str_mv Mascarenhas, Sandra Rodrigues
dc.contributor.advisor1ID.fl_str_mv CPF:05334211799
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/4300081489772959
dc.contributor.authorID.fl_str_mv CPF:06450256400
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/5631429899648107
dc.contributor.author.fl_str_mv Vasconcelos, Danielle Ingrid Bezerra de
contributor_str_mv Mascarenhas, Sandra Rodrigues
dc.subject.por.fl_str_mv Ouabaína
Anti-inflamatória
Anti-nociceptiva
topic Ouabaína
Anti-inflamatória
Anti-nociceptiva
Ouabain
Antiinflammatory
Antinociceptive
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
dc.subject.eng.fl_str_mv Ouabain
Antiinflammatory
Antinociceptive
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
description Ouabain, known as a cardiotonic glycoside capable of inhibiting the Na+/K+ ATPase, was widely used for heart failure treatment. Identified as an endogenous substance, ouabain is capable of interfering with various physiological functions, including immune system modulation. Besides that, little is known about the involvement of this substance in nociceptive and inflammatory processes. The present study investigated the role of ouabain in acute peripheral inflammation induced by intraplantar administrartion of different phlogistic agents (carrageenan, compound 48/80, histamine, bradykinin, and PGE2) and in nociceptive processes (abdominal writhing induced by acetic acid and hot plate). Ouabain produced a significant reduction in the mouse paw edema induced by carrageenan and compound 48/80. This antiinflammatory effect of ouabain is associated to the inhibition of PGE2, bradykinin, and mast-cell degranulation, but not to histamine. Ouabain also presented a central and peripheral anti-nociceptive activity. This analgesic potential might be related to the inhibition of inflammatory mediators and to activation of opioid receptors, since it was reversed by naloxone, an opioid antagonist. Additionally, the analgesic effect of ouabain was not related to sedative effect or to motor function. Taken together, the present work demonstrated for the first time, in vivo, the antiinflammatory and analgesic potential of ouabain
publishDate 2011
dc.date.issued.fl_str_mv 2011-09-30
dc.date.available.fl_str_mv 2012-01-19
2018-07-20T23:35:51Z
dc.date.accessioned.fl_str_mv 2015-04-01T14:16:00Z
2018-07-20T23:35:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv VASCONCELOS, Danielle Ingrid Bezerra de. Análise do efeito imunomodulador da ouabaína na inflamação e nocicepção. 2011. 144 f. Dissertação (Mestrado em Biologia Celular e Molecular) - Universidade Federal da Paraí­ba, João Pessoa, 2011.
dc.identifier.uri.fl_str_mv https://repositorio.ufpb.br/jspui/handle/tede/3640
identifier_str_mv VASCONCELOS, Danielle Ingrid Bezerra de. Análise do efeito imunomodulador da ouabaína na inflamação e nocicepção. 2011. 144 f. Dissertação (Mestrado em Biologia Celular e Molecular) - Universidade Federal da Paraí­ba, João Pessoa, 2011.
url https://repositorio.ufpb.br/jspui/handle/tede/3640
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Universidade Federal da Paraí­ba
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Biologia Celular e Molecular
dc.publisher.initials.fl_str_mv UFPB
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Biologia Celular e Molecular
publisher.none.fl_str_mv Universidade Federal da Paraí­ba
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFPB
instname:Universidade Federal da Paraíba (UFPB)
instacron:UFPB
instname_str Universidade Federal da Paraíba (UFPB)
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institution UFPB
reponame_str Repositório Institucional da UFPB
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