?leos essenciais e extratos vegetais de plantas cultivadas no Brasil: impacto no crescimento de Aspergillus ochraceus e Aspergillus carbonarius
Ano de defesa: | 2010 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal Rural do Rio de Janeiro
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Ci?ncias Veterin?rias
|
Departamento: |
Instituto de Veterin?ria
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://tede.ufrrj.br/jspui/handle/tede/796 |
Resumo: | Essential oils and plant extracts from aromatic plants are recognized for its antimicrobial properties and effectiveness as food antioxidants. The mycotoxins are toxic metabolites produced by filamentous fungi in feed, being particularly harmful to animals and humans. The mycotoxin occurrence happens after an oxidation process, consequently, the oxidation prevention is a way to avoid mycotoxins production, like ochratoxin A, which is a nephrotoxic toxin, mainly produced by Aspergillus ochraceus, Aspergillus niger and Aspergillus carbonarius in tropical areas. There is evidence that syntetic antioxidants can be prejudicial to animals and humans health. Thus, this study aimed to evaluate the effectiveness of Brazilian plant extracts (essential oils hydrodistillation and hexanic extraction; and vegetal extracts: aqueous and ethanolic) to inhibit the growth of A. ochraceus NRRL 3174 and A. carbonarius RC 2054 (UNRC). A total of 40 plant extracts from ten vegetable species: basil (Ocimum basilicum), cinnamon (Cinnamomum zeylanicum), clove (Eugenia caryophyllata), cumin (Cuminum cyminum), marjoram (Origanum majorana), nutmeg (Myristica fragrans), oregano (Origanum vulgare), rosemary (Rosmarinus officinalis), spearmint (Menta piperita) and sweet fennel (Pimpinella anisum) were screened by diffusion agar test for the best results on mycelial growth inhibition. Oregano essential oils (obtained by hydrodistillation and hexanic extraction), the rosemary essential oil and the clove ethanolic extract were chosen to obtain the growth rate and the lag phase at concentrations of 0, 50, 100, 150, 300 and 600 mg/kg on yeast extract-sucrose agar (YES). Strains were centrally inoculated and the radial growth (mm/day) was daily measured. The growth rate decreased as the essential oil concentration increased in all treatments and fungal strains assayed. The oregano essential oil showed the best results among the other essential oils. Comparing all four treatments, the best result was for clove ethanolic extract and the worst one was for oregano essential oil produced by hexanic extraction. The concentration of 600 mg/kg exerted the best inhibitory effect. These results are interesting related to the prevention of fungi contamination in many foods and they could be used instead of synthetic antifungal products. Future studies should be conducted to determine the ability of these oils and extracts to reduce the ochratoxin A production. |
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Rosa, Carlos Alberto da RochaCPF:36263753749http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787219Z7&dataRevisao=nullCPF:10378921789http://lattes.cnpq.br/5161284526947642Oliveira, ?guida Aparecida de2016-04-28T20:15:37Z2011-04-112010-02-09OLIVEIRA, ?guida Aparecida de. ?leos essenciais e extratos vegetais de plantas cultivadas no Brasil: impacto no crescimento de Aspergillus ochraceus e Aspergillus carbonarius. 2010. 61 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2010.https://tede.ufrrj.br/jspui/handle/tede/796Essential oils and plant extracts from aromatic plants are recognized for its antimicrobial properties and effectiveness as food antioxidants. The mycotoxins are toxic metabolites produced by filamentous fungi in feed, being particularly harmful to animals and humans. The mycotoxin occurrence happens after an oxidation process, consequently, the oxidation prevention is a way to avoid mycotoxins production, like ochratoxin A, which is a nephrotoxic toxin, mainly produced by Aspergillus ochraceus, Aspergillus niger and Aspergillus carbonarius in tropical areas. There is evidence that syntetic antioxidants can be prejudicial to animals and humans health. Thus, this study aimed to evaluate the effectiveness of Brazilian plant extracts (essential oils hydrodistillation and hexanic extraction; and vegetal extracts: aqueous and ethanolic) to inhibit the growth of A. ochraceus NRRL 3174 and A. carbonarius RC 2054 (UNRC). A total of 40 plant extracts from ten vegetable species: basil (Ocimum basilicum), cinnamon (Cinnamomum zeylanicum), clove (Eugenia caryophyllata), cumin (Cuminum cyminum), marjoram (Origanum majorana), nutmeg (Myristica fragrans), oregano (Origanum vulgare), rosemary (Rosmarinus officinalis), spearmint (Menta piperita) and sweet fennel (Pimpinella anisum) were screened by diffusion agar test for the best results on mycelial growth inhibition. Oregano essential oils (obtained by hydrodistillation and hexanic extraction), the rosemary essential oil and