Influência do cólon na regeneração hepática de ratos submetidos a hepatectomia e colectomia

• Ano de defesa: 2017
• Autor(a) principal: Moreira, Marilia Daniela Ferreira de Carvalho
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Brasil; UFRN; PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS DA SAÚDE
• Palavras-chave em Português: Regeneração hepática; Colectomia; Hepatectomia; Cólon; CNPQ::CIENCIAS DA SAUDE
• Link de acesso: https://repositorio.ufrn.br/jspui/handle/123456789/24785

Studies on the influence of the colon on liver regeneration are scarce. Some have shown that after colectomy there is an increase in regeneration and its reduction in others. Faced with the controversy, this work aimed to investigate whether colectomy simultaneously performed with 70% hepatectomy influences liver regeneration in rats. Eighteen Wistar rats were distributed in 3 groups, 6 animals each. In group I (sham) only laparotomy was performed with mild manipulation of boweld loops; in group II 70% hepatectomy and simultaneous colectomy; in group III only 70% hepatectomy. On the 6th postoperative day, blood was collected by cardiac puncture under anesthesia, and remaining liver was removed. Serum measures of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (FA), albumin, hepatocyte growth factor (HGF) and transforming growth factor-α (TGF-α) were performed. Liver regeneration was calculated by (liver weight per 100g of the body weight at sacrifice/preoperative projected liver weight per 100g of the body weight) × 100. The Analyses of variance (ANOVA) and Tukey tests were used, with significance p<0.05. There was significant lower levels of ALT and AST in group II rats when compared to group III (p<0.001). Albuminemia showed significantly higher levels in group II rats than in III (p<0.01). Serum levels of HGF (408±18.2pg/mL) and TGF-α (3.8±0.3ng/mL) were significantly higher in group II rats than HGF (360±58.6pg/mL), TGF-α (2.3±0.4ng/mL) in group III, respectively. The percentage of liver regeneration was significantly higher in group II than in group III (p = 0.003). In conclusion, this study demonstrated that colectomy, performed simultaneously with 70% hepatectomy, positively influenced liver regeneration in rats. Further research is needed to reveal the molecular mechanisms of this effect and to characterize the effects of the colon on liver physiology.