Desenvolvimento e avaliação físico-química e mecânica de membranas de quitosana contendo sistemas multicomponentes de triancinolona acetonida

• Ano de defesa: 2020
• Autor(a) principal: Nascimento, Ednaldo Gomes do
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Rio Grande do Norte; Brasil; UFRN; PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS FARMACÊUTICAS
• Palavras-chave em Português: Triancinolona acetonida; Membranas de quitosana; Ciclodextrinas; Estomatite aftosa; Liberação controlada de fármaco
• Link de acesso: https://repositorio.ufrn.br/handle/123456789/30419

The physical, mental and social well-being of individuals has been the pillar of the study of science to improve the quality of life for humanity. One of the key indicators for this well-being is oral health. However, almost half of the world's population is affected by diseases in the oral cavity, causing lesions in its mucosa, such as recurrent aphthous stomatitis. Triamcinolone acetonide is a corticosteroid that is used to treat these oral ulcerations. Nevertheless, it is usually used as ointments and creams, which requires a repeated applications and limited residence time of drug in the oral cavity. Hydrophilic polymeric films have great potential to solve these adversities. Among the polymers, chitosan has been used due to their important therapeutic properties, such as antimicrobial, antiulcerative, and healing actions. However, triamcinolone acetonide is a drug with low solubility in water, which limits its incorporation into hydrophilic films. The aim of this study was developing chitosan membranes containing triamcinolone acetonide. For this, multicomponent systems with drug, cyclodextrins, and triethanolamine were tested to make possible the preparation of membranes able to supply sustained release of drug. The chitosan membranes were prepared by the solvent evaporation method, in which the entrapment of drug was performed through the prior dispersion of this drug in solutions containing hydroxypropyl beta-cyclodextrin or beta-cyclodextrin (binary systems) or even containing triethanolamine (ternary system). The membranes were characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM-FEG), infrared spectroscopy with Fourier transform (FTIR), swelling assays, measurements of surface pH, X-ray diffraction (XRD), tensile assay, and thermal analysis (DSC and TG/DTG). The quantitative analysis of the drug released from the membranes was performed by highefficiency liquid chromatography (UHPLC). All proposed systems presented physicalchemical characteristics with some singularities. Nevertheless, the ternary system containing triethanolamine stood out with the best properties. The in vitro release performance assay of triamcinolone acetonide was performed in Franz-type diffusion cells, and the mathematical treatment of the data revealed better fit by Bhaskar and parabolic diffusion models, which corroborates a two-phase drug release controlled by diffusion. The presence of triethanolamine in the chitosan membrane (ternary system) was able to modify and modulate the release of triamcinolone acetonide. This system proved to be an efficient and promising device for the release of low solubility drugs in topical administration such as triamcinolone acetonide and its potential use in the oral cavity.