Efeito do laser de baixa potência na proliferação de fibroblastos pulpares humanos e na atividade das metaloproteinases 2 e 9 in vitro

• Ano de defesa: 2006
• Autor(a) principal: Carvalho, Rodrigo Varella de
• Orientador(a): Etges, Adriana
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Pelotas
• Programa de Pós-Graduação: Programa de Pós-Graduação em Odontologia
• Departamento: Odontologia
• País: BR
• Palavras-chave em Português: Dentística; Terapia a laser de baixa intensidade; Metaloproteinases da matriz; Fibroblastos; Técnicas de cultura de células
• Palavras-chave em Inglês: Low power laser; Metalloproteinases; Fibroblasts
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: https://guaiaca.ufpel.edu.br/handle/123456789/2219

The aim of the present study was to evaluate the gelatinase activity (MMP-2 and 9), and proliferation of human pulp fibroblasts in vitro, after irradiation with a low level diode laser (780nm). The activity was evaluated with zymography and the proliferation rate was obtained through growth curves made in SigmaPlot (8.0), after the counting in Neubauer chamber.in light microscope. Cells used to zimography were cultived in DMEM, 10% fetal bovine serum and incubated at 37ºC in atmosphere of 5% CO2 and 95% air, in 100% humidity. Cells used to proliferation rate were cultivated in nutritional deficit (5% SFB). Groups to zymography and cell proliferation were determined: zymography - Z1 = not irradiated, Z2 = 3J/cm2 e Z3 = 6J/cm2, cell proliferation: L1 = not irradiated, L2 = 3J/cm2 e L3 = 6J/cm2. The counting was made after 2, 4 and 6 days. Assays were performed in triplicate. The zymography showed that the MMP-2 activity was major with MMP-9, and, Z2 e Z3 produced a higher activity than MMP-2, when compared to Z1. Results of the zymography and proliferation test were compared by either ANOVA complemented by Tukey´s test. The level of significance was 5% (p<0,05). Statistical analysis demonstrate difference between the groups in different days. The L3 obtained higher proliferation in the finish of the six days. L2 demonstrated higher growth than L1. Irradiated fibroblasts with the low power laser expressed a higher amount of MMP-2 activity and 6J/cm2 produced a higher proliferation rate in the initial days