Avalia??o de diferentes press?es negativas na aspira??o folicular transvaginal guiada por ultrassom sobre a recupera??o oocit?ria em ?guas

• Ano de defesa: 2012
• Autor(a) principal: S?, Marcus Andr? Ferreira
• Orientador(a): Jacob, Julio Cesar Ferraz
• Banca de defesa: Jacob, J?lio C?sar Ferraz, O?a, Cely Marini Melo e, Dell'Aqua Junior, Jos? Ant?nio
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal Rural do Rio de Janeiro
• Programa de Pós-Graduação: Programa de P?s-Gradua??o em Medicina Veterin?ria (Patologia e Ci?ncias Cl?nicas)
• Departamento: Instituto de Veterin?ria
• País: Brasil
• Palavras-chave em Português: OPU; o?cito; press?o negativa
• Palavras-chave em Inglês: OPU; oocyte; negative pressure
• Área do conhecimento CNPq: Medicina Veterin?ria
• Link de acesso: https://tede.ufrrj.br/jspui/handle/jspui/1514

The present experiment aimed to verify if different vacuum pump negative pressures (150, 280 e 400 mmHg) can influence the oocyte recovery rate per preovulatory follicle aspirated. Hence, 21 estrous cycles from regularly cycling mares were subject to OPU. The estrous cycles were sorted in three groups (G150=150 mmHg; G280=280 mmHg; G400=400 mmHg) in the following sequence: G150= 150mmHg (n=6); G280= 280mmHg (n=7); G400= 400mmHg (n=6). During the estrus, the ovarian activity of the mares was daily monitored using the transrectal ultrasound technique until the largest follicle reached at least a diameter of 35mm and endometrial edema secore 2.5 was identified on ultrasonographic evaluation, when 1000UI of hCG administered intravenuous. Approximately 24 hours after the hCG administered, the mares were monitored by rectal palpation and ultrasonography every six hours to follicle evaluation. In the case of imminent indication of ovulation or formation of hemorrhagic follicle, the follicle would be immediately aspirate. The aspirations occurred in 32,45?1,92h after the hCG administration. The transvaginal aspirations were performed with ultrasound apparatus equipped with a convex transducer of 5,0mHz with polyethylene guide containing a double lumen needle of 12G. The follicular fluid collected from each follicle was frozen and the aspirated content was transferred to a Petri Dishes and thoroughly examined on the stereomicroscope to identify the oocytes presence. In order to statistically evaluate the influence of different pressures on the oocyte recovery, were used Chi-Squared test (a 5% significance) and Fisher Exact Test, when recommended. The recovery rate was 31,57% (6/19), being 16,66 % (1/6) in G150, 42,85 % (3/7) in G280 and 33,33 % (2/6) in G400. There was no difference among groups (p>0,05). From the results of the current study, it is possible to conclude that the negative pressure of the vacuum pump is not a determining factor to increase the oocyte recovery and other aspects would possibly have more significant influence .