the clove ethanolic extract were chosen to obtain the growth rate and the lag phase at concentrations of 0, 50, 100, 150, 300 and 600 mg/kg on yeast extract-sucrose agar (YES). Strains were centrally inoculated and the radial growth (mm/day) was daily measured. The growth rate decreased as the essential oil concentration increased in all treatments and fungal strains assayed. The oregano essential oil showed the best results among the other essential oils. Comparing all four treatments, the best result was for clove ethanolic extract and the worst one was for oregano essential oil produced by hexanic extraction. The concentration of 600 mg/kg exerted the best inhibitory effect. These results are interesting related to the prevention of fungi contamination in many foods and they could be used instead of synthetic antifungal products. Future studies should be conducted to determine the ability of these oils and extracts to reduce the ochratoxin A production.?leos essenciais e extratos vegetais de plantas arom?ticas s?o reconhecidos por suas propriedades antimicrobianas e efici?ncia como antioxidante de alimentos. As micotoxinas s?o metab?litos t?xicos produzidos por fungos filamentosos em alimentos, sendo particularmente nocivas a animais e humanos. A ocorr?ncia de micotoxinas ocorre depois de um processo de oxida??o, consequentemente, a preven??o da oxida??o ? um meio de evitar a produ??o de micotoxinas, como a ocratoxina A, que ? uma toxina nefrot?xica, principalmente produzida por Aspergillus ochraceus, Aspergillus niger e Aspergillus carbonarius em ?reas tropicais. H? evid?ncias de que antioxidantes sint?ticos possam ser prejudiciais ? sa?de animal e humana. Assim, este estudo objetivou avaliar o efeito inibit?rio de plantas arom?ticas brasileiras (?leos essenciais obtidos por hidrodestila??o e extra??o hex?nica; e extratos vegetais: etan?lico e aquoso) sobre o crescimento de A. ochraceus NRRL 3174 e A. carbonarius RC 2054 (UNRC). Um total de 40 extratos de plantas oriundos de dez esp?cies vegetais: manjeric?o (Ocimum basilicum), canela (Cinnamomum zeylanicum), cravo (Eugenia caryophyllata), cominho (Cuminum cyminum), manjerona (Origanum majorana), noz moscada (Myristica fragrans), or?gano (Origanum vulgare), alecrim (Rosmarinus officinalis), hortel? (Menta piperita) e erva-doce (Pimpinella anisum) foram submetidos a uma sele??o pelo teste de difus?o em agar para eleger os que proporcionassem os maiores halos de inibi??o f?ngica. Os ?leos essenciais de or?gano (obtidos por hidrodestila??o e extra??o hex?nica), de alecrim (obtido por hidrodestila??o) e o extrato etan?lico de cravo foram os mais efetivos, e foram ent?o escolhidos para a obten??o dos par?metros de velocidade de crescimento e fase lag nas concentra??es de 0, 50, 100, 150, 300 e 600 mg/kg em placas de Petri contendo meio agar extrato de levedura e sacarose (yeast extract sucrose agar - YES); as cepas foram inoculadas no ponto central, e o crescimento radial da col?nia (mm/dia) foi mensurado diariamente. A velocidade de crescimento diminu?a ? medida que a concentra??o do ?leo essencial aumentava em todos os tratamentos e cepas testadas. O ?leo essencial de or?gano mostrou os melhores resultados dentre os ?leos essenciais. Comparando os quatro tratamentos, os melhores resultados foram obtidos com o extrato etan?lico de cravo, e os piores resultados com o ?leo essencial de or?gano obtido por extra??o hex?nica. A concentra??o de 600 mg/kg foi a de maior poder inibit?rio. Esses resultados s?o interessantes na conex?o com a preven??o do crescimento f?ngico em muitos alimentos, e poder?o ser usados em substitui??o dos produtos antif?ngicos sint?ticos. Mais estudos devem ser conduzidos para determinar a habilidade desses ?leos e extratos na redu??o da produ??o de ocratoxina A.Made available in DSpace on 2016-04-28T20:15:37Z (GMT). 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dc.title.por.fl_str_mv |
?leos essenciais e extratos vegetais de plantas cultivadas no Brasil: impacto no crescimento de Aspergillus ochraceus e Aspergillus carbonarius |
dc.title.alternative.eng.fl_str_mv |
Brazilian essential oils and plant extracts: impact on growth of Aspergillus ochraceus and Aspergillus carbonarius |
title |
?leos essenciais e extratos vegetais de plantas cultivadas no Brasil: impacto no crescimento de Aspergillus ochraceus e Aspergillus carbonarius |
spellingShingle |
?leos essenciais e extratos vegetais de plantas cultivadas no Brasil: impacto no crescimento de Aspergillus ochraceus e Aspergillus carbonarius Oliveira, ?guida Aparecida de fungos micotoxinas antioxidantes naturais fungi mycotoxins natural antioxidants Medicina Veterin?ria |
title_short |
?leos essenciais e extratos vegetais de plantas cultivadas no Brasil: impacto no crescimento de Aspergillus ochraceus e Aspergillus carbonarius |
title_full |
?leos essenciais e extratos vegetais de plantas cultivadas no Brasil: impacto no crescimento de Aspergillus ochraceus e Aspergillus carbonarius |
