Development of bioprocesses for the production of a biological indicator for sterilization processes from Bacillus atrophaeus spores

Detalhes bibliográficos
Ano de defesa: 2012
Autor(a) principal: Sella, Sandra Regina Barroso Ruiz
Orientador(a): Soccol, Carlos Ricardo, 1953-
Banca de defesa: Não Informado pela instituição
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Não Informado pela instituição
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Bacillus (Bacteria)
Esterilização
Indicadores (Biologia)
Link de acesso: https://hdl.handle.net/1884/29811
Resumo: Orientador: Prof. Dr. Carlos Ricardo Soccol
id UFPR_bef1cc5aa2a889789c62a0804c35cbe5
oai_identifier_str oai:acervodigital.ufpr.br:1884/29811
network_acronym_str UFPR
network_name_str Repositório Institucional da UFPR
repository_id_str
spelling Vandenberghe, Luciana Porto de Souza, 1968-Minozzo, Joao CarlosUniversidade Federal do Paraná. Setor de Tecnologia. Programa de Pós-Graduação em Engenharia de Bioprocessos e BiotecnologiaSoccol, Carlos Ricardo, 1953-Sella, Sandra Regina Barroso Ruiz2024-04-11T18:50:43Z2024-04-11T18:50:43Z2012https://hdl.handle.net/1884/29811Orientador: Prof. Dr. Carlos Ricardo SoccolCoorientadores: Profa. Dra. Luciana P. S. Vandenberghe e Prof. Dr. João Carlos MinozzoTese (doutorado) - Universidade Federal do Paraná, Setor de Tecnologia, Programa de Pós-Graduação em Engenharia de Bioprocessos e Biotecnologia. Defesa: Curitiba, 22/11/2012Bibliografia: fls. 228-232Área de concentração: Saúde humana e animalAbstract: The genus Bacillus includes a great diversity of industrially important strains, including Bacillus atrophaeus (formerly Bacillus subtilis var. niger). This spore-forming bacterium has been established as industrial bacteria in the production of biological sterilization indicators, in studies of biodefense and astrobiology methods, and as potential adjuvants or vehicles for vaccines, among other applications. Two novels, cost-effective B. atrophaeus Sterilization Bioindicator Systems (BIS) with high quality and performance were developed from soybean molasses and glycerol and compared with commercial BIS. The BISs were composed of a recovery medium and dry-fermented. The production of biological indicators involving bacterial sporulation and multi-step downstream processes has been described. The first goal of the present work was to use fermented material as the final product in the BIS, thereby reducing processing steps and costs. The performance of three different inexpensive supports (vermiculite, sand and sugarcane bagasse) was assessed by determining B. atrophaeus sporulation during solid-state fermentation (SSF). Sand proved to be the best inert support, which enabled the direct use of the fermented product due to its easy homogenization, filling properties, and compatibility with the recovery medium. The BISs were developed and optimized using a sequential experimental design strategy. For soybean-based BIS, the optimum recovery medium contained soluble starch (1.0 g/L), soybean molasses (30.0 g/L), tryptone (40.0 g/L), and bromothymol blue (0.02 g/L) at pH 8.5. The SSF conditions of the bioreactor and environmental humidity had no significant impact on spore yield and dry-heat resistance. The only substrate mineral that showed a positive effect was Mn2+, allowing Mg2+, K+, and Ca2+ to be eliminated from the formulation. Validation of optimized medium indicated a D160°C = 6.8 ± 1.0 min (3.6 min more than the minimum) and a spore yield = 2.3 ± 0.5 × 109 CFU/g dry sand (10,000 × initial values). Cost reduction was of 23.9% and process cycle time was also reduced from 29 to 15 days. The study of the growth characteristics and the metabolic and enzymatic profiles confirmed that sporulation through SSF of B. atrophaeus occurs by biofilm formation and that this model of fermentation promotes important phenotypic changes in the spores. This study proposes a new concept regarding bacterial biofilm formation by SSF. For glycerol-based BIS, the proposed recovery medium enables the germination and outgrowth of heat-damaged spores, promoting a D160ºC value of 6.6 ? 0.1 min. B. atrophaeus spore production by SSF reached 2.3 ± 1.2 x 108 CFU/g dry matter. Sporulation kinetics results showed that only 5 days were sufficient for this process. Cost breakdowns were from 41.8% (quality control) up to 72.8% (feedstock). A performance evaluation of the proposed BIS against dry-heat and ethylene oxide sterilization showed compliance with regulatory requirements. Microwave disinfection tests demonstrated that the developed BISs were more resistant than the control BIS. Additional studies are necessary to determine if this is a positive factor for microwave medical waste treatment monitoring or if it may cause a false process failure indication. These processes may be utilized for spore production aimed at other applications or for the cost-effective production