Transcriptoma do abomaso de ovinos e possíveis mecanismos de resposta a Haemonchus contortus

• Ano de defesa: 2017
• Autor(a) principal: Silva, Iasmim Santos Mangabeira e
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Brasil; UFRN; PROGRAMA DE PÓS-GRADUAÇÃO EM PRODUÇÃO ANIMAL
• Palavras-chave em Português: Alimentação de animais; Endoparasitas; Ovinocultura; Resposta imune; Resistência; Sequenciamento do RNA mensageiro; CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::PRODUCAO ANIMAL
• Link de acesso: https://repositorio.ufrn.br/jspui/handle/123456789/24075

The aim of this study was to understand molecular mechanisms underlying host resistance. We compared the abomasal mucosal transcriptome of 17 crossbred ½ Santa Inês and ½ Dorper lambs, previously classified as infected (resistant and susceptible) and uninfected distributed in two feeding system (ad libitum and restrict feed) in response to Haemonchus sp. infection using RNA-Seq technology. The libraries preparation, genome sequencing and sequence analyses were performed at the Laboratory of Animal Biotechnology - ESALQ, Piracicaba, Brazil. The average of reads per sample before and after filtering was 12.522.573 and 9.626.457, respectively, and the average of mapping rate of filtered reads against to Ovis aries Oar_v4.0 reference genome assembly was 79.66%. 421 and 1123 differentially expressed (DE) genes were identified when compared to infected and uninfected animals, within the restricted and ad libitum feed groups, respectively. When evaluated the infected animals inserted in the ad libitum feed versus restricted feeding, 13 genes were DE. When evaluated the resistant and susceptible animals with fixed feeding effect, only 36 genes were DE. Finally, by comparing infected versus control animals with feed as a fixed effect, 881 DE genes were identified. Functional enrichment analysis showed that some Gene Ontology terms were significantly enriched (adjusted p-value <0.05). Our findings suggest that in addition to genes that participate directly in the immune system, genes that participate in other biological pathways such as arachidonic acid metabolism, signaling pathway and complement system, for example, are essential in the host's response to Haemonchus contortus, generating a greater sheep resistance. In addition, feeding did not have a significant effect on the gene expression profile, showing that the difference between gene expressions was due to H. contortus infection.