M?todos de diagn?stico em Fasciola hepatica (Trematoda: Fasciolidae)

• Ano de defesa: 2014
• Autor(a) principal: Carneiro, Milena Batista
• Orientador(a): Scott, Fabio Barbour
• Banca de defesa: Scott, Fabio Barbour, Martins, Isabella Vilhena Freire, Lopes, Carlos Wilson Gomes, Nunes, Louisiane de Carvalho, Leite, Ronaldo Cerqueira
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal Rural do Rio de Janeiro
• Programa de Pós-Graduação: Programa de P?s-Gradua??o em Ci?ncias Veterin?rias
• Departamento: Instituto de Veterin?ria
• País: Brasil
• Palavras-chave em Português: fasciolose; Lymnaea columella; epidemiologia
• Palavras-chave em Inglês: fasciolosis; epidemiology
• Área do conhecimento CNPq: Medicina Veterin?ria
• Link de acesso: https://tede.ufrrj.br/jspui/handle/jspui/3095

This study investigated methods of diagnosis and control of Fasciola hepatica in ruminants, in addition provid information about the population dynamics of Lymnaea columella. In the first stage of labor, between November 2010 and June 2013, the mollusc species L. columella were collected at five diferent locations and comper with Meteorological data such as precipitation and temperature. There was no significant diference between the mean number of mollusks at the seasons. The second stage of the study involved the comparison of the sedimentation technique described by Foreyt (2005) with four sieves technique proposed by Gir?o; Ueno (1985) with artificially infected cattle feces with F. hepatica eggs for quantitative diagnosis of this parasite. After statistical analysis, the sedimentation technique showed better results with higher percentages of recovery in addition to having lower cost and be easier to perform than the sieving technique. In the third and final stage of this study, adult specimens of F. hepatica were collected from sheep previously treated with albendazole and one sheep control. The evaluation times were 48, 72 and 96 hours post- treatment. Flukes were processed for histopathologic and assessed for changes in male reproductive sistem. It was observed that during the evaluation period were significant changes in spermatogenic cells which include rarefaction cell, apoptosis in cells with typical pyknosis and karyorrhexis and thereby decrease sperm production, as well as intra-and extracellular vacuolation. These results demonstrate that albendazole altered sperm production which interferes with the production of fluke eggs, demonstrating the effectiveness of the drug in this experiment. Studies of seasonality intermediate host of F. hepatica, which contribute to the choice of the best diagnostic technique of this parasite and demonstrating efficacy and / or drug resistance are essential for the establishment, in each region, an efficient strategic program of fluke control.