of spores from other Bacillus species.232f. : il. [algumas color.], grafs., tabs.application/pdfDisponível em formato digitalBacillus (Bacteria)EsterilizaçãoIndicadores (Biologia)Development of bioprocesses for the production of a biological indicator for sterilization processes from Bacillus atrophaeus sporesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisporreponame:Repositório Institucional da UFPRinstname:Universidade Federal do Paraná (UFPR)instacron:UFPRinfo:eu-repo/semantics/openAccessORIGINALR - T - SANDRA REGINA BARROSO RUIZ SELLA.pdfapplication/pdf5559484https://acervodigital.ufpr.br/bitstream/1884/29811/1/R%20-%20T%20-%20SANDRA%20REGINA%20BARROSO%20RUIZ%20SELLA.pdf3040dfd140f57fe1a5d6e7959129f88aMD51open accessTEXTR - T - SANDRA REGINA BARROSO RUIZ SELLA.pdf.txtExtracted Texttext/plain431639https://acervodigital.ufpr.br/bitstream/1884/29811/2/R%20-%20T%20-%20SANDRA%20REGINA%20BARROSO%20RUIZ%20SELLA.pdf.txt08e6f2ca94985cba87cd400d9dc5204cMD52open accessTHUMBNAILR - T - SANDRA REGINA BARROSO RUIZ SELLA.pdf.jpgGenerated Thumbnailimage/jpeg1142https://acervodigital.ufpr.br/bitstream/1884/29811/3/R%20-%20T%20-%20SANDRA%20REGINA%20BARROSO%20RUIZ%20SELLA.pdf.jpgf999a2e225374aff22915e488195ca64MD53open access1884/298112024-04-11 15:50:43.298open accessoai:acervodigital.ufpr.br:1884/29811Repositório InstitucionalPUBhttp://acervodigital.ufpr.br/oai/requestinformacaodigital@ufpr.bropendoar:3082024-04-11T18:50:43Repositório Institucional da UFPR - Universidade Federal do Paraná (UFPR)false
dc.title.pt_BR.fl_str_mv Development of bioprocesses for the production of a biological indicator for sterilization processes from Bacillus atrophaeus spores
title Development of bioprocesses for the production of a biological indicator for sterilization processes from Bacillus atrophaeus spores
spellingShingle Development of bioprocesses for the production of a biological indicator for sterilization processes from Bacillus atrophaeus spores
Sella, Sandra Regina Barroso Ruiz
Bacillus (Bacteria)
Esterilização
Indicadores (Biologia)
title_short Development of bioprocesses for the production of a biological indicator for sterilization processes from Bacillus atrophaeus spores
title_full Development of bioprocesses for the production of a biological indicator for sterilization processes from Bacillus atrophaeus spores
title_fullStr Development of bioprocesses for the production of a biological indicator for sterilization processes from Bacillus atrophaeus spores
title_full_unstemmed Development of bioprocesses for the production of a biological indicator for sterilization processes from Bacillus atrophaeus spores
title_sort Development of bioprocesses for the production of a biological indicator for sterilization processes from Bacillus atrophaeus spores
author Sella, Sandra Regina Barroso Ruiz
author_facet Sella, Sandra Regina Barroso Ruiz
author_role author
dc.contributor.other.pt_BR.fl_str_mv Vandenberghe, Luciana Porto de Souza, 1968-
Minozzo, Joao Carlos
Universidade Federal do Paraná. Setor de Tecnologia. Programa de Pós-Graduação em Engenharia de Bioprocessos e Biotecnologia
dc.contributor.advisor1.fl_str_mv Soccol, Carlos Ricardo, 1953-
dc.contributor.author.fl_str_mv Sella, Sandra Regina Barroso Ruiz
contributor_str_mv Soccol, Carlos Ricardo, 1953-
dc.subject.por.fl_str_mv Bacillus (Bacteria)
Esterilização
Indicadores (Biologia)
topic Bacillus (Bacteria)
Esterilização
Indicadores (Biologia)
description Orientador: Prof. Dr. Carlos Ricardo Soccol
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2024-04-11T18:50:43Z
dc.date.available.fl_str_mv 2024-04-11T18:50:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1884/29811
url https://hdl.handle.net/1884/29811
dc.language.iso.fl_str_mv por
language por
dc.relation.pt_BR.fl_str_mv Disponível em formato digital
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 232f. : il. [algumas color.], grafs., tabs.
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFPR
instname:Universidade Federal do Paraná (UFPR)
instacron:UFPR
instname_str Universidade Federal do Paraná (UFPR)
instacron_str UFPR
institution UFPR
reponame_str Repositório Institucional da UFPR
collection Repositório Institucional da UFPR
bitstream.url.fl_str_mv https://acervodigital.ufpr.br/bitstream/1884/29811/1/R%20-%20T%20-%20SANDRA%20REGINA%20BARROSO%20RUIZ%20SELLA.pdf
https://acervodigital.ufpr.br/bitstream/1884/29811/2/R%20-%20T%20-%20SANDRA%20REGINA%20BARROSO%20RUIZ%20SELLA.pdf.txt
https://acervodigital.ufpr.br/bitstream/1884/29811/3/R%20-%20T%20-%20SANDRA%20REGINA%20BARROSO%20RUIZ%20SELLA.pdf.jpg
bitstream.checksum.fl_str_mv 3040dfd140f57fe1a5d6e7959129f88a
08e6f2ca94985cba87cd400d9dc5204c
f999a2e225374aff22915e488195ca64
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFPR - Universidade Federal do Paraná (UFPR)
repository.mail.fl_str_mv informacaodigital@ufpr.br
_version_ 1847526034297061376

Registros relacionados