title_fullStr |
?leos essenciais e extratos vegetais de plantas cultivadas no Brasil: impacto no crescimento de Aspergillus ochraceus e Aspergillus carbonarius |
title_full_unstemmed |
?leos essenciais e extratos vegetais de plantas cultivadas no Brasil: impacto no crescimento de Aspergillus ochraceus e Aspergillus carbonarius |
title_sort |
?leos essenciais e extratos vegetais de plantas cultivadas no Brasil: impacto no crescimento de Aspergillus ochraceus e Aspergillus carbonarius |
author |
Oliveira, ?guida Aparecida de |
author_facet |
Oliveira, ?guida Aparecida de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Rosa, Carlos Alberto da Rocha |
dc.contributor.advisor1ID.fl_str_mv |
CPF:36263753749 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787219Z7&dataRevisao=null |
dc.contributor.authorID.fl_str_mv |
CPF:10378921789 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5161284526947642 |
dc.contributor.author.fl_str_mv |
Oliveira, ?guida Aparecida de |
contributor_str_mv |
Rosa, Carlos Alberto da Rocha |
dc.subject.por.fl_str_mv |
fungos micotoxinas antioxidantes naturais |
topic |
fungos micotoxinas antioxidantes naturais fungi mycotoxins natural antioxidants Medicina Veterin?ria |
dc.subject.eng.fl_str_mv |
fungi mycotoxins natural antioxidants |
dc.subject.cnpq.fl_str_mv |
Medicina Veterin?ria |
description |
Essential oils and plant extracts from aromatic plants are recognized for its antimicrobial properties and effectiveness as food antioxidants. The mycotoxins are toxic metabolites produced by filamentous fungi in feed, being particularly harmful to animals and humans. The mycotoxin occurrence happens after an oxidation process, consequently, the oxidation prevention is a way to avoid mycotoxins production, like ochratoxin A, which is a nephrotoxic toxin, mainly produced by Aspergillus ochraceus, Aspergillus niger and Aspergillus carbonarius in tropical areas. There is evidence that syntetic antioxidants can be prejudicial to animals and humans health. Thus, this study aimed to evaluate the effectiveness of Brazilian plant extracts (essential oils hydrodistillation and hexanic extraction; and vegetal extracts: aqueous and ethanolic) to inhibit the growth of A. ochraceus NRRL 3174 and A. carbonarius RC 2054 (UNRC). A total of 40 plant extracts from ten vegetable species: basil (Ocimum basilicum), cinnamon (Cinnamomum zeylanicum), clove (Eugenia caryophyllata), cumin (Cuminum cyminum), marjoram (Origanum majorana), nutmeg (Myristica fragrans), oregano (Origanum vulgare), rosemary (Rosmarinus officinalis), spearmint (Menta piperita) and sweet fennel (Pimpinella anisum) were screened by diffusion agar test for the best results on mycelial growth inhibition. Oregano essential oils (obtained by hydrodistillation and hexanic extraction), the rosemary essential oil and the clove ethanolic extract were chosen to obtain the growth rate and the lag phase at concentrations of 0, 50, 100, 150, 300 and 600 mg/kg on yeast extract-sucrose agar (YES). Strains were centrally inoculated and the radial growth (mm/day) was daily measured. The growth rate decreased as the essential oil concentration increased in all treatments and fungal strains assayed. The oregano essential oil showed the best results among the other essential oils. Comparing all four treatments, the best result was for clove ethanolic extract and the worst one was for oregano essential oil produced by hexanic extraction. The concentration of 600 mg/kg exerted the best inhibitory effect. These results are interesting related to the prevention of fungi contamination in many foods and they could be used instead of synthetic antifungal products. Future studies should be conducted to determine the ability of these oils and extracts to reduce the ochratoxin A production. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-02-09 |
dc.date.available.fl_str_mv |
2011-04-11 |
dc.date.accessioned.fl_str_mv |
2016-04-28T20:15:37Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, ?guida Aparecida de. ?leos essenciais e extratos vegetais de plantas cultivadas no Brasil: impacto no crescimento de Aspergillus ochraceus e Aspergillus carbonarius. 2010. 61 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2010. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/tede/796 |
identifier_str_mv |
OLIVEIRA, ?guida Aparecida de. ?leos essenciais e extratos vegetais de plantas cultivadas no Brasil: impacto no crescimento de Aspergillus ochraceus e Aspergillus carbonarius. 2010. 61 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2010. |
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https://tede.ufrrj.br/jspui/handle/tede/796 |
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por |
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Universidade Federal Rural do Rio de Janeiro |
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Universidade Federal Rural do Rio de Janeiro |
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